Sterilization, High-Level Disinfection, and Environmental Cleaning

Infectious Disease Clinics of North America

Volumn 25, Issue 1, 2011, Pages 45-76

  Rutala, William A ^a,b   Weber, David J ^a,b  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

Environmental cleaning; Healthcare associated infection; High level disinfection; Sterilization

Indexed keywords

DISINFECTANT AGENT; HYDROGEN PEROXIDE; OZONE;

ACINETOBACTER; BACTERIAL TRANSMISSION; BACTERIUM CONTAMINATION; CLEANING; CLOSTRIDIUM DIFFICILE; DISINFECTION; ENDOSCOPE; HOSPITAL INFECTION; INFECTION CONTROL; INFECTION PREVENTION; INFECTION RISK; INSTRUMENT STERILIZATION; LARYNGOSCOPE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; NOROVIRUS; REVIEW; TONOMETER; VANCOMYCIN RESISTANT ENTEROCOCCUS;

CROSS INFECTION; DISINFECTION; EQUIPMENT CONTAMINATION; HOSPITALS; HUMANS; STERILIZATION;

EID: 79551711211     PISSN: 08915520     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.idc.2010.11.009     Document Type: Review

References (221)

1. McCarthy G.M., Koval J.J., John M.A., et al. Infection control practices across Canada: do dentists follow the recommendations?. J Can Dent Assoc 1999, 65:506-511.
2. Spach D.H., Silverstein F.E., Stamm W.E. Transmission of infection by gastrointestinal endoscopy and bronchoscopy. Ann Intern Med 1993, 118:117-128.
3. Weber D.J., Rutala W.A. Lessons from outbreaks associated with bronchoscopy. Infect Control Hosp Epidemiol 2001, 22:403-408.
4. Weber D.J., Rutala W.A., DiMarino A.J. The prevention of infection following gastrointestinal endoscopy: the importance of prophylaxis and reprocessing. Gastrointestinal diseases: an endoscopic approach 2002, 87-106. Slack Inc, Thorofare (NJ). A.J. DiMarino, S.B. Benjamin (Eds.).
5. Meyers H., Brown-Elliott B.A., Moore D., et al. An outbreak of Mycobacterium chelonae infection following liposuction. Clin Infect Dis 2002, 34:1500-1507.
6. Lowry P.W., Jarvis W.R., Oberle A.D., et al. Mycobacterium chelonae causing otitis media in an ear-nose-and-throat practice. N Engl J Med 1988, 319:978-982.
7. Rutala W.A., Weber D.J. Cleaning, disinfection and sterilization. APIC text of infection control and epidemiology 2009, 21:1-21:27. Association for Professionals in Infection Control and Epidemiology, Inc, Washington, DC. R. Carrico (Ed.).
8. Rutala W.A., Weber D.J. Disinfection and sterilization in healthcare facilities. Practical handbook for healthcare epidemiologists 2010, 61-80. The University of Chicago Press, Chicago. E. Lautenbach, K. Woeltje, P.N. Malani (Eds.).
9. Spaulding E.H. Chemical disinfection of medical and surgical materials. Disinfection, sterilization, and preservation 1968, 517-531. Lea & Febiger, Philadelphia. C. Lawrence, S.S. Block (Eds.).
10. Rutala W.A., Weber D.J., Healthcare Infection Control Practices Advisory Committee Guideline for disinfection and sterilization in healthcare facilities, 2008 Available at:, Accessed December, 2010. http://www.cdc.gov/ncidod/dhqp/pdf/guidelines/Disinfection_Nov_2008.pdf.
11. Simmons B.P. CDC guidelines for the prevention and control of nosocomial infections. Guideline for hospital environmental control. Am J Infect Control 1983, 11:97-120.
12. Rutala W.A., Weber D.J. Disinfection and sterilization in health care facilities: what clinicians need to know. Clin Infect Dis 2004, 39:702-709.
13. Rutala W.A. 1994, 1995, and 1996 APIC Guidelines Committee. APIC guideline for selection and use of disinfectants. Association for Professionals in Infection Control and Epidemiology, Inc. Am J Infect Control 1996, 24:313-342.
14. Sehulster L., Chinn R.Y. Healthcare Infection Control Practices Advisory Committee. Guidelines for environmental infection control in health-care facilities. MMWR Recomm Rep 2003, 52:1-44.
15. Food and Drug Administration FDA-cleared sterilant and high-level disinfectants with general claims for processing reusable medical and dental devices-March 2009 Available at:, Accessed December, 2010. http://www.fda.gov/cdrh/ode/germlab.html.
16. Nelson D.B., Jarvis W.R., Rutala W.A., et al. Multi-society guideline for reprocessing flexible gastrointestinal endoscopes. Infect Control Hosp Epidemiol 2003, 24:532-537.
17. Gerding D.N., Peterson L.R., Vennes J.A. Cleaning and disinfection of fiberoptic endoscopes: evaluation of glutaraldehyde exposure time and forced-air drying. Gastroenterology 1982, 83:613-618.
18. Weber D.J., Rutala W.A. Environmental issues and nosocomial infections. Prevention and control of nosocomial infections 1997, 491-514. Williams and Wilkins, Baltimore (MD). R.P. Wenzel (Ed.).
19. Rutala W.A., Gergen M.F., Jones J.F., et al. Levels of microbial contamination on surgical instruments. Am J Infect Control 1998, 26:143-145.
20. Chu N.S., Chan-Myers H., Ghazanfari N., et al. Levels of naturally occurring microorganisms on surgical instruments after clinical use and after washing. Am J Infect Control 1999, 27:315-319.
21. Favero M.S. Sterility assurance: concepts for patient safety. Disinfection, sterilization and antisepsis: principles and practices in healthcare facilities 2001, 110-119. Association for Professional in Infection Control and Epidemiology, Washington, DC. W.A. Rutala (Ed.).
22. Weber D.J., Rutala W.A. Role of environmental contamination in the transmission of vancomycin-resistant enterococci. Infect Control Hosp Epidemiol 1997, 18:306-309.
23. Dufresne S., Leblond H., Chaunet M. Relationship between lumen diameter and length sterilized in the 125L ozone sterilizer. Am J Infect Control 2008, 36:291-297.
24. Bradley C.R., Babb J.R. Endoscope decontamination: automated vs. manual. J Hosp Infect 1995, 30:537-542.
25. Muscarella L.F. Advantages and limitations of automatic flexible endoscope reprocessors. Am J Infect Control 1996, 24:304-309.
26. Muscarella L.F. Automatic flexible endoscope reprocessors. Gastrointest Endosc Clin N Am 2000, 10:245-257.
27. Kircheis U., Martiny H. Comparison of the cleaning and disinfecting efficacy of four washer-disinfectors for flexible endoscopes. J Hosp Infect 2007, 66:255-261.
28. Vickery K., Ngo Q.D., Zou J., et al. The effect of multiple cycles of contamination, detergent washing, and disinfection on the development of biofilm in endoscope tubing. Am J Infect Control 2009, 37:470-475.
29. Alvarado C.J., Stolz S.M., Maki D.G. Nosocomial infections from contaminated endoscopes: a flawed automated endoscope washer. An investigation using molecular epidemiology. Am J Med 1991, 91:272S-280S.
30. Fraser V.J., Jones M., Murray P.R., et al. Contamination of flexible fiberoptic bronchoscopes with Mycobacterium chelonae linked to an automated bronchoscope disinfection machine. Am Rev Respir Dis 1992, 145:853-855.
31. Cooke R.P., Whymant-Morris A., Umasankar R.S., et al. Bacteria-free water for automatic washer-disinfectors: an impossible dream?. J Hosp Infect 1998, 39:63-65.
32. Muscarella L.F. Deja vu. All over again? The importance of instrument drying. Infect Control Hosp Epidemiol 2000, 21:628-629.
33. Rutala W.A., Weber D.J. Importance of lumen flow in liquid chemical sterilization. Am J Infect Control 1999, 20:458-459.
34. Lynch D.A., Porter C., Murphy L., et al. Evaluation of four commercial automatic endoscope washing machines. Endoscopy 1992, 24:766-770.
35. Bond W.W. Disinfection and endoscopy: microbial considerations. J Gastroenterol Hepatol 1991, 6:31-36.
36. Bond W.W. Endoscope reprocessing: problems and solutions. Disinfection, sterilization, and antisepsis in healthcare 1998, 151-163. Polyscience Publications, Champlain (NY). W.A. Rutala (Ed.).
37. Petersen B.T., Adler D.G., Chand B., American Society of Gastrointestinal Endoscopists Technology Committee, et al. Automated endoscope reprocessors. Gastrointest Endosc 2009, 69:771-776.
38. Alfa M.J., Olson N., Degagne P. Automated washing with the Reliance endoscope processing system and its equivalence to optimal manual cleaning. Am J Infect Control 2006, 34:561-570.
39. Omidbakhsh N. A new peroxide-based flexible endoscope-compatible high-level disinfectant. Am J Infect Control 2006, 34:571-577.
40. Schembre D.B. Infectious complications associated with gastrointestinal endoscopy. Gastrointest Endosc Clin N Am 2000, 10:215-232.
41. Nelson D.B. Infectious disease complications of GI endoscopy: part II, exogenous infections. Gastrointest Endosc 2003, 57:695-711.
42. Mehta A.C., Prakash U.B., Garland R., et al. Prevention of flexible bronchoscopy-associated infection. Chest 2006, 128:1742-1755.
43. Chu N.S., Favero M. The microbial flora of the gastrointestinal tract and the cleaning of flexible endoscopes. Gastrointest Endosc Clin N Am 2000, 10:233-244.
44. Alfa M.J., Sitter D.L. In-hospital evaluation of orthophthalaldehyde as a high level disinfectant for flexible endoscopes. J Hosp Infect 1994, 26:15-26.
45. Vesley D., Melson J., Stanley P. Microbial bioburden in endoscope reprocessing and an in-use evaluation of the high-level disinfection capabilities of Cidex PA. Gastroenterol Nurs 1999, 22:63-68.
46. Chu N.S., McAlister D., Antonoplos P.A. Natural bioburden levels detected on flexible gastrointestinal endoscopes after clinical use and manual cleaning. Gastrointest Endosc 1998, 48:137-142.
47. Rutala W.A., Weber D.J. FDA labeling requirements for disinfection of endoscopes: a counterpoint. Infect Control Hosp Epidemiol 1995, 16:231-235.
48. Rutala W.A., Weber D.J. Reprocessing endoscopes: United States perspective. J Hosp Infect 2004, 56:S27-S39.
49. Hanson P.J., Gor D., Clarke J.R., et al. Contamination of endoscopes used in AIDS patients. Lancet 1989, 2:86-88.
50. Hanson P.J., Gor D., Clarke J.R., et al. Recovery of the human immunodeficiency virus from fibreoptic bronchoscopes. Thorax 1991, 46:410-412.
51. Chaufour X., Deva A.K., Vickery K., et al. Evaluation of disinfection and sterilization of reusable angioscopes with the duck hepatitis B model. J Vasc Surg 1999, 30:277-282.
52. Rutala W.A., Weber D.J. Disinfection of endoscopes: review of new chemical sterilants used for high-level disinfection. Infect Control Hosp Epidemiol 1999, 20:69-76.
53. Cheung R.J., Ortiz D., DiMarino A.J. GI endoscopic reprocessing practices in the United States. Gastrointest Endosc 1999, 50:362-368.
54. Food and Drug Administration Content and format of premarket notification [510(k)] submissions for liquid chemical sterilants/high level disinfectants Available at:, Accessed December, 2010. http://www.fda.gov/cdrh/ode/397.
55. Urayama S., Kozarek R.A., Sumida S., et al. Mycobacteria and glutaraldehyde: is high-level disinfection of endoscopes possible?. Gastrointest Endosc 1996, 43:451-456.
56. Jackson J., Leggett J.E., Wilson D.A., et al. Mycobacterium gordonae in fiberoptic bronchoscopes. Am J Infect Control 1996, 24:19-23.
57. Lee R.M., Kozarek R.A., Sumida S.E., et al. Risk of contamination of sterile biopsy forceps in disinfected endoscopes. Gastrointest Endosc 1998, 47:377-381.
58. Martiny H., Floss H., Zuhlsdorf B. The importance of cleaning for the overall results of processing endoscopes. J Hosp Infect 2004, 56:S16-S22.
59. Alvarado C.J., Reichelderfer M. APIC guideline for infection prevention and control in flexible endoscopy. Association for Professionals in Infection Control. Am J Infect Control 2000, 28:138-155.
60. Society of Gastroenterology Nurses and Associates Guideline for the use of high-level disinfectants and sterilants for reprocessing of flexible gastrointestinal endoscopes. Gastroenterol Nurs 2000, 23:180-187.
61. Society of Gastroenterology Nurses and Associates Standards of infection control in reprocessing of flexible gastrointestinal endoscopes. Gastroenterol Nurs 2006, 29:142-148.
62. Society of Gastroenterology Nurses and Associates Standards for infection control and reprocessing of flexible gastrointestinal endoscopes. Gastroenterol Nurs 2000, 23:172-179.
63. American Society for Gastrointestinal Endoscopy Position statement: reprocessing of flexible gastrointestinal endoscopes. Gastrointest Endosc 1996, 43:541-546.
64. Rutala W.A. APIC guideline for selection and use of disinfectants. Am J Infect Control 1990, 18:99-117.
65. Martin M.A., Reichelderfer M. 1991, and 1993 APIC Guidelines Committee. APIC guidelines for infection prevention and control in flexible endoscopy. Am J Infect Control 1994, 22:19-38.
66. Rey J.F., Halfon P., Feryn J.M., et al. Risk of transmission of hepatitis C virus by digestive endoscopy. Gastroenterol Clin Biol 1995, 19:346-349.
67. Cronmiller J.R., Nelson D.K., Jackson D.K., et al. Efficacy of conventional endoscopic disinfection and sterilization methods against Helicobacter pylori contamination. Helicobacter 1999, 4:198-203.
68. Sartor C., Charrel R.N., de Lamballerie X., et al. Evaluation of a disinfection procedure for hysteroscopes contaminated by hepatitis C virus. Infect Control Hosp Epidemiol 1999, 20:434-436.
69. Hanson P.J., Chadwick M.V., Gaya H., et al. A study of glutaraldehyde disinfection of fibreoptic bronchoscopes experimentally contaminated with Mycobacterium tuberculosis. J Hosp Infect 1992, 22:137-142.
70. Kinney T.P., Kozarek R.A., Raltz S., et al. Contamination of single-use biopsy forceps: a prospective in vitro analysis. Gastrointest Endosc 2002, 56:209-212.
71. Best M., Springthorpe V.S., Sattar S.A. Feasibility of a combined carrier test for disinfectants: studies with a mixture of five types of microorganisms. Am J Infect Control 1994, 22:152-162.
72. Leong D, Dorsey G, Klapp M. Dilution of glutaraldehyde by automatic endoscope machine washers: the need for a quality control program. Abstracts of the 14th Annual Educational Conference of Association for Practitioners in Infection Control. Washington, DC: Association for Professionals in Infection Control and Epidemiology; 1987:108. p.130.
73. Mbithi J.N., Springthorpe V.S., Sattar S.A., et al. Bactericidal, virucidal, and mycobactericidal activities of reused alkaline glutaraldehyde in an endoscopy unit. J Clin Microbiol 1993, 31:2988-2995.
74. Kleier D.J., Averbach R.E. Glutaraldehyde nonbiologic monitors. Infect Control Hosp Epidemiol 1990, 11:439-441.
75. Cole E.C., Rutala W.A., Nessen L., et al. Effect of methodology, dilution, and exposure time on the tuberculocidal activity of glutaraldehyde-based disinfectants. Appl Environ Microbiol 1990, 56:1813-1817.
76. Collins F.M., Montalbine V. Mycobactericidal activity of glutaraldehyde solutions. J Clin Microbiol 1976, 4:408-412.
77. Masferrer R., Marquez R. Comparison of two activated glutaraldehyde solutions: Cidex Solution and Sonacide. Respir Care 1977, 22:257-262.
78. Kleier D.J., Tucker J.E., Averbach R.E. Clinical evaluation of glutaraldehyde nonbiologic monitors. Quintessence Int 1989, 20:271-277.
79. Overton D., Burgess J.O., Beck B., et al. Glutaraldehyde test kits: evaluation for accuracy and range. Gen Dent 1989, 37:126. 128.
80. Cooke R.P., Goddard S.V., Chatterley R., et al. Monitoring glutaraldehyde dilution in automated washer/disinfectors. J Hosp Infect 2001, 48:242-246.
81. Merighi A., Contato E., Scagliarini R., et al. Quality improvement in gastrointestinal endoscopy: microbiologic surveillance of disinfection. Gastrointest Endosc 1996, 43:457-462.
82. Deva A.K., Vickery K., Zou J., et al. Establishment of an in-use testing method for evaluating disinfection of surgical instruments using the duck hepatitis B model. J Hosp Infect 1996, 33:119-130.
83. Hanson P.J., Gor D., Jeffries D.J., et al. Elimination of high titre HIV from fibreoptic endoscopes. Gut 1990, 31:657-659.
84. Wu M.S., Wang J.T., Yang J.C., et al. Effective reduction of Helicobacter pylori infection after upper gastrointestinal endoscopy by mechanical washing of the endoscope. Hepatogastroenterology 1996, 43:1660-1664.
85. Kruse A., Rey J.F. Guidelines on cleaning and disinfection in GI endoscopy. Update 1999. The European Society of Gastrointestinal Endoscopy. Endoscopy 2000, 32:77-80.
86. British Thoracic Society British Thoracic Society guidelines on diagnostic flexible bronchoscopy. Thorax 2001, 56:1-21.
87. Association of Operating Room Nurses Recommended practices for use and care of endoscopes. 2000 standards, recommended practices, and guidelines 2000, AORN, Denver (CO), p. 243-7.
88. British Society of Gastroenterology Cleaning and disinfection of equipment for gastrointestinal endoscopy. Report of a working party of the British Society of Gastroenterology Endoscope Committee. Gut 1998, 42:585-593.
89. Jackson F.W., Ball M.D. Correction of deficiencies in flexible fiberoptic sigmoidoscope cleaning and disinfection technique in family practice and internal medicine offices. Arch Fam Med 1997, 6:578-582.
90. Orsi G.B., Filocamo A., Di Stefano L., et al. Italian National Survey of Digestive Endoscopy Disinfection Procedures. Endoscopy 1997, 29:732-738. [quiz: 739-40].
91. Honeybourne D., Neumann C.S. An audit of bronchoscopy practice in the United Kingdom: a survey of adherence to national guidelines. Thorax 1997, 52:709-713.
92. Michele T.M., Cronin W.A., Graham N.M., et al. Transmission of Mycobacterium tuberculosis by a fiberoptic bronchoscope. Identification by DNA fingerprinting. JAMA 1997, 278:1093-1095.
93. Bronowicki J.P., Venard V., Botte C., et al. Patient-to-patient transmission of hepatitis C virus during colonoscopy. N Engl J Med 1997, 337:237-240.
94. Agerton T., Valway S., Gore B., et al. Transmission of a highly drug-resistant strain (strain W1) of Mycobacterium tuberculosis. Community outbreak and nosocomial transmission via a contaminated bronchoscope. JAMA 1997, 278:1073-1077.
95. Srinivasan A., Wolfenden L.L., Song X., et al. An outbreak of Pseudomonas aeruginosa infections associated with flexible bronchoscopes. N Engl J Med 2003, 348:221-227.
96. Food and Drug Administration, Centers for Disease Control and Prevention FDA and CDC public health advisory: infections from endoscopes inadequately reprocessed by an automated endoscope reprocessing system 1999, Food and Drug Administration, Rockville (MD).
97. Nelson D.B., Muscarella L.F. Current issues in endoscope reprocessing and infection control during gastrointestinal endoscopy. World J Gastroenterol 2006, 12:3953-3964.
98. Willis C. Bacteria-free endoscopy rinse water-a realistic aim?. Epidemiol Infect 2005, 134:279-284.
99. Humphreys H., McGrath H., McCormick P.A., et al. Quality of final rinse water used in washer-disinfectors for endoscopes. J Hosp Infect 2002, 51:151-153.
100. Vergis A.S., Thomson D., Pieroni P., et al. Reprocessing flexible gastrointestinal endoscopes after a period of disuse: is it necessary?. Endoscopy 2007, 39:737-739.
101. Riley R., Beanland C., Bos H. Establishing the shelf life of flexible colonoscopes. Gastroenterol Nurs 2002, 25:114-119.
102. Rejchrt S., Cermak P., Pavlatova L., et al. Bacteriologic testing of endoscopes after high-level disinfection. Gastrointest Endosc 2004, 60:76-78.
103. Leung J., Vallero R., Wilson R. Surveillance cultures to monitor quality of gastrointestinal endoscope reprocessing. Am J Gastroenterol 2003, 98(1):3-5.
104. Moses F.M., Lee J. Surveillance cultures to monitor quality of gastrointestinal endoscope reprocessing. Am J Gastroenterol 2003, 98:77-81.
105. Tunuguntla A., Sullivan M.J. Monitoring quality of flexible endoscopic disinfection by microbiologic surveillance cultures. Tenn Med 2004, 97(10):453-456.
106. Beilenhoff U., Neumann C.S., Rey J.F., et al. ESGE-ESGENA guideline for quality assurance in reprocessing: microbiological surveillance testing in endoscopy. Endoscopy 2007, 39:175-181.
107. Gillespie E.E., Kotsanas D., Stuart R.L. Microbiological monitoring of endoscopes: 5-year review. J Gastroenterol Hepatol 2008, 23:1069-1074.
108. Muscarella L.F. Application of environmental sampling to flexible endoscope reprocessing: the importance of monitoring the rinse water. Infect Control Hosp Epidemiol 2002, 23:285-289.
109. Fraser T.G., Reiner S., Malcznski M., et al. Multidrug-resistant Pseudomonas aeruginosa cholangiopancreatography: failure of routine endoscope cultures to prevent an outbreak. Infect Control Hosp Epidemiol 2004, 25:856-859.
110. Bond W.W., Hedrick E.R. Microbiological culturing of environmental and medical-device surfaces. Clinical microbiology procedures handbook, section 11, epidemiologic and infection control microbiology 1992, 11.10.1-11.10.9. American Society for Microbiology, Washington, DC. H.D. Isenberg, M.J.R. Gilchrist (Eds.).
111. Centers for Disease Control Guidelines for environmental infection control in health-care facilities, 2003. MMWR Recomm Rep 2003, 52(No RR-10):1-44.
112. Pang J., Perry P., Ross A., et al. Bacteria-free rinse water for endoscope disinfection. Gastrointest Endosc 2002, 56:402-406.
113. Widmer A.F., Frei R. Decontamination, disinfection and sterilization. Manual of clinical microbiology 2003, 77-108. American Society of Microbiology, Washington, DC. P.R. Murray, E.J. Baron, M.A. Pfaller (Eds.).
114. Buss A.J., Been M.H., Borgers R.P., et al. Endoscope disinfection and its pitfalls-requirement for retrograde surveillance cultures. Endoscopy 2008, 40:327-332.
115. Blob R., Kampf G. Test models to determine cleaning efficacy with different types of bioburden and its clinical correlation. J Hosp Infect 2004, 56(Suppl):S44-S48.
116. Obee P.C., Griffith C.J., Cooper R.A., et al. Real-time monitoring in managing the decontamination of flexible gastrointestinal endoscopes. Am J Infect Control 2005, 33:202-206.
117. Sciortino C.V., Xia E.L., Mozee A. Assessment of a novel approach to evaluate the outcome of endoscope reprocessing. Infect Control Hosp Epidemiol 2004, 25:284-290.
118. Rutala W.A., Weber D.J. How to assess disease transmission when there is a failure to follow recommended disinfection and sterilization principles. Infect Control Hosp Epidemiol 2007, 28:519-524.
119. Murphy C. Inactivated glutaraldehyde: Lessons for infection control. Am J Infect Control 1998, 26:159-160.
120. Carsauw H, Debacker N. Recall of patients after use of inactive batch of Cidex disinfection solution in Belgian hospitals, Fifth International Conference of the Hospital Infection Society. Edinburgh, September 15-18, 2002. Hospital Infections Society.
121. Rutala W.A., Clontz E.P., Weber D.J., et al. Disinfection practices for endoscopes and other semicritical items. Infect Control Hosp Epidemiol 1991, 12:282-288.
122. Weber D.J., Rutala W.A. Nosocomial ocular infections. Hospital epidemiology and infection control 1999, 287-299. Lippincott Williams & Wilkins, Philadelphia. C.G. Mayhall (Ed.).
123. Centers for Disease Control Recommendations for preventing possible transmission of human T-lymphotropic virus type III/lymphadenopathy-associated virus from tears. MMWR Morb Mortal Wkly Rep 1985, 34:533-534.
124. Rutala W.A., Peacock J.E., Gergen M.F., et al. Efficacy of hospital germicides against adenovirus 8, a common cause of epidemic keratoconjunctivitis in health care facilities. Antimicrob Agents Chemother 2006, 50:1419-1424.
125. Tyler R., Ayliffe G.A., Bradley C. Virucidal activity of disinfectants: studies with the poliovirus. J Hosp Infect 1990, 15:339-345.
126. Sattar S.A., Springthorpe V.S., Karim Y., et al. Chemical disinfection of non-porous inanimate surfaces experimentally contaminated with four human pathogenic viruses. Epidemiol Infect 1989, 102:493-505.
127. Nagington J., Sutehall G.M., Whipp P. Tonometer disinfection and viruses. Br J Ophthalmol 1983, 67:674-676.
128. Chronister C.L. Structural damage to Schiotz tonometers after disinfection with solutions. Optom Vis Sci 1997, 74:164-166.
129. Craven E.R., Butler S.L., McCulley J.P., et al. Applanation tonometer tip sterilization for adenovirus type 8. Ophthalmology 1987, 94:1538-1540.
130. Pepose J.S., Linette G., Lee S.F., et al. Disinfection of Goldmann tonometers against human immunodeficiency virus type 1. Arch Ophthalmol 1989, 107:983-985.
131. Ventura L.M., Dix R.D. Viability of herpes simplex virus type 1 on the applanation tonometer. Am J Ophthalmol 1987, 103:48-52.
132. Koo D., Bouvier B., Wesley M., et al. Epidemic keratoconjunctivitis in a university medical center ophthalmology clinic; need for re-evaluation of the design and disinfection of instruments. Infect Control Hosp Epidemiol 1989, 10:547-552.
133. Jernigan J.A., Lowry B.S., Hayden F.G., et al. Adenovirus type 8 epidemic keratoconjunctivitis in an eye clinic: risk factors and control. J Infect Dis 1993, 167:1307-1313.
134. Milki A.A., Fisch J.D. Vaginal ultrasound probe cover leakage: implications for patient care. Fertil Steril 1998, 69:409-411.
135. Storment J.M., Monga M., Blanco J.D. Ineffectiveness of latex condoms in preventing contamination of the transvaginal ultrasound transducer head. South Med J 1997, 90:206-208.
136. Fritz S., Hust M.H., Ochs C., et al. Use of a latex cover sheath for transesophageal echocardiography (TEE) instead of regular disinfection of the echoscope?. Clin Cardiol 1993, 16:737-740.
137. Hignett M., Claman P. High rates of perforation are found in endovaginal ultrasound probe covers before and after oocyte retrieval for in vitro fertilization-embryo transfer. J Assist Reprod Genet 1995, 12:606-609.
138. Amis S., Ruddy M., Kibbler C.C., et al. Assessment of condoms as probe covers for transvaginal sonography. J Clin Ultrasound 2000, 28:295-298.
139. Rooks V.J., Yancey M.K., Elg S.A., et al. Comparison of probe sheaths for endovaginal sonography. Obstet Gynecol 1996, 87:27-29.
140. Odwin C.S., Fleischer A.C., Kepple D.M., et al. Probe covers and disinfectants for transvaginal transducers. J Diagnostic Med Sonography 1990, 6:130-135.
141. Benson W.G. Exposure to glutaraldehyde. J Soc Occup Med 1984, 34:63-64.
142. Garland S.M., de Crespigny L. Prevention of infection in obstetrical and gynaecological ultrasound practice. Aust N Z J Obstet Gynaecol 1996, 36:392-395.
143. Fowler C., McCracken D. US probes: risk of cross infection and ways to reduce it-comparison of cleaning methods. Radiology 1999, 213:299-300.
144. Muradali D., Gold W.L., Phillips A., et al. Can ultrasound probes and coupling gel be a source of nosocomial infection in patients undergoing sonography? An in vivo and in vitro study. AJR Am J Roentgenol 1995, 164:1521-1524.
145. Alvarado C.J., Anderson A.G., Maki D.G. Microbiologic assessment of disposable sterile endoscopic sheaths to replace high-level disinfection in reprocessing: a prospective clinical trial with nasopharygoscopes. Am J Infect Control 2009, 37:408-413.
146. Gillespie J.L., Arnold K.E., Noble-Wang J., et al. Outbreak of Pseudomonas aeruginosa infections after transrectal ultrasound-guided prostate biopsy. Urology 2007, 69:912-914.
147. Food and Drug Administration FDA Public Health Notification: reprocessing of reusable ultrasound transducer assemblies used for biopsy procedures Available at:, Accessed November 1, 2009. http://www.fda.gov/MedicalDevices/Safety/AlertsandNotices/PublicHealthNotification/ucm062086.htm.
148. Lessa F., Tak S., DeVader S.R., et al. Risk of infections associated with improperly reprocessed transrectal ultrasound-guided prostate biopsy equipment. Infect Control Hosp Epidemiol 2008, 29:289-293.
149. Masood J., Voulgaris S., Awogu O., et al. Condom perforation during transrectal ultrasound guided (TRUS) prostate biopsies: a potential risk. Int Urol Nephrol 2007, 39:1121-1124.
150. Rutala W.A., Gergen M.F., Weber D.J. Disinfection of a probe used in ultrasound-guided prostate biopsy. Infect Control Hosp Epidemiol 2007, 28(8):916-919.
151. Muscarella L.F. Prevention of disease transmission during flexible laryngoscopy. Am J Infect Control 2007, 35:536-544.
152. Muscarella L.F. Recommendations to resolve inconsistent guidelines for the reprocessing of sheathed and unsheathed rigid laryngoscopes. Infect Control Hosp Epidemiol 2007, 28:504-507.
153. Call T.R., Auerbach F.G., Riddell S.W., et al. Nosocomial contamination of laryngoscope handles: challenging current guidelines. Anesth Analg 2009, 109:479-483.
154. Boyce J.M., Potter-Bynoe G., Chenevert C., et al. Environmental contamination due to methicillin-resistant Staphylococcus aureus: possible infection control implications. Infect Control Hosp Epidemiol 1997, 18:622-627.
155. Bonten M.J., Hayden M.J., Nathan C., et al. Epidemiology of colonisation of patients and environment with vancomycin-resistant enterococci. Lancet 1996, 348:1615-1619.
156. Muto C.A., Jernigan J.A., Ostrowsky B.E., et al. SHEA guideline for preventing nosocomial transmission of multidrug-resistant strains of Staphylococcus aureus and Enterococcus. Infect Control Hosp Epidemiol 2003, 24:362-386.
157. Griffith C.J., Cooper R.A., Gilmore J., et al. An evaluation of hospital cleaning regimes and standards. J Hosp Infect 2000, 45:19-28.
158. Falk P.S., Winnike J., Woodmansee C., et al. Outbreak of vancomycin-resistant enterococci in a burn unit. Infect Control Hosp Epidemiol 2000, 21:575-582.
159. Denton M., Wilcox M.H., Parnell P., et al. Role of environmental cleaning in controlling an outbreak of Acinetobacter baumannii on a neurosurgical intensive care unit. J Hosp Infect 2004, 56:106-110.
160. Mayfield J.L., Leet T., Miller J., et al. Environmental control to reduce transmission of Clostridium difficile. Clin Infect Dis 2000, 31:995-1000.
161. Kaatz G.W., Gitlin S.D., Schaberg D.R., et al. Acquisition of Clostridium difficile from the hospital environment. Am J Epidemiol 1988, 127:1289-1294.
162. Samore M.H., Venkataraman L., DeGirolami P.C., et al. Clinical and molecular epidemiology of sporadic and clustered cases of nosocomial Clostridium difficile diarrhea. Am J Med 1996, 100:32-40.
163. Wilcox M.H., Fawley W.N., Wigglesworth N., et al. Comparison of the effect of detergent versus hypochlorite cleaning on environmental contamination and incidence of Clostridium difficile infection. J Hosp Infect 2003, 54:109-114.
164. Evans M.R., Meldrum R., Lane W., et al. An outbreak of viral gastroenteritis following environmental contamination at a concert hall. Epidemiol Infect 2002, 129:355-360.
165. Wilde J., Van R., Pickering L., et al. Detection of rotaviruses in the day care environment by reverse transcriptase polymerase chain reaction. J Infect Dis 1992, 166:507-511.
166. Akhter J., al-Hajjar S., Myint S., et al. Viral contamination of environmental surfaces on a general paediatric ward and playroom in a major referral centre in Riyadh. Eur J Epidemiol 1995, 11:587-590.
167. Sattar S.A., Jacobsen H., Springthorpe V.S., et al. Chemical disinfection to interrupt transfer of rhinovirus type 14 from environmental surfaces to hands. Appl Environ Microbiol 1993, 59:1579-1585.
168. Ward R.L., Bernstein D.I., Knowlton D.R., et al. Prevention of surface-to-human transmission of rotaviruses by treatment with disinfectant spray. J Clin Microbiol 1991, 29:1991-1996.
169. Rutala W.A., Weber D.J. Surface disinfection: should we do it?. J Hosp Infect 2001, 48(Suppl A):S64-S68.
170. Westwood J.C., Mitchell M.A., Legace S. Hospital sanitation: the massive bacterial contamination of the wet mop. Appl Microbiol 1971, 21:693-697.
171. Whitby J.L., Rampling A. Pseudomonas aeruginosa contamination in domestic and hospital environments. Lancet 1972, 1:15-17.
172. Dharan S., Mourouga P., Copin P., et al. Routine disinfection of patients' environmental surfaces. Myth or reality?. J Hosp Infect 1999, 42:113-117.
173. Sattar S.A., Lloyd-Evans N., Springthorpe V.S., et al. Institutional outbreaks of rotavirus diarrhoea: potential role of fomites and environmental surfaces as vehicles for virus transmission. J Hyg (Lond) 1986, 96:277-289.
174. Gwaltney J.M., Hendley J.O. Transmission of experimental rhinovirus infection by contaminated surfaces. Am J Epidemiol 1982, 116:828-833.
175. Sattar S.A., Jacobsen H., Rahman H., et al. Interruption of rotavirus spread through chemical disinfection. Infect Control Hosp Epidemiol 1994, 15:751-756.
176. Ray A.J., Hoyen C.K., Taub T.F., et al. Nosocomial transmission of vancomycin-resistant enterococci from surfaces. JAMA 2002, 287:1400-1401.
177. Rutala W.A., Barbee S.L., Aguiar N.C., et al. Antimicrobial activity of home disinfectants and natural products against potential human pathogens. Infect Control Hosp Epidemiol 2000, 21:33-38.
178. Silverman J., Vazquez J.A., Sobel J.D., et al. Comparative in vitro activity of antiseptics and disinfectants versus clinical isolates of Candida species. Infect Control Hosp Epidemiol 1999, 20:676-684.
179. Best M., Sattar S.A., Springthorpe V.S., et al. Efficacies of selected disinfectants against Mycobacterium tuberculosis. J Clin Microbiol 1990, 28:2234-2239.
180. Best M., Kennedy M.E., Coates F. Efficacy of a variety of disinfectants against Listeria spp. Appl Environ Microbiol 1990, 56:377-380.
181. Springthorpe V.S., Grenier J.L., Lloyd-Evans N., et al. Chemical disinfection of human rotaviruses: efficacy of commercially-available products in suspension tests. J Hyg (Lond) 1986, 97:139-161.
182. Akamatsu T., Tabata K., Hironga M., et al. Transmission of Helicobacter pylori infection via flexible fiberoptic endoscopy. Am J Infect Control 1996, 24:396-401.
183. Resnick L., Veren K., Salahuddin S.Z., et al. Stability and inactivation of HTLV-III/LAV under clinical and laboratory environments. JAMA 1986, 255:1887-1891.
184. Weber D.J., Barbee S.L., Sobsey M.D., et al. The effect of blood on the antiviral activity of sodium hypochlorite, a phenolic, and a quaternary ammonium compound. Infect Control Hosp Epidemiol 1999, 20:821-827.
185. Rice E.W., Clark R.M., Johnson C.H. Chlorine inactivation of Escherichia coli O157:H7. Emerg Infect Dis 1999, 5:461-463.
186. Anderson R.L., Carr J.H., Bond W.W., et al. Susceptibility of vancomycin-resistant enterococci to environmental disinfectants. Infect Control Hosp Epidemiol 1997, 18:195-199.
187. Ayliffe G.A., Collins B.J., Lowbury E.J., et al. Ward floors and other surfaces as reservoirs of hospital infection. J Hyg (Lond) 1967, 65:515-536.
188. Palmer P.H., Yeoman D.M. A study to assess the value of disinfectants when washing ward floors. Med J Aust 1972, 2:1237-1239.
189. Centers for Disease Control and Prevention Preventing the spread of vancomycin resistance-report from the Hospital Infection Control Practices Advisory Committee. Fed Regist 1994, 59:25758-25763.
190. Ayliffe G.A., Collins B.J., Lowbury E.J. Cleaning and disinfection of hospital floors. BMJ 1966, 5511:442-445.
191. Scott E., Bloomfield S.F. The survival and transfer of microbial contamination via cloths, hand and utensils. J Appl Bacteriol 1990, 68:271-278.
192. Rutala W.A., Cole E.C. Antiseptics and disinfectants-safe and effective?. Infect Control 1984, 5:215-218.
193. Rutala W.A., Cole E.C., Thomann C.A., et al. Stability and bactericidal activity of chlorine solutions. Infect Control Hosp Epidemiol 1998, 19:323-327.
194. Russell A.D., McDonnell G. Concentration: a major factor in studying biocidal action. J Hosp Infect 2000, 44:1-3.
195. Neely A.N. A survey of gram-negative bacteria survival on hospital fabrics and plastics. J Burn Care Rehabil 2000, 21:523-527.
196. Ayliffe G.A., Collins D.M., Lowbury E.J. Cleaning and disinfection of hospital floors. Br Med J 1966, 2:442-445.
197. Scott E., Bloomfield S.F. Investigations of the effectiveness of detergent washing, drying and chemical disinfection on contamination of cleaning cloths. J Appl Bacteriol 1990, 68:279-283.
198. Weber D.J., Rutala W.A. Occupational risks associated with the use of selected disinfectants and sterilants. Disinfection, sterilization, and antisepsis in healthcare 1998, 211-226. Polyscience Publications, Champlain (NY). W.A. Rutala (Ed.).
199. Wysowski D.K., Flynt J.W., Goldfield M., et al. Epidemic neonatal hyperbilirubinemia and use of a phenolic disinfectant detergent. Pediatrics 1978, 61:165-170.
200. Doan H.M., Keith L., Shennan A.T. Phenol and neonatal jaundice. Pediatrics 1979, 64:324-325.
201. Occupational Safety and Health Administration Occupational exposure to bloodborne pathogens; final rule. Fed Regist 1991, 56:64003-64182.
202. Occupational Safety and Health Administration. OSHA Memorandum from Stephen Mallinger. EPA-registered disinfectants for HIV/HBV. Washington, DC: Occupational Health and Safety Administration; 1997.
203. Environmental Protection Agency Pesticides: regulating pesticides Available at:, Accessed December, 2010. http://www.epa.gov/oppad001/chemregindex.htm.
204. Chitnis V., Chitnis S., Patil S., et al. Practical limitations of disinfection of body fluid spills with 10,000 ppm sodium hypochlorite (NaOCl). Am J Infect Control 2004, 32:306-308.
205. Carling P.C., Briggs J.L., Perkins J., et al. Improved cleaning of patient rooms using a new targeting method. Clin Infect Dis 2006, 42:385-388.
206. Carling P.C., Parry M.F., Rupp M.E., et al. Improving cleaning of the environment surrounding patients in 36 acute care hospitals. Infect Control Hosp Epidemiol 2008, 29:1035-1041.
207. Carling P.C., Parry M.F., Von Beheren S.M., et al. Identifying opportunities to enhance environmental cleaning in 23 acute care hospitals. Infect Control Hosp Epidemiol 2008, 29:1-7.
208. Huang S.S., Datta R., Platt R. Risk of acquiring antibiotic-resistant bacteria from prior room occupants. Arch Intern Med 2006, 166:1945-1951.
209. Drees M., Snydman D.R., Schmid C.H., et al. Prior environmental contamination increases the risk of acquisition of vancomycin-resistant enterococci. Clin Infect Dis 2008, 46:678-685.
210. Shaughnessy M, Micielli R, Depestel D, et al. Evaluation of hospital room assignment and acquisition of Clostridium difficile associated diarrhea (CDAD), 48th Annual Interscience Conference on Antimicrobial Agents and Chemotherapy and the Infections Disease Society of America. Washington, DC, Abstract K-4194, 2008.
211. Boyce J.M., Havill N.L., Otter J.A., et al. Impact of hydrogen peroxide vapor room decontamination on Clostridium difficile environmental contamination and transmission in a healthcare setting. Infect Control Hosp Epidemiol 2008, 29:723-729.
212. French G.L., Otter J.A., Shannon K.P., et al. Tackling contamination of the hospital environment by methicillin-resistant Staphylococcus aureus (MRSA): a comparison between conventional terminal cleaning and hydrogen peroxide vapour decontamination. J Hosp Infect 2004, 57:31-37.
213. Bartels M.D., Kristofferson K., Slotsbjerg T., et al. Environmental methicillin-resistant Staphylococcus aureus (MRSA) disinfection using dry-mist-generated hydrogen peroxide. J Hosp Infect 2008, 70:35-41.
214. Hall L., Otter J.A., Chewins J., et al. Use of hydrogen peroxide vapor for deactivation of Mycobacterium tuberculosis in a biological safety cabinet and a room. J Clin Microbiol 2007, 45:810-815.
215. Hardy K.J., Gossain S., Henderson N., et al. Rapid recontamination with MRSA of the environment of an intensive care unit after decontamination with hydrogen peroxide vapour. J Hosp Infect 2007, 66:360-368.
216. Johnston M.D., Lawson S., Otter J.A. Evaluation of hydrogen peroxide vapour as a method for the decontamination of surfaces contaminated with Clostridium botulinum spores. J Microbiol Methods 2005, 60:403-411.
217. Heckert R.A., Best M., Jordan L.T., et al. Efficacy of vaporized hydrogen peroxide against exotic animal viruses. Appl Environ Microbiol 1997, 63:3916-3918.
218. Klapes N.A., Vesley D. Vapor-phase hydrogen peroxide as a surface decontaminant and sterilant. Appl Environ Microbiol 1990, 56:503-506.
219. Bates C.J., Pearse R. Use of hydrogen peroxide vapour for environmental control during a Serratia outbreak in a neonatal intensive care unit. J Hosp Infect 2005, 61:364-366.
220. Shapey S., Machin K., Levi K., et al. Activity of a dry mist hydrogen peroxide system against environmental Clostridium difficile contamination in elderly care wards. J Hosp Infect 2008, 70:136-141.
221. Owens M.U., Deal D.R., Shoemaker M.O., et al. High-dose ultraviolet C light inactivates spores of Bacillus subtilis var. niger and Bacillus anthracis Sterne on non-reflective surfaces. Appl Biosaf 2005, November:1-6. J Am Biological Safety Assoc.