Cloning, characterization, and insertional inactivation of a major extracellular serine protease gene with elastolytic activity from Aeromonas hydrophila

Journal of Fish Diseases

Volumn 23, Issue 1, 2000, Pages 49-59

  Cascon A ^a   Fregeneda, J ^a   Aller, M ^a   Yugueros, J ^a   Temprano, A ^a   Hernanz, C ^a   Sanchez M ^a   Rodriguez Aparicio L ^a   Naharro, Germán ^a  

^a UNIVERSITY OF LEÓN   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

AHPA PROTEIN; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ENZYME INHIBITION; ENZYMIC HYDROLYSIS; EXTRACELLULAR ENZYME; GENE EXPRESSION; MOLECULAR CLONING; MOLECULAR WEIGHT; MUTANT; OPEN READING FRAME; PH; PLASMID VECTOR; SEQUENCE HOMOLOGY; SERINE PROTEINASE; SIGNAL PEPTIDE;

AEROMONAS HYDROPHILA; AEROMONAS SALMONICIDA; ESCHERICHIA COLI;

EID: 0034112591     PISSN: 01407775     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2761.2000.00206.x     Document Type: Article

References (37)

1. Altwegg M. & Geiss H.K. (1989) Aeromonas as a human pathogen. Critical Reviews in Microbiology 16, 253-286.
2. Austin B. & Austin D.A. (1993) Bacterial Fish Pathogens, 2nd edn. Ellis Horwood, Chichester.
3. Birnboim H.C. & Doly J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7, 1513-1523.
4. Bjorn M.J., Sokol P.A. & Iglewski B.H. (1979) Influence of iron on yields of extracellular products in Pseudomonas aeruginosa cultures. Journal of Bacteriology 138, 193-200.
5. Bradford M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254.
6. Buckley J.T., Halasa L.N. & McIntyre S. (1982) Purification and partial characterization of a bacterial phospholipid:cholesterol acyltransferase. Journal of Biological Chemistry 255, 3320-3325.
7. Chakraborty T., Huhle B., Hof H., Bergbauer H. & Goebel W. (1987) Marker exchange mutagenesis of the aerolysin determinant in Aeromonas hydrophila demonstrates the role of aerolysin in A. hydrophila-associated systemic infections. Infection and Immunity 55, 2274-2280.
8. Chang T.M., Liu C.C. & Chang M.C. (1997) Cloning and sequence analysis of the gene (eprA1) encoding an extra-cellular protease. Gene 199, 225-229.
9. Coleman G. & Whitby P.W. (1993) A comparison of the amino acid sequence of the serine protease of the fish pathogen Aeromonas salmonicida subsp. salmonicida with those of subtilisin-type enzymes relative to their substrate-binding sites. Journal of General Microbiology 139, 245-249.
10. Del Corral F., Shotts E.B., Jr & Brown J. (1990) Adherence, haemagglutination, and cell surface characteristics of motile aeromonads virulent for fish. Journal of Fish Diseases 13, 255-268.
11. Dower W.J., Miller J.F. & Ragsdale C.W. (1988) High efficiency transformation of E. coli by high voltage electro-poration. Nucleic Acids Research 7, 6127-6145.
12. Drinan E.M. & Smith P.R. (1985) Histopathology of a mutant Aeromonas salmonicida infection in Atlantic salmon (Salmo salar). In: Fish and Shellfish Pathology (ed. by A.E. Ellis), pp. 79-83. Academic Press, London.
13. Eggset G., Bjornsdottir R., McQueen Leifson R., Arnesen J.A., Coucheron D.H. & Jorgensen T.O. (1994) Extracellular glycerophospholipid:cholesterol acyltransferase from Aeromonas salmonicida: activation by serine protease. Journal of Fish Diseases 17, 17-29.
14. Ellis A.E. (1997) The extracellular toxins of Aeromonas salmonicida ssp. salmonicida. In: Furunculosis. Multidisciplinary Fish Disease Research (ed. by E.M. Bernoth, A.E. Ellis, P.J. Midtlyng, G. Olivier & P. Smith), pp. 248-268. Academic Press, London.
15. Handfield M., Simard P., Couillard M. & Letarte R. (1996) Aeromonas hydrophila isolated from food and drinking water: hemagglutination, hemolysis, and cytotoxicity for a human intestinal cell line (HT-29). Applied and Environmental Microbiology 62, 3459-3461.
16. Hernanz C., Flaño E., López P., Villena A., Anguita J., Cascón A., Sánchez M., Razquín B. & Naharro G. (1998) Molecular characterization of the Aeromonas hydrophila aroA gene and potential use of an auxotrophic aroA mutant as a live attenuated vaccine. Infection and Immunity 66, 1813-1821.
17. Holmberg S.K., Schell W.L. & Ganning G.R. (1986) Aeromonas intestinal infections in the United States. Annals of Internal Medicine 150, 683-689.
18. Janda J.M. & Duffey P.S. (1988) Mesophilic aeromonads in human disease: current taxonomy, laboratory identification, and infectious disease spectrum. Reviews of Infectious Diseases 10, 980-997.
19. Laemmli U.K. (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature (London) 227, 680-685.
20. Leung K.Y. & Stevenson R.M.W. (1988a) Tn5-induced protease-deficient strains of Aeromonas hydrophila with reduced virulence for fish. Infection and Immunity 56, 2639-2644.
21. Leung K.Y. & Stevenson R.M. (1988b) Characteristics and distribution of extracellular proteases from Aeromonas hydrophila. Journal of General Microbiology 134, 151-160.
22. Loewy A.G., Santer U.V., Wieczorek M., Blodgett J.K., Jones S.W. & Cheronis J.C. (1993) Purification and characterization of a novel zinc-proteinase of Aeromonas hydrophila. Journal of Biological Chemistry 268, 9071-9078.
23. Miller J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
24. Nagahase K., Tanaka T., Hishinuma F., Kuroda M. & Sakaguchi K. (1977) Control of tryptophan synthetase amplified by varying the number of composite plasmids in E. coli cells. Gene 1, 141-152.
25. Nieto T.P., Santos Y., Rodríguez L.A. & Ellis A.E. (1991) An extracellular acetylcholinesterase produced by Aeromonas hydrophila is a major lethal toxin for fish. Microbial Pathogenesis 11, 101-110.
26. Paniagua C., Rivero. O., Anguita J. & Naharro G. (1990) Pathogenicity factors and virulence for rainbow trout (Salmo gairdneri) of motile Aeromonas spp. isolated from a river. Journal of Clinical Microbiology 28, 350-355.
27. Priefer U., Simon R. & Puyhler A. (1984) Cloning with cosmid. In: Advanced Molecular Genetics (ed. by A. Puhler & K.N. Timmis), pp. 190-201. Springer-Verlag, Berlin.
28. Reed L.J. & Muench H. (1938) A simple method of estimating fifty percent end point. American Journal of Hygiene 27, 493-497.
29. Rivero O., Anguita J., Mateos D., Paniagua C. & Naharro G. (1991) Cloning and characterization of an extracellular temperature-labile serine protease gene from Aeromonas hydrophila. FEMS Microbiology Letters 81, 1-8.
30. Rivero O., Anguita J., Paniagua C. & Naharro G. (1990) Molecular cloning and characterization of an extracellular protease gene from Aeromonas hydrophila. Journal of Bacteriology 172, 3905-3908.
31. 32: all are lethal for fish. Microbial Pathogenesis 13, 17-24.
32. Sambrook J., Fritsch E.F. & Maniatis T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
33. Simon R., Priefer U. & Pühler A. (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Biol Technology 1, 784-791.
34. Taylor R.K., Manoil C. & Mekalanos J.J. (1989) Broad-host-range vectors for delivery of TnphoA: use in genetic analysis of secreted virulence determinants of Vibrio cholerae. Journal of Bacteriology 171, 1870-1878.
35. Vipond R., Bricknell I.R., Durant E., Bowden T.J., Ellis A.E., Smith M. & McIntyre S. (1998) Defined deletion mutants demonstrate that the major secreted toxins are not essential for the virulence of Aeromonas salmonicida. Infection and Immunity 66, 1990-1998.
36. Whitby P.W., Landon M. & Coleman G. (1992) The cloning and nucleotide sequence of the serine prorease gene (aspA) of Aeromonas salmonicida ssp. salmonicida. FEMS Microbiology Letters 99, 65-72.
37. Yanisch-Perron C., Vieira J. & Messing J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.