Detection of three virulence genes alt, ahp and aerA in Aeromonas hydrophila and their relationship with actual virulence to zebrafish

Journal of Applied Microbiology

Volumn 110, Issue 3, 2011, Pages 823-830

  Li, J ^a   Ni, X D ^a   Liu, Y J ^a   Lu, C P ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

Aerolysin (aerA); Aeromonas hydrophila; Cytotoxic enterotoxin (alt); Serine protease (ahp); Virulence properties

Indexed keywords

AMINO ACIDS; ENZYME ACTIVITY; GENES; HIERARCHICAL SYSTEMS; POLYMERASE CHAIN REACTION;

AEROLYSIN; AEROLYSIN (AERA); AEROMONAS HYDROPHILA; CYTOTOXIC; CYTOTOXIC ENTEROTOXIN (ALT); ENTEROTOXINS; PROPERTY; SERINE PROTEASE; SERINE PROTEASE (AHP); VIRULENCE PROPERTY;

FISH;

CYTOTOXIC AGENT; ENTEROTOXIN; SERINE PROTEINASE;

BACTERIUM; COHORT ANALYSIS; ENZYME ACTIVITY; GENETIC ANALYSIS; TOXIN; VIRULENCE;

AERA GENE; AEROMONAS HYDROPHILA; AHP GENE; ALT GENE; ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; CONTROLLED STUDY; FRESHWATER ENVIRONMENT; GENE EXPRESSION; GENE FREQUENCY; HEMOLYSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN DEGRADATION; ZEBRA FISH;

AEROMONAS HYDROPHILA; ANIMALIA; DANIO RERIO;

EID: 79551660032     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2011.04944.x     Document Type: Article

References (35)

1. Albert, M.J., Ansaruzzaman, M., Talukdar, K., Chopra, A.K., Kuhn, I., Rahman, M., Faruque, A.S.G., Islam, M.S. et al. (2000) Prevalence of enterotoxin genes in Aeromonas spp. isolated from children with diarrhea, healthy controls, and the environment. J Clin Microbiol 38, 3785-3790.
2. Avolio, M., La Spisa, C., Moscariello, F., De Rosa, R. and Camporese, A. (2009) Aeromonas hydrophila ecthyma gangrenosum without bacteraemia in a diabetic man: the first case report in Italy. Infez Med 17, 184-187.
3. Bi, Z.X., Liu, Y.J. and Lu, C.P. (2007) Contribution of AhyR to virulence of Aeromonas hydrophila J-1. Res Vet Sci 83, 150-156.
4. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal Biochem 72, 248-254.
5. Cahill, M.M. (1990) Virulence factors in motile Aeromonas species. J Appl Microbiol 69, 1-16.
6. Carvalho-Castro, G.A., Lopes, C.O., Leal, C.A.G., Cardoso, P.G., Leite, R.C. and Figueiredo, H.C.P. (2010) Detection of type III secretion system genes in Aeromonas hydrophila and their relationship with virulence in Nile tilapia. Vet Microbiol 144, 371-376.
7. Cascón, A., Fregenada, J., Aller, A., Yugueros, J., Temprano, C., Sánchez, M., Rodríguez-Aparicio, L. and Naharro, G. (2000a) Cloning, characterization, and insertional inactivation of a major extracellular serine protease gene with elastolytic activity from Aeromonas hydrophila. J Fish Dis 23, 49-59.
8. Cascón, A., Yugueros, J., Temprano, A., Sánchez, M., Hernanz, C., Luengo, J.M. and Naharro, G. (2000b) A major secreted elastase is essential for pathogenicity of Aeromonas hydrophila. Infect Immun 68, 3233-3241.
9. Chen, H.Q. and Lu, C.P. (1991) Study on the pathogen of epidemic septicemia occurred in cultured cyprinoid fish in southeastern China. J Nanjing Agric Univ 14, 87-91 (in Chinese).
10. Chopra, A.K., Houston, C.W., Peterson, J.W. and Jin, G.-F. (1993) Cloning, expression, and sequence analysis of a cytolytic enterotoxin gene from Aeromonas hydrophila. Can J Microbiol 39, 513-523.
11. Chopra, A.K., Pham, R. and Houston, C.W. (1994) Cloning and expression of putative cytotonic enterotoxin-encoding genes from Aeromonas hydrophila. Gene 139, 87-91.
12. Chopra, A.K., Peterson, J.W., Xu, X.J., Coppenhaver, D.H. and Houston, C.W. (1996) Molecular and biochemical characterization of a heat-labile cytotonic enterotoxin from Aeromonas hydrophila. Microb Pathog 21, 357-377.
13. Chopra, A.K., Xu, X., Ribardo, D., Gonzalez, M., Kuhl, K., Peterson, J.W. and Houston, C.W. (2000) The cytotoxic enterotoxin of Aeromonas hydrophila induces proinflammatory cytokine production and activates arachidonic acid metabolism in macrophages. Infect Immun 68, 2808-2818.
14. Daskalov, H. (2006) The importance of Aeromonas hydrophila in food safety. Food Control 17, 474-483.
15. Galindo, C.L., Sha, J., Fadl, A.A., Pillai, L.L. and Chopra, A.K. (2006) Host immune responses to Aeromonas virulence factors. Curr Immunol Rev 2, 13-26.
16. González-Serrano, C.J., Santos, J.A., García-López, M.L. and Otero, A. (2002) Virulence markers in Aeromonas hydrophila and Aeromonas veronii biovar sobria isolates from freshwater fish and from a diarrhoea case. J Appl Microbiol 93, 414-419.
17. Hänninen, M.L., Oivanen, P. and Hirvelä-Koski, V. (1997) Aeromonas species in fish, fish-eggs, shrimp and freshwater. Int J Food Microbiol 34, 17-26.
18. Heuzenroeder, M.W., Wong, C.Y. and Flower, R.L. (1999) Distribution of two hemolytic toxin genes in clinical and environmental isolates of Aeromonas spp.: correlation with virulence in a suckling mouse model. FEMS Microbiol Lett 174, 131-136.
19. Janda, J.M. and Abbott, S.L. (2010) The genus Aeromonas: taxonomy, pathogenicity, and infection. Clin Micro Rev 23, 35-73.
20. Kingombe, C.I., Huys, G., Tonolla, M., Albert, M.J., Swings, J., Peduzzi, R. and Jemmi, T. (1999) PCR detection, characterization, and distribution of virulence genes in Aeromonas spp. Appl Environ Microbiol 65, 5293-5302.
21. Murray, R.G., Dooley, J.S., Whippey, P.W. and Trust, T.J. (1988) Structure of an S layer on a pathogenic strain of Aeromonas hydrophila. J Bacteriol 170, 2625-2630.
22. Nawaz, M., Khan, S.A., Khan, A.A., Sung, K., Tran, Q., Kerdahi, K. and Steele, R. (2010) Detection and characterization of virulence genes and integrons in Aeromonas veronii isolated from catfish. Food Microbiol 27, 327-331.
23. Pablos, M., Remacha, M.A., Rodríguez-Calleja, J.M., Sant, J.A., Otero, A. and García-López, M.L. (2010) Identity, virulence genes, and clonal relatedness of Aeromonas isolates from patients with diarrhea and drinking water. Eur J Clin Microbiol Infect Dis 29, 1163-1172.
24. Pu, J.Y., Huang, X.X. and Lu, C.P. (2007) Virulence detection of Streptococcus suis type 2 in zebrafish. Sci Agric Sin 40, 2655-2658 (in Chinese).
25. Reed, L.J. and Muench, H. (1938) A simple method of estimating fifty percent end points. Am J Hyg 27, 493-497.
26. Sha, J., Kozlova, E.V. and Chopra, A.K. (2002) Role of various enterotoxins in Aeromonas hydrophila-induced gastroenteritis: generation of enterotoxin gene-deficient mutants and evaluation of their enterotoxic activity. Infect Immun 70, 1924-1935.
27. Sha, J., Erova, T.E., Alyea, R.A., Wang, S., Olano, J.P., Pancholi, V. and Chopra, A.K. (2009) Surface-expressed enolase contributes to the pathogenesis of clinical isolate SSU of Aeromonas hydrophila. J Bacteriol 191, 3095-3107.
28. Singh, V., Somvanshi, P., Rathore, G., Kapoor, D. and Mishra, B.N. (2009) Gene cloning, expression and homology modeling of hemolysin gene from Aeromonas hydrophila. Protein Expr Purif 65, 1-7.
29. Vilches, S., Urgell, C., Merino, S., Chacon, M.R., Soler, L., Castro-Escarpulli, G., Figueras, M.J. and Tomas, J.M. (2004) Complete type III secretion system of a mesophilic Aeromonas hydrophila strain. Appl Environ Microbiol 70, 6914-6919.
30. Wang, G., Clark, C.G., Liu, C., Pucknell, C., Munro, C.K., Kruk, T.M., Caldeira, R., Woodward, D.L. et al. (2003) Detection and characterization of the hemolysin genes in Aeromonas hydrophila and Aeromonas sobria by multiplex PCR. J Clin Microbiol 41, 1048-1054.
31. Wang, C.L., Cao, X., Liu, X.D., Guo, L.P., Hu, T., Ni, X.D., Liu, Y.J. and Lu, C.P. (2008) Detection of biochemical characters and extracellular proteases in the different isolates of Aeromonas hydrophila. Anim Hus Vet Med 40, 16-19 [in Chinese].
32. Wong, C.Y.F., Heuzenroeder, M.W. and Flower, R.L.P. (1998) Inactivation of two haemolytic toxin genes in Aeromonas hydrophila attenuates virulence in a suckling mouse model. Microbiology 144, 291-298.
33. Wu, C.J., Wu, J.J., Yan, J.J., Lee, H.C., Lee, N.Y., Chang, C.M., Shih, H.I., Wu, H.M. et al. (2007) Clinical significance and distribution of putative virulence markers of 116 consecutive clinical Aeromonas isolates in southern Taiwan. J Infect 54, 151-158.
34. Yu, H.B., Rao, P.S., Lee, H.C., Vilches, S., Merino, S., Tomas, J.M. and Leung, K.Y. (2004) A type III secretion system is required for Aeromonas hydrophila AH-1 pathogenesis. Infect Immun 72, 1248-1256.
35. Zhu, D.L., Li, A.H., Wang, J.G., Li, M., Cai, T.Z. and Hu, J. (2006) The correlation between the distribution pattern of virulence genes and the virulence of Aeromonas hydrophila strains. Acta Sci Nat Univ Sunyaatseni 45, 82-85 [in Chinese].