Activation of p53 by scaffold-stabilised expression of Mdm2-binding peptides: Visualisation of reporter gene induction at the single-cell level

British Journal of Cancer

Volumn 91, Issue 8, 2004, Pages 1488-1494

  Karlsson, G B ^a,c   Jensen, A ^a,d   Stevenson, L F ^b   Woods, Y L ^b   Lane, D P ^b   Sorensen M S ^a,e  

^a Pharmexa AS   (Denmark)

^b UNIVERSITY OF DUNDEE   (United Kingdom)

^c KAROLINSKA INSTITUTE   (Sweden)

^d Symphogen AS   (Denmark)

^e Zealand Pharma AS   (Denmark)

Author keywords

p53 activity; Peptides; Retroviral vector; Scaffold protein

Indexed keywords

BINDING PROTEIN; CELL PROTEIN; PROTEIN MDM2; PROTEIN P53; RETROVIRUS VECTOR; BETA GALACTOSIDASE; CARRIER PROTEIN; GROWTH FACTOR RECEPTOR; MDM2 PROTEIN, HUMAN; NERVE GROWTH FACTOR RECEPTOR; NERVE PROTEIN; NGFR PROTEIN, HUMAN; NUCLEAR PROTEIN; ONCOPROTEIN; PEPTIDE FRAGMENT; PROTEIN P14ARF;

ARTICLE; CYTOLOGY; CYTOLYSIS; FLOW CYTOMETRY; GENE CONSTRUCT; GENE INDUCTION; GENE TARGETING; GENETIC CODE; GENETIC TRANSFECTION; IMMUNOPRECIPITATION; MAMMAL CELL; NONHUMAN; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; REPORTER GENE; SIGNAL TRANSDUCTION; BONE TUMOR; CELL CULTURE; GENE VECTOR; GENETICS; HUMAN; METABOLISM; OSTEOSARCOMA; PATHOLOGY; PROTEIN BINDING; RETROVIRUS;

BETA-GALACTOSIDASE; BONE NEOPLASMS; CARRIER PROTEINS; GENETIC VECTORS; HUMANS; NERVE TISSUE PROTEINS; NUCLEAR PROTEINS; OSTEOSARCOMA; PEPTIDE FRAGMENTS; PROTEIN BINDING; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-MDM2; RECEPTORS, GROWTH FACTOR; RECEPTORS, NERVE GROWTH FACTOR; RETROVIRIDAE; TRANSFECTION; TUMOR CELLS, CULTURED; TUMOR SUPPRESSOR PROTEIN P14ARF; TUMOR SUPPRESSOR PROTEIN P53;

EID: 7944237727     PISSN: 00070920     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjc.6602143     Document Type: Article

References (25)

1. Abedi MR, Caponigro G, Kamb A (1998) Green fluorescent protein as a scaffold for intracellular presentation of peptides. Nucleic Acids Res 26: 623-630
2. Bartek J, Bartkova J, Lukas J, Staskova Z, Vojtesek B, Lane DP (1993) Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies. J Pathol 169: 27-34
3. Bottger A, Bottger V, Sparks A, Liu WL, Howard SF, Lane DP (1997) Design of a synthetic Mdm2-binding mini protein that activates the p53 response in vivo. Curr Biol 7: 860-869
4. Bottger V, Bottger A, Howard SF, Picksley SM, Chene P, Garcia-Echeverria C, Hochkeppel HK, Lane DP (1996) Identification of novel mdm2 binding peptides by phage display. Oncogene 13: 2141-2147
5. Chen J, Marechal V, Levine AJ (1993) Mapping of the p53 and mdm-2 interaction domains. Mol Cell Biol 13: 4107-4114
6. Duch M, Tolstrup A, Dalum I, Jespersen T, Mouritsen S, Pedersen FS (1999) Functional testing of a bicistronic retroviral vector for intracellular peptide production. Biotechniques 26: 1032-1034, 1036
7. Gururaja TL, Narasimhamurthy S, Payan DG, Anderson DC (2000) A novel artificial loop scaffold for the noncovalent constraint of peptides. Chem Biol 7: 515-527
8. Hitoshi Y, Gururaja T, Pearsall DM, Lang W, Sharma P, Huang B, Catalano SM, McLaughlin J, Pali E, Peelle B, Vialard J, Janicot M, Wouters W, Luyten W, Bennett MK, Anderson DC, Payan DG, Lorens JB, Bogenberger J, Demo S (2003) Cellular localization and antiproliferative effect of peptides discovered from a functional screen of a retrovirally delivered random peptide library. Chem Biol 10: 975-987
9. Honda R, Tanaka H, Yasuda H (1997) Oncoprotein MDM2 is a ubiquitin ligase E3 for tumor suppressor p53. FEBS Lett 420: 25-27
10. Hupp TR, Sparks A, Lane DP (1995) Small peptides activate the latent sequence-specific DNA binding function of p53. Cell 83: 237-245
11. Ladner RC (1995) Constrained peptides as binding entities. Trends Biotechnol 13: 426-430
12. Mavilio F, Ferrari G, Rossini S, Nobili N, Bonini C, Casorati G, Traversari C, Bordignon C (1994) Peripheral blood lymphocytes as target cells of retroviral vector-mediated gene transfer. Blood 83: 1988-1997
13. McPhalen CA, James MN (1987) Crystal and molecular structure of the serine proteinase inhibitor CI-2 from barley seeds. Biochemistry 26: 261-269
14. Nolan GP, Fiering S, Nicolas JF, Herzenberg LA (1988) Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ. Proc Natl Acad Sci USA 85: 2603-2607
15. Nygren PA, Uhlen M (1997) Scaffolds for engineering novel binding sites in proteins. Curr Opin Struct Biol 7: 463-469
16. Osmark P, Sorensen P, Poulsen FM (1993) Context dependence of protein secondary structure formation: the three-dimensional structure and stability of a hybrid between chymotrypsin inhibitor 2 and helix E from subtilisin Carlsberg. Biochemistry 32: 11007-11014
17. Peelle B, Lorens J, Li W, Bogenberger J, Payan DG, Anderson DC (2001) Intracellular protein scaffold-mediated display of random peptide libraries for phenotypic screens in mammalian cells. Chem Biol 8: 521-534
18. Souroujon MC, Mochly-Rosen D (1998) Peptide modulators of protein-protein interactions in intracellular signaling. Nat Biotechnol 16: 919-924
19. Stephen CW, Helminen P, Lane DP (1995) Characterisation of epitopes on human p53 using phage-displayed peptide libraries: insights into antibody-peptide interactions. J Mol Biol 248: 58-78
20. Tolstrup AB, Duch M, Dalum I, Pedersen FS, Mouritsen S (2001) Functional screening of a retroviral peptide library for MHC class I presentation. Gene 263: 77-84
21. Vassilev LT, Vu BT, Graves B, Carvajal D, Podlaski F, Filipovic Z, Kong N, Kammlott U, Lukacs C, Klein C, Fotouhi N, Liu EA (2004) In vivo activation of the p53 pathway by small-molecule antagonists of MDM2. Science 303: 844-848
22. Weber JD, Kuo ML, Bothner B, DiGiammarino EL, Kriwacki RW, Roussel MF, Sherr CJ (2000) Cooperative signals governing ARF-mdm2 interaction and nucleolar localization of the complex. Mol Cell Biol 20: 2517-2528
23. Xu X, Leo C, Jang Y, Chan E, Padilla D, Huang BC, Lin T, Gururaja T, Hitoshi Y, Lorens JB, Anderson DC, Sikic B, Luo Y, Payan DG, Nolan GP (2001) Dominant effector genetics in mammalian cells. Nat Genet 27: 23-29
24. Zheleva DI, Lane DP, Fischer PM (2003) The p53-Mdm2 pathway: targets for the development of new anticancer therapeutics. Mini Rev Med Chem 3: 257-270
25. Zwick MB, Shen J, Scott JK (1998) Phage-displayed peptide libraries. Curr Opin Biotechnol 9: 427-436