Detection of single molecules illuminated by a light-emitting diode

Sensors

Volumn 11, Issue 1, 2011, Pages 905-916

  Gerhardt, Ilja ^a,b   Mai, Lijian ^a   Lamas Linares, Antía ^a   Kurtsiefer, Christian ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Fluorescence microscopy; LED; Light emitting diode; Signal to noise ratio; Single molecules; Single photon detection

Indexed keywords

EID: 79251648587     PISSN: 14248220     EISSN: None     Source Type: Journal    
DOI: 10.3390/s110100905     Document Type: Article

References (18)

1. Hirschfeld, T. Optical microscopic observation of single small molecules. Appl. Opt. 1976, 15, 2965-2966.
2. Moerner, W.E.; Kador, L. Optical detection and spectroscopy of single molecules in a solid. Phys. Rev. Lett. 1989, 62, 2535-2538.
3. Orrit, M.; Bernard, J. Single pentacene molecules deteced byfluorescence excitation in a p-terphenyl crystal. Phys. Rev. Lett. 1990, 65, 2716-2719.
4. Betzig, E.; Chichester, R.J. Single molecules observed by near-field scanning optical microscopy. Science 1993, 262, 1422-1425.
5. Hell, S.W. Toward fluorescence nanoscopy. Nature Biotechn. 2003, 21, 1347-1355.
6. Unger, M.; Kartalov, E.; Chiu, C.S.; Lester, H.; Quake, S. Single-molecule fluorescence observed with mercury lamp illumination. BioTechniques 1999, 27, 1008-1014.
7. Kuo, J.S.; Kuyper, C.L.; Allen, P.B.; Fiorini, G.S.; Chiu, D.T. High-power blue/UV light-emitting diodes as excitation sources for sensitive detection. Electrophoresis 2004, 25, 3796-3804.
8. Hattori, A.; Habuchi, S.; Vacha, M. Single-molecule imaging with an inexpensive UV-LED light source. Chem. Lett. 2009, 38, 234-235.
9. Basché, T.; Moerner, W.E.; Orrit, M.; Talon, H. Photon antibunching in the fluorescence of a single dye molecule trapped in a solid. Phys. Rev. Lett. 1992, 69, 1516-1519.
10. Pfab, R.; Zimmermann, J.; Hettich, C.; Gerhardt, I.; Renn, A.; Sandoghdar, V. Aligned terrylene molecules in a spin-coated ultrathin crystalline film of p-terphenyl. Chem. Phys. Lett. 2004, 387, 490-495.
11. Kummer, S.; Kulzer, F.; Kettner, R.; Basché, T.; Tietz, C.; Glowatz, C.; Kryschi, C. Absorption, excitation, and emission spectroscopy of terrylene in p-terphenyl: Bulk measurements and single molecule studies. J. Chem. Phys. 1997, 107, 7673-7684.
12. Kulzer, F.; Koberling, F.; Christ, T.; Mews, A.; Basché, T. Terrylene in p-terphenyl: Single-molecule experiments at room temperature. Chem. Phys. 1999, 247, 23-34.
13. Thompson, R.E.; Larson, D.R.; Webb, W.W. Precise nanometer localization analysis for individual fluorescent probes. Biophys. J. 2002, 82, 2775-2783.
14. Peter, M.; Laubsch, A.; Bergbauer, W.; Meyer, T.; Sabathil, M.; Baur, J.; Hahn, B. New developments in green LEDs. Phys. Stat. Sol. A 2009, 206, 1125-1129.
15. Kukura, P.; Celebrano, M.; Renn, A.; Sandoghdar, V. Imaging a single quantum dot when it is dark. Nano Lett. 2008, 9, 926929.
16. Moerner, W.E.; Plakhotnik, T.; Irngatinger, T.; Croci, M.; Palm, V.; Wild, U.P. Optical probing of single molecules of terrylene in a Shpol'skii matrix: A two-state single-molecule switch. J. Phys. Chem. 1994, 98, 7382-7389.
17. Bushberg, J.T.; Seibert, J.A.; Jr, E.M.L.; Boone, J.M. The Essential Physics of Medical Imaging, 2nd ed.; Lippincott Williams & Wilkins: Philadelphia, PA, USA, 2001.
18. Loudon, R. The Quantum Theory of Light; Oxford University Press: New York, NY, USA, 2000.