Quantitative assessment of DNA damage directly in lens epithelial cells from senile cataract patients

Molecular Vision

Volumn 17, Issue , 2011, Pages 1-6

  Sorte, K ^a   Sune, P ^a   Bhake, A ^a   Shivkumar, V B ^b   Gangane, N ^b   Basak, A ^a  

^a DATTA MEGHE INSTITUTE OF MEDICAL SCIENCES   (India)

^b MAHATMA GANDHI INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; CELL NUCLEUS MEMBRANE; CLINICAL ARTICLE; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; HUMAN; HUMAN CELL; LENS EPITHELIUM; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SCORING SYSTEM; SENILE CATARACT; SUBCAPSULAR CATARACT;

AGED; AGED, 80 AND OVER; ALZHEIMER DISEASE; CATARACT; COMET ASSAY; DNA DAMAGE; EPITHELIAL CELLS; HUMANS; LENS, CRYSTALLINE; LYMPHOCYTES; MIDDLE AGED; OXIDATIVE STRESS; REACTIVE OXYGEN SPECIES; SOFTWARE;

EID: 79551691748     PISSN: None     EISSN: 10900535     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Khurana AK, Khurana I. Anatomy and physiology of the eye. 2th ed. Delhi (India): CBS Publication & Distributors; 2007.
2. Spector A. Oxidative stress induced cataract: Mechanism of action. FASEB J 1995; 9:1173-82. [PMID: 7672510]
3. Wang X, Simpkins JW, Dykens JA, Cammarata PR. Oxidative damage to human lens epithelial cells in culture: estrogen protection of mitochondrial potential, ATP and cell viability. Invest Ophthalmol Vis Sci 2003; 44:2067-2075. [PMID:12714645]
4. Ortwerth BJ, Coots A, James HL, Linetsky M. UVA irradiation of human lens proteins produces residual oxidation ofascorbic acid even in the presence of high levels of glutathione. Arch Biochem Biophys 1998; 351:189-196. [PMID: 9515056]
5. Jose JG, Yeilding KL. Unscheduled DNA synthesis in lens epithelium following ultraviolet radiation. Exp Eye Res 1977; 24:113-119. [PMID: 851489]
6. Kojima M. Regional enzymatic analysis of UV-B and streptozotocin induced diabetic cataract lens. Lens Eye Toxic Res 1990; 7:547-561. [PMID: 1966039]
7. Kuck JF. Effect of long wave ultraviolet light on the lens. Ophthalmic Res 1983; 15:216-219. [PMID: 6634057]
8. Sinclair AJ, Barnett AH, Lunec J. Free radicals and antioxidant systems in health and disease. Br J Hosp Med 1990; 43:334-44. [PMID: 2194616]
9. Machlin LJ, Bendich A. Free radical tissue damage: protective role of antioxidant nutrients. FASEB J 1987; 1:441-445. [PMID:3315807]
10. Taarnhoj NC, Shenl AC, Gebhard DF, Brees BD, Somps CJ. Investigating markers of DNA oxidation, lipid peroxidation and stress response in Human lens epithelial cell lines. Invest Ophthalmol Vis Sci 2005; 46:3850-3854. [PMID: 16186373]
11. Wolf N, Pendergrass W, Singh N, Swisshelm K, Schwartz J. Radiation cataract: mechanism involved in their long delayed occurrence but then rapid progression. Mol Vis 2008; 14:274-285. [PMID: 18334943]
12. Kleiman NJ, Spector A. DNA single strands break in human lens epithelial cells from patients with cataract. Curr Eye Res 1993; 12:423-431. [PMID: 8344066]
13. Wolf N, Pendergrass W, Singh N, Swisshelm K, Schwartz J. Radiation cataract: mechanism involved in their long delayed occurrence but then rapid progression. Mol Vis 2008; 14:274-285. [PMID: 18334943]
14. Yao K, Wu W, Yu Y, Zeng Q, He J, Lu D, Wang K. Effect of superimposed electromagnetic noise on DNA damage of lens epithelial cells induced by microwave radiation. Invest Ophthalmol Vis Sci 2008; 49:2009-2015. [PMID: 18436834]
15. Gajjar D, Patel D, Alapure B, Praveen MR, Patel A, Johar K, Vasavada AR. Rapid action of oestradiol against hydrogen peroxide induced oxidative stress in cataractous lens epithelium: an in vitro study. Eye (Lond) 2008; 23:1-8. [PMID: 18806765]
16. Nanavaty MA, Johar K, Sivasankaran M, Vasavada A, Praveen M, Zetterström C. Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract. J Cataract Refract Surg 2006; 32:1483-1488. [PMID: 16931259]
17. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low level of DNA damage in individual cell. Exp Cell Res 1988; 175:184-191. [PMID:3345800]
18. Li W-C, Kuszak JR, Dunn K, Wang R-R, Ma W, Wang G-M, Spector A, Leib M, Cotliar AM, Weiss M, Espy J, Howard G, Farris RL, Auran J, Donn A, Hofeldt A, Mackay C, Merriam J, Mitti R, Smith TR. Lens epithelial cell apoptosis appears to be a common cellular basis for non congenital cataract development in humans and animals. J Cell Biol 1995; 130:169-181. [PMID: 7790371]
19. Spector A, Roy D. Disulfide-linked high molecular weight protein associated with human cataract. Proc Natl Acad Sci USA 1978; 75:3244-3248. [PMID: 277922]
20. Hightower KR, Reddan JR, Dziedzic DC. Susceptibility of lens epithelial membrane -SH groups to hydrogen peroxide. Invest Ophthalmol Vis Sci 1989; 30:569-574. [PMID: 2538404]
21. Bhuyan KC, Bhuyan DK. Molecular mechanism of cataracteogensis III. Toxic metabolites of oxygen as initiator of lipid peroxidation and cataract. Curr Eye Res 1984; 3:67-81. [PMID: 6317286]
22. Babizhayev MA, Deyev AI, Linberg LF. Lipid peroxidation as a possible cause of cataract. Mech Ageing Dev 1988; 44:69-89. [PMID: 3205065]
23. Moor AN, Flynn JM, Gottipati S, Giblin FJ, Cammarata PR. 17-β estradiol stimulates MAPK signaling pathway in human lens epithelial cell cultures preventing collapse of mitochondrial membrane potential during acute oxidative stress. Mitochondrion 2005; 5:235-247. [PMID: 16050986]