Epitope motif of an anti-nitrotyrosine antibody specific for tyrosine-nitrated peptides revealed by a combination of affinity approaches and mass spectrometry

Journal of Peptide Science

Volumn 17, Issue 3, 2011, Pages 184-191

  Drǎguşanu, Mihaela ^a   Petre, Brînduşa Alina ^a   Przybylski, Michael ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

3 nitro tyrosine antigen antibody complex; Affinity mass spectrometry approaches; FTICR mass spectrometry; Proteolytic epitope excision; Tyrosine nitration

Indexed keywords

3 NITROTYROSINE; NITROTYROSINE ANTIBODY; PEPTIDE ANTIBODY; PEROXYNITRITE; PROSTACYCLIN SYNTHASE; TYROSINE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANTIBODY SPECIFICITY; ARTICLE; BINDING AFFINITY; BIOSENSOR; DISSOCIATION CONSTANT; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; MASS SPECTROMETRY; NITRATION; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ANTIBODIES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDES; TYROSINE;

EID: 79751471786     PISSN: 10752617     EISSN: 10991387     Source Type: Journal    
DOI: 10.1002/psc.1298     Document Type: Article

References (26)

1. Beckman JS, Ye YZ, Anderson SR, Chen J, Accavitti MA, White CR. Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry. Bio. Chem. Hoppe-Seyler 1994; 375: 81-88.
2. Cromheeke KM, Kockx MM, De Meyer GR, Bosmans JM, Bult H, Beelaerts WJ, Vrints CJ, Herman AG. Inducible nitric oxide synthase colocalizes with signs of lipid oxidation/peroxidation in human atherosclerotic plaques. Cardiovasc. Res. 1999; 43: 744-754.
3. Teunissen CE, de Vente J, Steinbusch HW. Biochemical markers related to Alzheimer's dementia in serum and cerebrospinal fluid. Neurobiol. Aging 2002; 23: 458-508.
4. Giasson BI, Duda JE, Murray IVJ, Chen Q, Souza JM, Hurtig HI, Ischiropoulos H, Trojanowski JQ, Lee VM. Oxidative damage linked to neurodegeneration by selective alpha-synuclein nitration in synucleinopathy lesions. Science 2000; 290: 985-989.
5. Beckman JS, Chen J, Crow JP, Ye YZ. Reactions of nitric oxide, superoxide and peroxynitrite with superoxide dismutase in neurodegeneration. Prog. Brain Res. 1994; 103: 371-380.
6. Beckman JS. Oxidative damage and tyrosine nitration from peroxynitrite. Chem. Res. Toxicol. 1996; 9: 836-844.
7. Ischiropoulos H. Protein tyrosine nitration-an update. Arch. Biochem. Biophys. 2009; 484: 117-121.
8. Ryberg H, Caidahl K. Chromatographic and mass spectrometric methods for quantitative determination of 3-nitrotyrosine in biological samples and their application to human samples. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2007; 851: 160-171.
9. Tsikas D. A critical review and discussion of analytical methods in the l-arginine/nitric oxide area of basic and clinical research. Anal. Biochem. 2008; 379(2): 139-163.
10. Tsikas D. Analytical methods for 3-nitrotyrosine quantification in biological samples: the unique role of tandem mass spectrometry. Amino Acids 2010; (in press), DOI: 10.1007/s00726-010-0604-5.
11. Franze T, Weller MG, Niessner R, Poschl U. Comparison of nitrotyrosine antibodies and development of immunoassays for the detection of nitrated proteins. Analyst 2004; 129: 589-596.
12. ter Steege J, Buurman W, Arends JW, Forget P. Presence of inducible nitric oxide synthase, nitrotyrosine, CD68, and CD14 in the small intestine in celiac disease. Lab. Invest. 1997; 77: 29-36.
13. Hinson JA, Michael SL, Ault SG, Pumford NR. Western blot analysis for nitrotyrosine protein adducts in livers of saline-treated and acetaminophen-treated mice. Toxicol. Sci. 2000; 53: 467-473.
14. Duda JE, Giasson BI, Chen Q, Gur TL, Hurtig HI, Stern MB, Gollomp SM, Ischiropoulos H, Lee VM, Trojanowski JQ. Widespread nitration of pathological inclusions in neurodegenerative synucleinopathies. Am. J. Pathol. 2000; 157: 1439-1445.
15. Franze T, Weller MG, Niessner R, Poschl U. Enzyme immunoassays for the investigation of protein nitration by air pollutants. Analyst 2003; 128: 824-831.
16. Petre BA, Youhnovski N, Lukkari J, Weber R, Przybylski M. Structural characterisation of tyrosine-nitrated peptides by ultraviolet and infrared matrix-assisted laser desorption/ionisation Fourier transform ion cyclotron resonance mass spectrometry. Eur. J. Mass Spectrom. 2005; 11: 513-518.
17. Schmidt P, Youhnovski N, Daiber A, Balan A, Arsic M, Bachschmid M, Przybylski M, Ullrich V. Specific nitration at Tyrosine-430 revealed by high resolution mass spectrometry as basis for redox regulation of bovine prostacyclin synthase. J. Biol. Chem. 2003; 278: 12813-12819.
18. Stefanescu R, Iacob RE, Damoc EN, Marquardt A, Amstalden E, Manea M, Perdivara I, Maftei M, Paraschiv G, Przybylski M. Mass spectrometric approaches for elucidation of antigen-antibody recognition structures in molecular immunology. Eur. J. Mass Spectrom. 2007; 13: 69-75.
19. Hager-Braun C, Tomer KB. Determination of protein-derived epitopes by mass spectrometry. Expert Rev. Proteomics 2005; 2: 745-755.
20. McLaurin J, Cecal R, Kierstadt ME, Tian X, Phinney AL, Manea M, French JE, Lambermon MH, Darabie AA, Brown ME, Janus C, Chishti MA, Horne P, Westaway D, Fraser PE, Mount HT, Przybylski M, St. George-Hyslop P. Therapeutically effective antibodies against amyloid-beta peptide target amyloid-beta residues 4-10 and inhibit cytotoxicity and fibrillogenesis. Nature Med. 2002; 8: 1263-1269.
21. Macht M, Marquardt A, Deininger SO, Damoc E, Kohlmann M, Przybylski M. "Affinity-proteomics": direct protein identification from biological material using mass spectrometric epitope mappping. Anal. Bioanal. Chem. 2004; 378: 1102-1112.
22. Ulrich M, Petre A, Youhnovski N, Promm F, Schirle M, Schumm M, Pero RS, Doyle A, Checkel J, Kita H, Thiyagarajan N, Acharya KR, Schmid-Grendelmeier P, Simon HU, Schwarz H, Tsutsui M, Shimokawa H, Bellon G, Lee JJ, Przybylski M, Doring G. Post-translational tyrosine nitration of eosinophil granule toxins mediated by eosinophil peroxidase. J. Biol. Chem. 2008; 283: 28629-28640.
23. Drǎguşanu M, Petre BA, Slǎmnoiu S, Vlad C, Tu T, Przybylski M. On-line bioaffinity-electrospray mass spectrometry for simultaneous detection, identification and quantification of protein-ligand interactions. J. Am. Soc. Mass Spectrom. 2010; 21: 1643-1648.
24. Gronewold TM. Surface acoustic wave sensors in the bioanalytical field: recent trends and challenges. Anal. Chim. Acta 2007; 603: 119-128.
25. Andra J, Bohling A, Gronewold TM, Schlecht U, Perpeet M, Gutsmann T. Surface acoustic wave biosensor as a tool to study the interaction of antimicrobial peptides with phospholipid and lipopolysaccharide model membranes. Langmuir 2008; 24: 9148-9153.
26. Petre BA, Dragusanu M, Przybylski M. Molecular recognition specificity of anti-3-nitrotyrosine antibodies revealed by affinity-mass spectrometry and immunoanalytical methods. In Applications of Mass Spectrometry in Life Safety. Popescu C, Zamfir AD, Dinca N (eds.). Springer Science: 2008; 55-67.