Readily available tissue-engineered vascular grafts

Science Translational Medicine

Volumn 3, Issue 68, 2011, Pages

  Dahl, Shannon L M ^a   Kypson, Alan P ^b   Lawson, Jeffrey H ^c   Blum, Juliana L ^a   Strader, Justin T ^a   Li, Yuling ^a   Manson, Roberto J ^c   Tente, William E ^a   DiBernardo, Louis ^c   Hensley, M Taylor ^a   Carter, Riley ^a   Williams, Tiare P ^a   Prichard, Heather L ^a   Dey, Margaret S ^a   Begelman, Keith G ^d   Niklason, Laura E ^e  

^a Humacyte Inc   (United States)

^b EAST CAROLINA UNIVERSITY   (United States)

^c DUKE UNIVERSITY MEDICAL CENTER   (United States)

^d Eastern Radiologists Inc   (United States)

^e YALE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DETERGENT; POLYGLYCOLIC ACID; TISSUE SCAFFOLD; BIOMATERIAL;

ADOLESCENT; ADULT; ALLOGRAFT; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTERIOVENOUS FISTULA; ARTERY INTIMA PROLIFERATION; ARTICLE; BABOON; BLOOD VESSEL CALCIFICATION; BLOOD VESSEL GRAFT; CONTROLLED STUDY; CORONARY ARTERY BYPASS GRAFT; DOG; EXPERIMENTAL MODEL; FEMALE; HEMODIALYSIS; HUMAN; HUMAN CELL; IMMUNOGENICITY; MALE; NONHUMAN; PRIORITY JOURNAL; SMOOTH MUSCLE FIBER; STORAGE TEMPERATURE; THERMOSTABILITY; TISSUE ENGINEERING; VASCULAR ACCESS; VASCULAR PATENCY; VASODILATATION; ANIMAL; BLOOD VESSEL PROSTHESIS; CADAVER; CELL CULTURE; CYTOLOGY; EVALUATION; MATERIALS TESTING; MECHANICAL STRESS; METABOLISM; METHODOLOGY; MIDDLE AGED; PAPIO ANUBIS; VASCULAR SMOOTH MUSCLE;

ADOLESCENT; ADULT; ANIMALS; BIOCOMPATIBLE MATERIALS; BLOOD VESSEL PROSTHESIS; CADAVER; CELLS, CULTURED; DOGS; HUMANS; MALE; MATERIALS TESTING; MIDDLE AGED; MUSCLE, SMOOTH, VASCULAR; MYOCYTES, SMOOTH MUSCLE; PAPIO ANUBIS; STRESS, MECHANICAL; TISSUE ENGINEERING; TISSUE SCAFFOLDS; VASCULAR GRAFTING; YOUNG ADULT;

EID: 79551710781     PISSN: 19466234     EISSN: 19466242     Source Type: Journal    
DOI: 10.1126/scitranslmed.3001426     Document Type: Article

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50. Acknowledgments: We thank J. Vaughn for technical assistance with graft production, T. Williams for ultrasound examinations, S. Gage for assistance during baboon implants, and CryoLife for tissue procurement. Funding: Humacyte Inc. supported the work conducted at Humacyte, Duke University, and East Carolina University. Humacyte received funding from the North Carolina Biotechnology Center. Author contributions: S.L.M.D. conducted the study design and participated in the acquisition and interpretation of all data and writing of the manuscript; A.P.K. conducted canine implants and design of canine studies, review of canine TEVG patency with angiography, CT, and writing of the manuscript; J.H.L. did the surgical implants, surgical model design, and animal study designs; J.L.B. carried out development of human grafts and monitoring of the baboon study; J.T.S. performed development of bioreactors for TEVG growth and endothelialization, culture of cells and TEVGs, mechanical testing, and assistance with T cell isolation and culture; Y.L. developed methods for EC culture and attachment and T cell proliferation assay and did immunostaining; R.J.M. measured flow rates, performed angiography, and explanted TEVGs in the baboon model; W.E.T. participated in baboon studies, protocol generation, and post-explant analysis; L.D. conducted gross and microscopic pathology; M.T.H. processed all histology; R.C. cultured human TEVGs; T.P.W. cultured canine TEVGs; H.L.P. did the histological immunostaining; M.S.D. measured DNA and assisted with T cell isolation and culture; K.G.B. performed CT angiographic image acquisition and interpretation; L.E.N. conducted the study design, interpretation of the data, and writing of themanuscript. Competing interests: S.L.M.D., J.L.B., J.T.S., Y.L., W.E.T., M.T.H., R.C., T.P.W., H.L.P., and M.S.D. are employed by Humacyte Inc. J.H.L. has stock options in Humacyte and has received honoraria for advising Humacyte. R.J.M. received fees for service consulting for Humacyte. L.D. consulted on a fee basis for Humacyte. L.E.N. owns stock in Humacyte. A.P.K. and K.G.B. report that they have no competing interests. Accession numbers: Part of this work is included in a patent application (serial number 61/430,381).