Partial deletion in the spike endodomain of mouse hepatitis virus decreases the cytopathic effect but maintains foreign protein expression in infected cells

Journal of Virological Methods

Volumn 172, Issue 1-2, 2011, Pages 46-53

  Yang, Jinhua ^a   Sun, Zhiping ^a   Wang, Yuyan ^a   Lv, Jun ^a   Qu, Di ^a   Ye, Rong ^a  

^a FUDAN UNIVERSITY   (China)

Author keywords

Expression; Foreign protein; MHV; Partial deletion; Spike endodomain

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; ESTERASE; VIRUS HEMAGGLUTININ; VIRUS SPIKE PROTEIN; VIRUS VECTOR;

ARTICLE; CAT; CELL STRAIN; CONTROLLED STUDY; EVOLUTIONARY ADAPTATION; EXPRESSION VECTOR; FETUS; GENE DELETION; GENE EXPRESSION; GENETIC TRANSFECTION; MURINE HEPATITIS CORONAVIRUS; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN LOCALIZATION; RAT; VIRUS CELL INTERACTION; VIRUS RECOMBINANT; VIRUS REPLICATION; VIRUS VIRULENCE;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE; CYTOPATHOGENIC EFFECT, VIRAL; DEFECTIVE VIRUSES; GENE DELETION; GENE EXPRESSION REGULATION, VIRAL; GENE ORDER; INTRACELLULAR SPACE; MICE; MOLECULAR SEQUENCE DATA; MURINE HEPATITIS VIRUS; NIH 3T3 CELLS; PHENOTYPE; PROTEIN TRANSPORT; RATS; RECOMBINANT FUSION PROTEINS; VIRAL ENVELOPE PROTEINS; VIRUS REPLICATION;

CORONAVIRUS; EUKARYOTA; MURINE HEPATITIS VIRUS; RATTUS;

EID: 79451468769     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.12.018     Document Type: Article

References (26)

1. Aiyar A., Xiang Y., Leis J. Site-directed mutagenesis using overlap extension PCR. Methods Mol. Biol. 1996, 57:177-191.
2. Baker D.G. Natural Pathogens of Laboratory Animals: Their Effects on Research 2003, ASM Press, Washington, DC.
3. Bos E.C.W., Heijnen L., Luytjes W., Spaan W.J.M. Mutational analysis of the murine coronavirus spike protein: effect on cell-to-cell fusion. Virology 1995, 214:453-463.
4. Chang K.W., Sheng Y., Gombold J.L. Coronavirus-induced membrane fusion requires the cysteine-rich domain in the spike protein. Virology 2000, 269:212-224.
5. Cushman S.W., Wardzala L.J. Potential mechanism of insulin action on glucose transport in the isolated rat adipose cell: apparent translocation of intracellular transport systems to the plasma membrane. J. Biol. Chem. 1980, 255:4758-4762.
6. Das Sarma J., Scheen E., Seo S.H., Koval M., Weiss S.R. Enhanced green fluorescent protein expression may be used to monitor murine coronavirus spread in vitro and in the mouse central nervous system. J. Neurovirol. 2002, 8:381-391.
7. de Haan C.A., Masters P.S., Shen X., Weiss S., Rottier P.J. The group-specific murine coronavirus genes are not essential, but their deletion, by reverse genetics, is attenuating in the natural host. Virology 2002, 296:177-189.
8. de Haan C.A., van Genne L., Stoop J.N., Volders H., Rottier P.J. Coronaviruses as vectors: position dependence of foreign gene expression. J. Virol. 2003, 77:11312-11323.
9. Enjuanes L., Sola I., Almazan F., Ortego J., Izeta A., Gonzalez J.M., Alonso S., Sanchez J.M., Escors D., Calvo E., Riquelme C., Sanchez C. Coronavirus derived expression systems. J. Biotechnol. 2001, 88:183-204.
10. Goebel S.J., Hsue B., Dombrowski T.F., Masters P.S. Characterization of the RNA components of a putative molecular switch in the 3' untranslated region of the murine coronavirus genome. J. Virol. 2004, 78:669-682.
11. Hurst K.R., Kuo L., Koetzner C.A., Ye R., Hsue B., Masters P.S. A major determinant for membrane protein interaction localizes to the carboxy-terminal domain of the mouse coronavirus nucleocapsid protein. J. Virol. 2005, 79:13285-13297.
12. Kuo L., Godeke G.J., Raamsman M.J., Masters P.S., Rottier P.J. Retargeting of coronavirus by substitution of the spike glycoprotein ectodomain: crossing the host cell species barrier. J. Virol. 2000, 74:1393-1406.
13. Lissenberg A., Vrolijk M.M., van Vliet A.L., Langereis M.A., de Groot-Mijnes J.D., Rottier P.J., de Groot R.J. Luxury at a cost? Recombinant mouse hepatitis viruses expressing the accessory hemagglutinin esterase protein display reduced fitness in vitro. J. Virol. 2005, 79:15054-15063.
14. Masters P.S. The molecular biology of coronaviruses. Adv. Virus Res. 2006, 66:193-292.
15. Masters P.S., Rottier P.J. Coronavirus reverse genetics by targeted RNA recombination. Curr. Top. Microbiol. Immunol. 2005, 287:133-159.
16. Mazumder R., Iyer L.M., Vasudevan S., Aravind L. Detection of novel members, structure-function analysis and evolutionary classification of the 2H phosphoesterase superfamily. Nucleic Acids Res. 2002, 30:5229-5243.
17. Ontiveros E., Kuo L., Masters P.S., Perlman S. Inactivation of expression of gene 4 of mouse hepatitis virus strain JHM does not affect virulence in the murine CNS. Virology 2001, 289:230-238.
18. Perlman S., Butler N. Neurotropic coronavirus infection. Neurotropic Viral Infections 2008, 50-74. Cambridge University Press, Cambridge, UK. C.S. Reiss (Ed.).
19. Sawicki S.G., Sawicki D.L. A new model for coronavirus transcription. Adv. Exp. Med. Biol. 1998, 440:215-219.
20. The Coronaviridae 1995, Plenum Press, New York, NY. S.G. Siddell (Ed.).
21. Snijder E.J., Bredenbeek P.J., Dobbe J.C., Thiel V., Ziebuhr J., Poon L.L., Guan Y., Rozanov M., Spaan W.J., Gorbalenya A.E. Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J. Mol. Biol. 2003, 331:991-1004.
22. Spaan W., Cananagh D., de Groot R., Enjuanes L., Snijder E., Walker P. Order Nidovirales. Virus Taxonomy: Classification and Nomenclature of Viruses. Eighth Report of the International Committee on Taxonomy of Viruses 2005, 937-945. Elsevier Academic Press, San Diego, CA. C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball (Eds.).
23. Stohlman S.A., Bergmann C.C., Perlman S. Mouse hepatitis virus. Persistent Viral Infection 1998, 537-557. John Wiley and Sons Ltd., New York, NY. R. Ahmed, I. Chen (Eds.).
24. Thorp E.B., Boscarino J.A., Logan H.L., Goletz J.T., Gallagher T.M. Palmitoylations on murine coronavirus spike proteins are essential for virion assembly and infectivity. J. Virol. 2006, 80:1280-1289.
25. Weiss S.R., Navas-Martin S. Coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus. Microbiol. Mol. Biol. Rev. 2005, 69:635-664.
26. Ye R., Montalto-Morrison C., Masters P.S. Genetic analysis of determinants for spike glycoprotein assembly into murine coronavirus virions: distinct roles for charge-rich and cysteine-rich regions of the endodomain. J. Virol. 2004, 78:9904-9917.