Identification of chitinases Is-chiA and Is-chiB from Isoptericola jiangsuensis CLG and their characterization

Applied Microbiology and Biotechnology

Volumn 89, Issue 3, 2011, Pages 705-713

  Wu, Ying ^a,b   Liu, Fang ^a   Liu, Yi Chen ^a   Zhang, Zhen Hua ^a   Zhou, Tian Tian ^a   Liu, Xin ^a   Shen, Qi Rong ^a   Shen, Biao ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

^b Administration Committee of Wuxi Bio pharmaceutical R and D Outsourcing Service Park   (China)

Author keywords

Biological characteristics; Chitinase; Cloning and expression; Is chiA and Is chiB

Indexed keywords

ARTHROBACTER SP; BIOLOGICAL CHARACTERISTIC; CHITINASES; CLONING AND EXPRESSION; DNA FRAGMENT; DNA SEQUENCING ANALYSIS; ENDOCHITINASE; EXPRESSION VECTORS; GENOMIC DNA; IS-CHIA AND IS-CHIB; NI-NITRILOTRIACETIC ACID; OPEN READING FRAME; PCR PRIMERS; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; CLONING; DNA; DNA SEQUENCES; ELECTROPHORESIS; ESCHERICHIA COLI; GENE ENCODING; GENE EXPRESSION; SODIUM; SODIUM SULFATE;

POLYMERASE CHAIN REACTION;

AMINO ACID; CHITINASE; CHITOBIOSIDASE; DNA FRAGMENT; ENDOCHITINASE; GENOMIC DNA; GLYCOSIDASE; IS CHIA PROTEIN; IS CHIB PROTEIN; ISOPROPYL THIOGALACTOSIDE; UNCLASSIFIED DRUG;

BACTERIUM; CHROMATOGRAPHY; CLONE; ELECTROKINESIS; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC ANALYSIS; GENOMICS; POLYMERASE CHAIN REACTION;

ARTHROBACTER; ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATION; CHROMATOGRAPHY; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE EXPRESSION; GENE SEQUENCE; IS CHIA GENE; IS CHIB GENE; ISOPTERICOLA JIANGSUENSIS; MOLECULAR CLONING; NINITRILOTRIACETIC ACID AFFINITY CHROMATOGRAPHY; NONHUMAN; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

ACTINOMYCETALES; ARTHROBACTER; CHITINASE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA PRIMERS; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; HEXOSAMINIDASES; MOLECULAR WEIGHT; OPEN READING FRAMES; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

ARTHROBACTER SP.; ESCHERICHIA COLI; ISOPTERICOLA;

EID: 79251598508     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2917-4     Document Type: Article

References (31)

1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1999) Short protocals in molecular Biology, 4th edn. Wiley, New York
2. MM Bradford 1979 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 248 254 10.1016/0003-2697(76)90527-3
3. E Cabib 1987 The synthesis and degradation of chitin Adv Enzymol Relat Areas Mol Biol 59 59 101 1:CAS:528:DyaL2sXhtFGhuro%3D
4. MT Cottrell JA Moore DL Kirchman 1999 Chitinase from uncultured marine microorganisms Appl Environ Microbiol 65 2553 2557 1:CAS:528:DyaK1MXjs12gtr0%3D
5. AP Gleave RK Taylor BA Morris DR Greenwood 1995 Cloning and sequencing of a gene encoding the 69-kDa extracellular chitinase of Janthinobacterium lividum FEMS Microbiol Lett 131 279 288 1:CAS:528:DyaK2MXosFGisrg%3D 10.1111/j.1574-6968.1995.tb07788.x
6. B Henrissat 1991 A classification of glycosyl hydrolases based on amino acid sequence similaritites J Biochem 280 309 316 1:CAS:528:DyaK3MXmslCmu78%3D
7. F Hoster JE Schmitz R Daniel 2005 Enrichment of chitinolytic microorganisms: isolation and characterization of a chitinase exhibiting antifungal activity against phytopathogenic fungi from a novel Streptomyces strain Appl Microbiol Biotechnol 66 434 442 1:CAS:528:DC%2BD2cXhtVKrur%2FE 10.1007/s00253-004-1664-9
8. NO Keyhani S Roseman 1996 The chitin catabolic cascade in the marine bacterium Vibrio furnissii: molecular cloning, isolation, and characterization of a periplasmic chitodextrinase J Biol Chem 271 33414 33424 1:CAS:528:DyaK2sXitFeqsQ%3D%3D 10.1074/jbc.271.52.33414
9. UK Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680 685 1:CAS:528:DC%2BD3MXlsFags7s%3D 10.1038/227680a0
10. H Li H Plattner KL Schimz M Kieß H Diekmann J Meens 2000 Cloning, sequencing and heterologous expression of a new chitinase gene, chi92, from Streptomyces olivaceoviridis ATCC 11238 Biotechnol Lett 22 1203 1209 1:CAS:528:DC%2BD3cXmsFGrsrw%3D 10.1023/A:1005694532044
11. T Lonhienne K Mavromatis CE Vorgias L Buchon C Gerday V Bouriotis 2001 Cloning, sequences and characterization of two chitinase genes from the antarctic Arthrobacter sp. strain TAD20: isolation and partial characterization of the enzymes J Bacteriol 183 1773 1779 1:CAS:528:DC%2BD3MXhtlOqs74%3D 10.1128/JB.183.5.1773-1779.2001
12. SK Park CW Kim H Kim JS Jung GE Harman 2007 Cloning and high-level production of a chitinase from Chromobacterium sp. and the role of conserved or nonconserved residues on its catalytic activity Appl Microbiol Biotechnol 74 791 804 1:CAS:528:DC%2BD2sXitVKjsL0%3D 10.1007/s00253-006-0614-0
13. RSV Patil DMV Ghormade 2000 Chitinolytic enzymes: an exploration Enzyme Microb Technol 26 473 83 1:CAS:528:DC%2BD3cXisFGrtr8%3D 10.1016/S0141-0229(00) 00134-4
14. A Perrakis I Tews Z Dauter AB Oppenheim I Chet KS Wilson CE Vorgias 1994 Crystal structure of a bacterial chitinase at 2.3 Å resolution Structure 2 1169 1180 1:CAS:528:DyaK2MXjs1yit78%3D 10.1016/S0969-2126(94)00119-7
15. N Ramaiah RT Hill J Chun J Ravel MH Matte WL Straube RR Colwell 2000 Use of a chiA probe for detection of chitinase genes in bacteria from the Chesapeake Bay FEMS Microbiol Ecol 34 63 71 1:CAS:528:DC%2BD3cXosVyrtL0%3D
16. G Reguera SB Leschine 2001 Chitin degradation by cellulolytic anaerobes and facultative aerobes from soils and sediments FEMS Microbiol Lett 204 367 374 1:CAS:528:DC%2BD3MXos1Kiuro%3D 10.1111/j.1574-6968.2001.tb10912.x
17. G Reguera SB Leschine 2003 Biochemical and genetic characterization of ChiA, the major enzyme component for the solubilization of Cellulomonas uda Arch Microbiol 180 434 443 1:CAS:528:DC%2BD3sXpsVOitrw%3D 10.1007/s00203-003-0611-y
18. K Sakai A Yakota H Kurokawa M Wakayama M Moriguchi 1998 Purification and characterization of three thermostable endochitinases of Bacillus noble strain MH-1 isolated from chitin containing compost Appl Environ Microbiol 64 9 3340 3397
19. AL Svitil DL Kirchman 1998 A chitin-binding domain in a marine bacterial chitinase and other microbial chitinases: implications for the ecology and evolution of 1, 4-β-glycanase Microbiology 144 1299 1308 1:CAS:528:DyaK1cXjsVagtrk%3D 10.1099/00221287-144-5-1299
20. T Tanaka S Fujiwara S Nishikori T Fukui M Takagi T Imanaka 1999 A unique chitinase with dual active sites and triple substrate binding sited from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 Appl Environ Microbiol 65 5338 5344 1:CAS:528:DyaK1MXnvVGjt7k%3D
21. S Techkarnjanaruk S Pongpattanakhitshote AE Goodman 1997 Use of a promoterless lacZ gene insertion to investigate chitinase gene expression in the marine bacterium Pseudoalteromonas sp. strain S9 Appl Environ Microbiol 63 2989 2996 1:CAS:528:DyaK2sXlt1Wjur0%3D
22. S Thamthiankul S Suan-Ngay S Tantimavanich 2001 Chitinase from Bacillus thuringiensis subsp. pakistani Appl Microbiol Biotechnol 56 395 401 1:CAS:528:DC%2BD3MXmsFKhurk%3D 10.1007/s002530100630
23. A Tronsmo GE Harman 1993 Detection and quantification of N-acetyl-β-D-glucosaminidase, chitobiosidase, and endochitinase in solutions and on gels Anal Biochem 208 74 79 1:CAS:528:DyaK3sXhs1ektro%3D 10.1006/abio.1993.1010
24. I Tsigo V Bouriotis 1995 Purification and characterization of chitin deacetylase from Colletotrichum lindemuthianum J Biol Chem 270 26280 91
25. H Tsujibo H Orikoshi N Baba M Miyahara K Miyamoto M Yasuda Y Inamori 2002 Identification and characterization of the gene cluster involved in chitin degradation in a marine bacterium, Alteromonas sp. strain O-7 Appl Environ Microbiol 68 263 270 1:CAS:528:DC%2BD38Xjt1Wmsg%3D%3D 10.1128/AEM.68.1.263-270. 2002
26. M Ueda M Arai 1992 Purification and some properties of chitinases from Aeromonas sp. no. 10S-24 Biosci Biotechnol Biochem 56 3 460 464 1:CAS:528:DyaK38XitlSmt7Y%3D 10.1271/bbb.56.460
27. S Wang J He Z Cui S Li 2007 Self-formed adaptor PCR: a simple and efficient method for chromosome walking Appl Environ Microbiol 73 5048 5051 1:CAS:528:DC%2BD2sXptVyhu78%3D 10.1128/AEM.02973-06
28. SL Wang WT Chang 1997 Purification and characterization of two bifunctional chitinase/lysozymes extracellularly produced by Pseudomonas aeruginosa K-187 in a shrimp and crabshell powder medium Appl Environ Microbiol 63 2 380 386 1:CAS:528:DyaK2sXhtVKkt7Y%3D
29. T Watanabe K Suzuki W Oyanagi K Ohnishi H Tanaka 1990 Gene cloning of chitinase A1 from Bacillus circulans WL-12 revealed its evolutionary relationship to Serratia chitinase and to the type III homology units of fibronectin J Biol Chem 265 15659 15665 1:CAS:528:DyaK3MXjs1Ogug%3D%3D
30. T Watanabe Y Ito T Yamada M Hashimoto S Sekine T Tanaka 1994 The roles of the C-terminal domain and type III domains of chitinase A1 from Bacillus circulans WL-12 in chitin degradation J Bacteriol 176 4465 4472 1:CAS:528:DyaK2cXlt12ltrc%3D
31. Y Wu WJ Li W Tian LP Zhang L Xu QR Shen B Shen 2010 Isoptericola jiangsuensis sp. nov., a chitin-degrading bacterium Int J Syst Evol Microbiol 60 904 908 1:CAS:528:DC%2BC3cXmvFWns74%3D 10.1099/ijs.0.012864-0