Performance of a multiplex assay compared to enzyme and precipitation methods for anti-ENA testing in systemic lupus and systemic sclerosis

Journal of Immunological Methods

Volumn 365, Issue 1-2, 2011, Pages 126-131

  Albon, S ^a   Bunn, C ^a   Swana, G ^b   Karim, Y ^b,c  

^a ROYAL FREE HOSPITAL   (United Kingdom)

^b ST THOMAS HOSPITAL   (United Kingdom)

^c FRIMLEY PARK HOSPITAL   (United Kingdom)

Author keywords

Autoantibodies; Autoimmune; Connective tissue disease; FIDIS; Luminex; Scleroderma

Indexed keywords

CELL NUCLEUS ANTIGEN; EXTRACTABLE NUCLEAR ANTIGEN; UNCLASSIFIED DRUG;

ADDRESSABLE LASER BEAD IMMUNOASSAY; ARTICLE; CONTROLLED STUDY; COUNTER IMMUNOELECTROPHORESIS; ENZYME ASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNOASSAY; IMMUNOPRECIPITATION; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; SYSTEMIC LUPUS ERYTHEMATOSUS; SYSTEMIC SCLEROSIS;

ANTIBODIES, ANTINUCLEAR; ANTIGENS, NUCLEAR; AUTOANTIBODIES; AUTOANTIGENS; COUNTERIMMUNOELECTROPHORESIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOASSAY; LUPUS ERYTHEMATOSUS, SYSTEMIC; NUCLEAR PROTEINS; RIBONUCLEOPROTEINS; SCLERODERMA, SYSTEMIC; SNRNP CORE PROTEINS;

EID: 79551580325     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2010.12.010     Document Type: Article

References (19)

1. Avaniss-Aghajani E., Berzon S., Sarkissian A. Clinical value of multiplexed bead based immunoassays for detection of autoantibodies to nuclear antigens. Clin. Vaccine Immunol. 2007, 14:505.
2. Bernstein R.M., Bunn C.C., Hughes G.R.V. Identification of antibodies to acidic antigens by counterimmunoelectrophoresis. Ann. Rheum. Dis. 1982, 41:554.
3. Biagini R.E., Parks C.G., Smith J.P., Sammons D.L., Robertson S.A. Analytical performance of the AtheNA multi-lyte ANA II assay in sera from lupus patients with multiple positive ANAs. Anal. Bioanal. Chem. 2007, 388:613.
4. Copple S.S., Martins T.B., Masterson C., Joly E., Hill H.R. Comparison of three multiplex immunoassays for detection of antibodies to extractable antibodies using clinically defined sera. Ann. N.Y. Acad. Sci. 2007, 1109:464.
5. Desplat-Jego S., Bardin N., Larida B., Sanmarco M. Evaluation of the BioPlex 2200 ANA screen for the detection of antinuclear antibodies and comparison with conventional methods. Ann. N.Y. Acad. Sci. 2007, 1109:245.
6. Egner W. The use of laboratory tests in the diagnosis of SLE. J. Clin. Pathol. 2000, 53:424.
7. Hanly J.G., Thompson K., McCurdy G., Fougere L., Theriault C., Wilton K. Measurement of autoantibodies using multiplex methodology in patients with systemic lupus erythematosus. J. Immunol. Meth. 2010, 352:147.
8. Hanly J.G., Su L., Farewell V., Fritzler M.J. Comparison between multiplex assays for autoantibody detection in systemic lupus erythematosus. J. Immunol. Meth. 2010, 358:75.
9. Kurata N., Tan E.M. Identification of antibodies to nuclear acidic antigens by counterimmunoelectrophoresis. Arthritis Rheum. 1976, 19:574.
10. Lock R.J., Unsworth D.J. Antibodies to extractable nuclear antigens. Has technological drift affected clinical interpretation. J. Clin. Pathol. 2001, 54:187.
11. Martins T.B., Burlingame R.W., von Muhlen C.A., Jaskowski T.D., Litwin C.M., Hill H.R. Evaluation of multiplexed fluorescent microsphere immunoassay for detection of autoantibodies to nuclear antigens. Clin. Diagn. Lab. Immunol. 2004, 11:1054.
12. Nifli A.-P., Notas G., Mamoulaki M., Niniraki M., Ampartzaki V., Theodoropoulos P.A., Kopnitsky M.J., Castanas E. Comparison of a multiplex, bead based fluorescent assay and immunofluorescence methods for the detection of ANA and ANCA antibodies in human serum. J. Immunol. Meth. 2006, 311:189.
13. Orton S.M., Peace-Brewer A., Schmidt J.L., Freeman K., Miller W.C., Folds J.D. Practical evaluation of methods for the detection and specificity of autoantibodies to extractable nuclear antigens. Clin. Diagn. Lab. Immunol. 2004, 11:297.
14. Parker J.C., Burlingame R.W., Bunn C.C. Prevalence of antibodies to Ro-52 in a serologically defined population of patients with systemic sclerosis. J. Autoimmune Dis. 2009, 6:2.
15. Peene I., Meheus L., De Keyser S., Humbel R., Veys E.M., De Keyser F. Anti-Ro-52 reactivity is an independent and additional serum marker in connective tissue disease. Ann. Rheum. Dis. 2002, 61:929.
16. Phan T.G., Wong R.C.W., Adelstein S. Autoantibodies to extractable nuclear antigens: making detection and interpretation more meaningful. Clin. Diagn. Lab. Immunol. 2002, 9:1.
17. Rouquette A.M., Desgruelles C., Laroche P. Evaluation of the new multiplexed immunoassay, FIDIS, for simultaneous quantitative determination of antinuclear antibodies and comparison with conventional methods. Am. J. Clin. Pathol. 2003, 120:676.
18. Shovman O., Gilburd B., Zandman-Goddard G., Yehiely A., Langevitz P., Shoenfeld Y. Multiplexed AtheNA multi-lyte immunoassay for ANA screening in autoimmune diseases. Autoimmunity 2005, 38:105.
19. Tan E.M., Cohen A.S., Fries J.F., Masi A.T., McShane D.J., Rothfield N.F., Schaller J.G., Talal N., Winchester R.J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982, 25:1271.