Metabolite extraction from adherently growing mammalian cells for metabolomics studies: Optimization of harvesting and extraction protocols

Analytical and Bioanalytical Chemistry

Volumn 399, Issue 3, 2011, Pages 1127-1139

  Dettmer, Katja ^a   Nürnberger, Nadine ^a   Kaspar, Hannelore ^a   Gruber, Michael A ^b   Almstetter, Martin F ^a   Oefner, Peter J ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b UNIVERSITY HOSPITAL REGENSBURG   (Germany)

Author keywords

Adherent cells; Amino acids; Detachment; Extraction; GC MS; Metabolomics

Indexed keywords

ACID BASE; ADHERENT CELLS; BUFFER SOLUTIONS; CELL PELLETS; CERAMIC BEADS; CHLOROFORMATES; DERIVATIZATIONS; DETACHMENT; ENERGY METABOLISM; EXTRACTION EFFICIENCIES; EXTRACTION PROTOCOLS; EXTRACTION SOLVENTS; EXTRACTION YIELD; FREEZE/THAW CYCLES; GC-MS; MAMMALIAN CELLS; METABOLIC FINGERPRINTS; METABOLITE EXTRACTION; METABOLOMICS; REPRODUCIBILITIES; ULTRA-SONICATION;

ACETONE; AMINO ACIDS; BIOMOLECULES; MAMMALS; METABOLISM; METABOLITES; METHANOL; POSITIVE IONS;

SOLVENT EXTRACTION;

2 PROPANOL; ACETONE; AMINO ACID; CHLOROFORM; EDETIC ACID; METHANOL; SOLVENT; TRYPSIN; WATER;

ARTICLE; CELL ADHESION; CELL PROLIFERATION; CHEMISTRY; DRUG EFFECT; ENERGY METABOLISM; HUMAN; MASS FRAGMENTOGRAPHY; METABOLISM; TUMOR CELL CULTURE;

2-PROPANOL; ACETONE; AMINO ACIDS; CELL ADHESION; CELL PROLIFERATION; CHLOROFORM; EDETIC ACID; ENERGY METABOLISM; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; METHANOL; SOLVENTS; TRYPSIN; TUMOR CELLS, CULTURED; WATER;

MAMMALIA;

EID: 79251624872     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-010-4425-x     Document Type: Article

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