Assessment of cytogenetic damage and oxidative stress in personnel occupationally exposed to the pulsed microwave radiation of marine radar equipment

International Journal of Hygiene and Environmental Health

Volumn 214, Issue 1, 2011, Pages 59-65

  Garaj Vrhovac, Vera ^a   Gajski, Goran ^a   Pažanin, Senijo ^b   Šarolić, Antonio ^c   Domijan, Ana Marija ^a   Flajs, Dubravka ^a   Peraica, Maja ^a  

^a INSTITUTE FOR MEDICAL RESEARCH AND OCCUPATIONAL HEALTH   (Croatia)

^b Naval Medical Institute Croatia   (Croatia)

^c UNIVERSITY OF SPLIT   (Croatia)

Author keywords

DNA damage; Occupational exposure; Oxidative stress; Peripheral blood lymphocytes; Pulsed microwave radiation

Indexed keywords

BIOLOGICAL MARKER; CYTOTOXIN; GLUTATHIONE; MALONALDEHYDE; PLASMA PROTEIN;

ABSORPTION; ADULT; ARTICLE; BLOOD; COMET ASSAY; DNA DAMAGE; ELECTROMAGNETIC FIELD; FEMALE; HUMAN; INSTRUMENTATION; LYMPHOCYTE; MALE; MICRONUCLEUS; MICRONUCLEUS TEST; MICROWAVE RADIATION; MIDDLE AGED; OCCUPATIONAL EXPOSURE; OXIDATIVE STRESS; PHYSIOLOGY; TELECOMMUNICATION;

ABSORPTION; ADULT; BIOLOGICAL MARKERS; BLOOD PROTEINS; COMET ASSAY; CYTOTOXINS; DNA DAMAGE; ELECTROMAGNETIC FIELDS; FEMALE; GLUTATHIONE; HUMANS; LYMPHOCYTES; MALE; MALONDIALDEHYDE; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MICRONUCLEUS TESTS; MICROWAVES; MIDDLE AGED; OCCUPATIONAL EXPOSURE; OXIDATIVE STRESS; RADAR;

EID: 79551560541     PISSN: 14384639     EISSN: 1618131X     Source Type: Journal    
DOI: 10.1016/j.ijheh.2010.08.003     Document Type: Article

References (42)

1. Ahlbom A., Bridges J., de Seze R., Hillert L., Juutilainen J., Mattsson M.O., Neubauer G., Schüz J., Simko M., Bromen K. Possible effects of electromagnetic fields (EMF) on human health - opinion of the scientific committee on emerging and newly identified health risks (SCENIHR). Toxicology 2008, 246:248-250.
2. Betti C., Davini T., Giannessi L., Loprieno N., Barale R. Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects. Mutat. Res. 1994, 307:323-333.
3. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
4. Chou C.K. Thirty-five years in bioelectromagnetics research. Bioelectromagnetics 2007, 28:3-15.
5. Collins A.R., Oscoz A.A., Brunborg G., Gaivão I., Giovannelli L., Kruszewski M., Smith C.C., Stetina R. The comet assay: topical issues. Mutagenesis 2008, 23:143-151.
6. Dasdag S., Bilgin H.M., Akdag M.Z., Celik H., Aksen F. Effect of long term mobile phone exposure on oxidative-antioxidative processes and nitric oxide in rats. Biotechnol. Biotechnol. Equip. 2008, 22:992-997.
7. Drury J.A., Nycyk J.A., Cooke R.W.I. Comparison of urinary and plasma malondialdehyde in preterm infants. Clin. Chim. Acta 1997, 263:177-185.
8. Durney C.H., Massoudi H., Iskander M.F. Radiofrequency Radiation Dosimetry Handbook 1986, Brooks Air Force Base, Texas. 4th ed.
9. Ellman G.L. A colorimetric method for determining low concentrations of mercaptans. Arch. Biochem. Biophys. 1958, 74:443-450.
10. Fenech M., Chang W.P., Kirsch-Volders M., Holland N., Bonassi S., Zeiger E. HUMN project: detailed description of scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat. Res. 2003, 534:65-75.
11. Fenech M., Morley A.A. Measurement of micronuclei in lymphocytes. Mutat. Res. 1985, 147:29-36.
12. Ferreira A.R., Bonatto F., de Bittencourt Pasquali M.A., Polydoro M., Dal-Pizzol F., Fernández C., de Salles A.A., Moreira J.C. Oxidative stress effects on the central nervous system of rats after acute exposure to ultra high frequency electromagnetic fields. Bioelectromagentics 2006, 27:487-493.
13. Gajski G., Garaj-Vrhovac V., Oreščanin V. Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: standard and Fpg-modified comet assay. Toxicol. Appl. Pharmacol. 2008, 231:85-93.
14. Gajski G., Garaj-Vrhovac V. Radioprotective effects of honeybee venom (Apis mellifera) against 915-MHz microwave radiation-induced DNA damage in Wistar rat lymphocytes: in vitro study. Int. J. Toxicol. 2009, 28:88-98.
15. Garaj-Vrhovac V., Horvat D., Koren Z. The effect of microwave radiation on the cell genome. Mutat. Res. 1990, 243:87-93.
16. Garaj-Vrhovac V. Micronucleus assay and lymphocyte mitotic activity in risk assessment of occupational exposure to microwave radiation. Chemosphere 1999, 39:2301-2312.
17. Garaj-Vrhovac V., Oreščanin V. Assessment of DNA sensitivity in peripheral blood leukocytes after occupational exposure to microwave radiation: the alkaline comet assay and chromatid breakage assay. Cell Biol. Toxicol. 2009, 25:33-43.
18. Guney M., Ozguner F., Oral B., Karahan N., Mungan T. 900MHz radiofrequency-induced histopathologic changes and oxidative stress in rat endometrium: protection by vitamins E and C. Toxicol. Ind. Health 2007, 23:411-420.
19. ICNIRP Report Guidelines for limiting exposure to time-varying electric, magnetic, and electromagnetic fields (up to 300GHz). Health Phys. 1998, 74:494-522.
20. IEEE, 2002. IEEE Recommended Practice for Measurements and Computations of Radio Frequency Electromagnetic Fields with Respect to Human Exposure to Such Fields, 100kHz-300GHz. IEEE Std C95.3-2002, The Institute of Electrical and Electronics Engineers, New York.
21. Ilhan A., Gurel A., Armutcu F., Kamisli S., Iraz M., Akyol O., Ozen S. Ginko biloba prevents mobile phone-induced oxidative stress in rat brain. Clin. Chim. Acta 2004, 340:153-162.
22. Irmak M.K., Fadillioglu E., Gulec M., Erdogan H., Yagmurca M., Akyol O. Effect of electromagnetic radiation from a cellular telephone on the oxidant and antioxidant levels in rabbits. Cell Biochem. Funct. 2002, 20:279-283.
23. Jauchem J.R. Effects of low-level radio-frequency (3kHz to 300GHz) energy on human cardiovascular, reproductive, immune, and other systems: a review of the recent literature. Int. J. Hyg. Environ. Health 2008, 211:1-29.
24. Juutilainen J. Do electromagnetic fields enhance the effects of environmental carcinogens?. Radiat. Prot. Dosimetry 2008, 132:228-231.
25. Köylü H., Mollaoglu H., Ozguner F., Naziroglu M., Delibas N. Melatonin modulates 900Mhz microwave-induced lipid peroxidation changes in rat brain. Toxicol. Ind. Health 2006, 22:211-216.
26. Marnett L.J. Oxy radicals, lipid peroxidation and DNA damage. Toxicology 2002, 181-182:219-222.
27. Meral I., Mert H., Mert N., Deger Y., Yoruk I., Yetkin A., Keskin S. Effects of 900-MHz electromagnetic fields emitted from cellular phone on brain oxidative stress and some vitamin levels of guinea pits. Brain Res. 2007, 1169:120-124.
28. Moretti M., Villarini M., Scassellati-Sforzolini G., Monarca S., Libraro M., Fatigoni C., Donato F., Leonardis C., Perego L. Biological monitoring of genotoxic hazard in workers of the rubber industry. Environ. Health Perspect. 1996, 104:543-545.
29. Moustafa Y.M., Moustafa R.M., Belacy A., Abou-El-Ela S.H., Ali F.M. Effects of acute exposure to the radiofrequency fields of cellular phones on plasma lipid peroxide and antioxidase activities in human erythrocytes. J. Pharm. Biomed. Anal. 2001, 26:605-608.
30. Perricone C., De Carolis C., Perricone R. Glutathione: a key player in autoimmunity. Autoimmun. Rev. 2009, 8:697-701.
31. Richie J.P., Skowronski L., Abraham P., Leutzinger Y. Blood glutathione concentrations in a large-scale human study. Clin. Chem. 1996, 42:64-70.
32. Singh N.P., McCoy M.T., Tice R.R., Schneider L.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 1988, 175:184-191.
33. Stopczyk D., Gnitecki W., Buczyński A., Kowalski W., Buczyńska M., Kroc A. Effect of electromagnetic field produced by mobile phones on the activity of superoxide dismutase (SOD-1) - in vitro researches. Ann. Acad. Med. Stetin. 2005, 51:125-128.
34. Sokolovic D., Djindjic B., Nikolic J., Bjelakovic G., Pavlovic D., Kocic G., Krstic D., Cvetkovic T., Pavlovic V. Melatonin reduces oxidative stress induced by chronic exposure of microwave radiation from mobile phones in rat brain. J. Radiat. Res. (Tokyo) 2008, 49:579-586.
35. Šarolić A., Modlic B. Radiation hazard aspect of shipboard radiocommunication equipment. JCOMSS 2007, 3:123-132.
36. Umegaki K., Fenech M. Cytokinesis-block micronucleus assay in WIL2-NS cells: a sensitive system to detect chromosomal damage induced by reactive oxygen species and activated human neutrophils. Mutagenesis 2000, 15:261-269.
37. Verschaeve L. Genetic effects of radiofrequency radiation (RFR). Toxicol. Appl. Pharmacol. 2005, 207:336-341.
38. Verschaeve L. Genetic damage in subjects exposed to radiofrequency radiation. Mutat. Res. 2009, 681:259-270.
39. Yadav A.S., Sharma M.K. Increased frequency of micronucleated exfoliated cells among humans exposed in vivo to mobile telephone radiations. Mutat. Res. 2008, 650:175-180.
40. Yao K., Wu W., Wang K., Ni S., Ye P., Yu Y., Ye J., Sun L. Electromagnetic noise inhibits radiofrequency radiation-induced DNA damage and reactive oxygen species increase in human lens epithelial cells. Mol. Vis. 2008, 14:964-969.
41. Yurekli A.I., Ozkan M., Kalkan T., Saybasili H., Tuncel H., Atukeren P., Gumustas K., Seker S. GSM base station electromagnetic radiation and oxidative stress in rats. Electromagn. Biol. Med. 2006, 25:177-188.
42. Zotti-Martelli L., Peccatori M., Maggini V., Ballardin M., Barale R. Individual responsiveness to induction of micronuclei in human lymphocytes after exposure in vitro to 1800-MHz microwave radiation. Mutat. Res. 2005, 582:42-52.