Comparison of a multiplex, bead-based fluorescent assay and immunofluorescence methods for the detection of ANA and ANCA autoantibodies in human serum

Journal of Immunological Methods

Volumn 311, Issue 1-2, 2006, Pages 189-197

  Nifli, Artemissia Phoebe ^a   Notas, George ^a   Mamoulaki, Marilena ^a   Niniraki, Maria ^a   Ampartzaki, Vaso ^a   Theodoropoulos, Panayiotis A ^a   Kopnitsky, Mark J ^b   Castanas, Elias ^a  

^a UNIVERSITY OF CRETE   (Greece)

^b Zeus Scientific Inc   (United States)

Author keywords

Antineutroplil cytoplasmic antibodies (ANCA); Antinuclear antibodies (ANA); Autoimmune diseases; Extractable nuclear antigen (ENA) screen; Multiplex bead based fluorescent assay

Indexed keywords

ANTINUCLEAR ANTIBODY; CELL NUCLEUS ANTIGEN; MICROSPHERE; NEUTROPHIL CYTOPLASMIC ANTIBODY;

ANTIBODY DETECTION; ANTIBODY SCREENING; ARTICLE; AUTOIMMUNE DISEASE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNOFLUORESCENCE; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; STATISTICAL SIGNIFICANCE; TECHNOLOGY;

ANTIBODIES, ANTINEUTROPHIL CYTOPLASMIC; ANTIBODIES, ANTINUCLEAR; AUTOIMMUNE DISEASES; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HUMANS; MICROSPHERES; RETROSPECTIVE STUDIES; STATISTICS, NONPARAMETRIC;

EID: 33646104678     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2006.02.004     Document Type: Article

References (35)

1. Buliard A., Fortenfant F., Ghillani-Dalbin P., Musset L., Oksman F., and Olsson N.O. Analysis of nine autoantibodies associated with systemic autoimmune diseases using the Luminex technology. Results of a multicenter study. Ann. Biol. Clin. (Paris) 63 (2005) 51-58
2. Camilla C., Mely L., Magnan A., Casano B., Prato S., Debono S., Montero F., Defoort J.P., Martin M., and Fert V. Flow cytometric microsphere-based immunoassay: analysis of secreted cytokines in whole-blood samples from asthmatics. Clin. Diagn. Lab. Immunol. 8 (2001) 776-784
3. Carson R.T., and Vignali D.A. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J. Immunol. Methods 227 (1999) 41-52
4. Coremans I.E., Hagen E.C., van der Voort E.A., van der Woude F.J., Daha M.R., and Breedveld F.C. Autoantibodies to neutrophil cytoplasmic enzymes in Felty's syndrome. Clin. Exp. Rheumatol. 11 (1993) 255-262
5. de Jager W., Te V.H., Prakken B.J., Kuis W., and Rijkers G.T. Simultaneous detection of 15 human cytokines in a single sample of stimulated peripheral blood mononuclear cells. Clin. Diagn. Lab. Immunol. 10 (2003) 133-139
6. Dunbar S.A., Vander Zee C.A., Oliver K.G., Karem K.L., and Jacobson J.W. Quantitative, multiplexed detection of bacterial pathogens: DNA and protein applications of the Luminex LabMAP system. J. Microbiol. Methods 53 (2003) 245-252
7. Fritzler M.J., Wiik A., Tan E.M., Smolen J.S., McDougal J.S., Chan E.K., Gordon T.P., Hardin J.A., Kalden J.R., Lahita R.G., Maini R.N., Reeves W.H., Rothfield N.F., Takasaki Y., Wilson M., Byrd M.G., Slivka L., and Koziol J.A. A critical evaluation of enzyme immunoassay kits for detection of antinuclear autoantibodies of defined specificities: III. Comparative performance characteristics of academic and manufacturers' laboratories. J. Rheumatol. 30 (2003) 2374-2381
8. Froelich C.J., Wallman J., Skosey J.L., and Teodorescu M. Clinical value of an integrated ELISA system for the detection of 6 autoantibodies (ssDNA, dsDNA, Sm, RNP/Sm, SSA, and SSB). J. Rheumatol. 17 (1990) 192-200
9. Galeazzi M., Morozzi G., Sebastiani G.D., Bellisai F., Marcolongo R., Cervera R., De Ramon G.E., Fernandez-Nebro A., Houssiau F., Jedryka-Goral A., Mathieu A., Papasteriades C., Piette J.C., Scorza R., and Smolen J. Anti-neutrophil cytoplasmic antibodies in 566 European patients with systemic lupus erythematosus: prevalence, clinical associations and correlation with other autoantibodies. European concerted action on the immunogenetics of SLE. Clin. Exp. Rheumatol. 16 (1998) 541-546
10. Hoffman G.S., and Specks U. Antineutrophil cytoplasmic antibodies. Arthritis Rheum. 41 (1998) 1521-1537
11. Ignat G.P., Rat A.C., Sychra J.J., Vo J., Varga J., and Teodorescu M. Information on diagnosis and management of systemic lupus erythematosus derived from the routine measurement of 8 nuclear autoantibodies. J. Rheumatol. 30 (2003) 1761-1769
12. Jaskowski T.D., Schroder C., Martins T.B., Mouritsen C.L., Litwin C.M., and Hill H.R. Screening for antinuclear antibodies by enzyme immunoassay. Am. J. Clin. Pathol. 105 (1996) 468-473
13. Kang I., Siperstein R., Quan T., and Breitenstein M.L. Utility of age, gender, ANA titer and pattern as predictors of anti-ENA and -dsDNA antibodies. Clin. Rheumatol. 23 (2004) 509-515
14. Kavanaugh A., Tomar R., Reveille J., Solomon D.H., and Homburger H.A. Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. American College of Pathologists. Arch. Pathol. Lab. Med. 124 (2000) 71-81
15. Khan S.S., Smith M.S., Reda D., Suffredini A.F., and McCoy Jr. J.P. Multiplex bead array assays for detection of soluble cytokines: comparisons of sensitivity and quantitative values among kits from multiple manufacturers. Cytom. B. Clin. Cytom. 61 (2004) 35-39
16. Lal G., Balmer P., Joseph H., Dawson M., and Borrow R. Development and evaluation of a tetraplex flow cytometric assay for quantitation of serum antibodies to Neisseria meningitidis serogroups A, C, Y, and W-135. Clin. Diagn. Lab. Immunol. 11 (2004) 272-279
17. Lal G., Balmer P., Stanford E., Martin S., Warrington R., and Borrow R. Development and validation of a nonaplex assay for the simultaneous quantitation of antibodies to nine Streptococcus pneumoniae serotypes. J. Immunol. Methods 296 (2005) 135-147
18. Martins T.B., Burlingame R., von Muhlen C.A., Jaskowski T.D., Litwin C.M., and Hill H.R. Evaluation of multiplexed fluorescent microsphere immunoassay for detection of autoantibodies to nuclear antigens. Clin. Diagn. Lab. Immunol. 11 (2004) 1054-1059
19. Moss D.M., Montgomery J.M., Newland S.V., Priest J.W., and Lammie P.J. Detection of cryptosporidium antibodies in sera and oral fluids using multiplex bead assay. J. Parasitol. 90 (2004) 397-404
20. Phan T.G., Wong R.C., and Adelstein S. Autoantibodies to extractable nuclear antigens: making detection and interpretation more meaningful. Clin. Diagn. Lab. Immunol. 9 (2002) 1-7
21. Pickering J.W., Martins T.B., Schroder M.C., and Hill H.R. Comparison of a multiplex flow cytometric assay with enzyme-linked immunosorbent assay for quantitation of antibodies to tetanus, diphtheria, and Haemophilus influenzae Type b. Clin. Diagn. Lab. Immunol. 9 (2002) 872-876
22. Pollock W., Clarke K., Gallagher K., Hall J., Luckhurst E., McEvoy R., Melny J., Neil J., Nikoloutsopoulos A., Thompson T., Trevisin M., and Savige J. Immunofluorescent patterns produced by antineutrophil cytoplasmic antibodies (ANCA) vary depending on neutrophil substrate and conjugate. J. Clin. Pathol. 55 (2002) 680-683
23. Quinton J.F., Sendid B., Reumaux D., Duthilleul P., Cortot A., Grandbastien B., Charrier G., Targan S.R., Colombel J.F., and Poulain D. Anti-Saccharomyces cerevisiae mannan antibodies combined with antineutrophil cytoplasmic autoantibodies in inflammatory bowel disease: prevalence and diagnostic role. Gut 42 (1998) 788-791
24. Ray C.A., Bowsher R.R., Smith W.C., Devanarayan V., Willey M.B., Brandt J.T., and Dean R.A. Development, validation, and implementation of a multiplex immunoassay for the simultaneous determination of five cytokines in human serum. J. Pharm. Biomed. Anal. 36 (2005) 1037-1044
25. Savige J.A., Gallicchio M.C., Stockman A., Cunningham T.J., Rowley M.J., Georgiou T., and Davies D. Anti-neutrophil cytoplasm antibodies in rheumatoid arthritis. Clin. Exp. Immunol. 86 (1991) 92-98
26. Savige J., Nassis L., Cooper T., Paspaliaris B., Martinello P., and MacGregor D. Antineutrophil cytoplasmic antibody (ANCA)-associated systemic vasculitis after immunisation with bacterial proteins. Clin. Exp. Rheumatol. 20 (2002) 783-789
27. Savige J., Pollock W., and Trevisin M. What do antineutrophil cytoplasmic antibodies (ANCA) tell us?. Best. Pract. Res. Clin. Rheumatol. 19 (2005) 263-276
28. Saxon A., Shanahan F., Landers C., Ganz T., and Targan S. A distinct subset of antineutrophil cytoplasmic antibodies is associated with inflammatory bowel disease. J. Allergy Clin. Immunol. 86 (1990) 202-210
29. Shovman O., Gilburd B., Zandman-Goddard G., Yehiely A., Langevitz P., and Shoenfeld Y. Multiplexed AtheNA Multi-Lyte immunoassay for ANA screening in autoimmune diseases. Autoimmunity 38 (2005) 105-109
30. Tan E.M., Feltkamp T.E., Smolen J.S., Butcher B., Dawkins R., Fritzler M.J., Gordon T., Hardin J.A., Kalden J.R., Lahita R.G., Maini R.N., McDougal J.S., Rothfield N.F., Smeenk R.J., Takasaki Y., Wiik A., Wilson M.R., and Koziol J.A. Range of antinuclear antibodies in "healthy" individuals. Arthritis Rheum. 40 (1997) 1601-1611
31. Ulvestad E., Kanestrom A., Madland T.M., Thomassen E., Haga H.J., and Vollset S.E. Evaluation of diagnostic tests for antinuclear antibodies in rheumatological practice. Scand. J. Immunol. 52 (2000) 309-315
32. van der Woude F.J., Rasmussen N., Lobatto S., Wiik A., Permin H., van Es L.A., van der G.M., van der Hem G.K., and The T.H. Autoantibodies against neutrophils and monocytes: tool for diagnosis and marker of disease activity in Wegener's granulomatosis. Lancet 1 (1985) 425-429
33. Vidrich A., Lee J., James E., Cobb L., and Targan S. Segregation of pANCA antigenic recognition by DNase treatment of neutrophils: ulcerative colitis, type 1 autoimmune hepatitis, and primary sclerosing cholangitis. J. Clin. Immunol. 15 (1995) 293-299
34. Westman K.W., Selga D., Bygren P., Segelmark M., Baslund B., Wiik A., and Wieslander J. Clinical evaluation of a capture ELISA for detection of proteinase-3 antineutrophil cytoplasmic antibody. Kidney Int. 53 (1998) 1230-1236
35. Wong R.C., Silvestrini R.A., Savige J.A., Fulcher D.A., and Benson E.M. Diagnostic value of classical and atypical antineutrophil cytoplasmic antibody (ANCA) immunofluorescence patterns. J. Clin. Pathol. 52 (1999) 124-128