Promoter methylation and expression changes of CDH1 and P16 genes in invasive breast cancer and adjacent normal breast tissue

Neoplasma

Volumn 57, Issue 5, 2010, Pages 465-472

  Celebiler Cavusoglu, A ^a   Sevinc, A I ^a   Saydam, S ^b   Canda, T ^b   Baskan, Z ^b   Kilic, Y ^b   Sakizli, M ^b  

^a DOKUZ EYLUL UNIVERSITY   (Turkey)

^b Department of General Surgery ^*

Author keywords

Breast cancer; CDH1; Methylation; P16

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR 2; ESTROGEN; ESTROGEN RECEPTOR; MESSENGER RNA; PROTEIN P16; UVOMORULIN; CADHERIN; CDH1 PROTEIN, HUMAN;

ADULT; AGE; ARTICLE; BREAST; BREAST CANCER; CANCER INVASION; CANCER PATIENT; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CORRELATIONAL STUDY; DISEASE SEVERITY; DNA METHYLATION; FEMALE; GENE EXPRESSION; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; LYMPH NODE METASTASIS; MAJOR CLINICAL STUDY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROMOTER REGION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; AGED; BREAST TUMOR; GENETICS; METABOLISM; MIDDLE AGED; TUMOR SUPPRESSOR GENE;

ADULT; AGED; BREAST; BREAST NEOPLASMS; CADHERINS; DNA METHYLATION; FEMALE; GENES, P16; HUMANS; MIDDLE AGED; PROMOTER REGIONS, GENETIC;

EID: 79251594654     PISSN: 00282685     EISSN: None     Source Type: Journal    
DOI: 10.4149/neo_2010_05_465     Document Type: Article

References (34)

1. Egger G, Liang G, Aparicio A, Jones PA Epigenetics in human disease and prospects for epigenetic therapy. Nature 2004; 429: 457-63 doi:10.1038/nature02625
2. Widschwendter M, Jones PA DNA methylation and breast carcinogenesis. Oncogene 2002; 21: 5462-82 doi:10.1038/ sj.onc.1205606
3. Mehrotra J, Vali M, McVeigh M, Kominsky SL, Fackler MJ, et al. Very high frequency of hypermethylated genes in breast cancer metastasis to the bone, brain, and lung. Clin Cancer Res 2004; 10: 3104-9. doi:10.1158/1078-0432.CCR-03-0118
4. Widschwendter M, Siegmund KD, Muller HM, Fiegl H, Marth C, et al. Association of breast cancer DNA methylation profiles with hormone receptor status and response to tamoxifen. Cancer Res 200; 64: 3807-13.
5. Heard E, Clerc P, Avner P. X-chromosome inactivation in mammals. Annu Rev Genet. 1997; 31: 571-610. doi:10.1146/ annurev.genet.31.1.571
6. Overduin M, Harvey TS, Bagby S, Tong KI, Yau P, et al. Solution structure of the epithelial cadherin domain responsible for selective cell adhesion. Science 1995; 267: 386-9. doi:10.1126/ science.7824937
7. Graff JR, Gabrielson E, Fujii H, Baylin SB, Herman JG. Methylation patterns of the E-cadherin 5' CpG island are unstable and reflect the dynamic, heterogeneous loss of E-cadherin expression during metastatic progression. J. Biol. Chem. 2000; 275: 2727-32 doi:10.1074/jbc.275.4.2727
8. WHO. World Health Organization: International of diseases for oncology 2nd edition. World Health Organization, Geneva; 1990.
9. Simpson JF, Gray R, Dressler LG, Cobau CD, Falkson CI, et al. Prognostic value of histologic grade and proliferative activity in axillary node-positive breast cancer: results from the Eastern Cooperative Oncology Group Companion Study, EST 4189. J Clin Oncol. 2000;18: 2059-69.
10. Singletary SE, Connolly JL. Breast Cancer Staging: Working With the Sixth Edition of the AJCC Cancer Staging Manual CA Cancer J Clin. 2006; 56: 37-47 doi:10.3322/canjclin.56.1.37
11. Andersen CL, Jensen JL, Orntoft TF. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004; 64: 5245-50. doi:10.1158/0008-5472.CAN-04-0496
12. Herman JG, Graff JR, Myöhänen S, Nelkin BD, Baylin SB Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA 1996; 93: 9821-6. doi:10.1073/pnas.93.18.9821
13. Huggett J, Dheda K, Bustin S, Zumla A. Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 2005; 6: 279-284. doi:10.1038/sj.gene.6364190
14. Lyng MB, Laenkholm AV, Pallisgaard N, Ditzel HJ. Identification of genes for normalization of real-time RT-PCR data in breast carcinomas BMC Cancer 2008 (22); 8: 20. doi:10.1186/1471-2407-8-20
15. Li S, Rong M, Iacopetta B. DNA hypermethylation in breast cancer and its association with clinicopathological features. Cancer Lett 2006; 237: 272-80. doi:10.1016/j.canlet.2005.06.011
16. Vallian S, Sedaghat M, Nassiri I, Frazmand A. Methylation status of p16 INK4A tumor suppressor gene in Iranian patients with sporadic breast cancer. J Cancer Res Clin Oncol. 2009; 135(8): 991-6 doi:10.1007/s00432-008-0534-8
17. Tao MH, Shields PG, Nie J, Millen A, Ambrosone CB, et al. DNA hypermethylation and clinicopathological features in breast cancer: the Western New York Exposures and Breast Cancer (WEB) Study. Breast Cancer Res Treat. 2009 Apr; 114: 559-68 doi:10.1007/s10549-008-0028-z
18. Quach N, Goodman MF, Shibata D. In vitro mutation artifacts after formalin fixation and error prone translation synthesis during PCR. BMC Clinical Pathology 2004 Feb 12; 4: 1 doi:10.1186/1472-6890-4-1
19. Gonzalez-Zulueta M, Bender CM, Yang AS, Nguyen T, Beart RW, et al. Methylation of the 5' CpG island of the p16/CDKN2 tumor suppressor gene in normal and transformed human tissues correlates with gene silencing. Cancer Res. 1995 Oct 15; 55: 4531-5
20. Shim YH, Kang GH, Ro JY. Correlation of p16 hypermethylation with p16 protein loss in sporadic gastric carcinomas. Lab Invest, 2000; 80: 689-95
21. Salvesen HB, Das S, Akslen LA. Loss of nuclear p16 protein expression is not associated with promoter methylation but defines a subgroup of aggressive endometrial carcinomas with poor prognosis. Clin Cancer Res 2000; 6: 153-9
22. Liu Z, Wang LE, Wang L, Lu KH, Mills GB, et al. Methylation and messenger RNA expression of p15INK4b but not p16INK4a are independent risk factors for ovarian cancer. Clin Cancer Res 2005; 11: 4968-76 doi:10.1158/1078-0432.CCR-04-2293
23. Hui R, Macmillan RD, Kenny FS, Musgrove EA, Blamey RW, et al. INK4a gene expression and methylation in primary breast cancer: overexpression of p16INK4a messenger RNA is a marker of poor prognosis. Clin Cancer Res. 2000; 6: 2777-87
24. Milde-Langosch K, Bamberger AM, Rieck G, Kelp B, Löning T. Overexpression of the p16 Cell Cycle Inhibitor in Breast Cancer is Associated with a More Malignant Phenotype Breast Cancer Res Treat. 2001; 67: 61-70
25. Hazan RB, Qiao R, Keren R, Badano I, Suyama K. Cadherin switch in tumor progression. Ann N Y Acad Sci 2004; 1014: 155-63. doi:10.1196/annals.1294.016
26. Yoshida R, Kimura N, Harada Y, Ohuchi N. The loss of E-cadherin,-and ß-catenin expression is associated with metastasis and poor prognosis in invasive breast cancer. Int J Oncol 2001; 18: 513-20.
27. Lehr HA, Folpe A, Yaziji H, Kommoss F, Gown AM Cytokeratin 8 immunostaining pattern and E-cadherin expression distinguish lobular from ductal breast carcinoma. Am J Clin Pathol. 2000; 114: 190-6 doi:10.1309/CPUX-KWEH-7B26-YE19
28. Wahed A, Connelly J, Reese T E-cadherin expression in pleomorphic lobular carcinoma: an aid to differentiation from ductal carcinoma. Ann Diagn Pathol. 2002; 6: 349-51 doi:10.1053/adpa.2002.36660
29. Korkola JE, DeVries S, Fridlyand J, Hwang ES, Estep AL, et al. Differentiation of lobular versus ductal breast carcinomas by expression microarray analysis. Cancer Res 2003; 63: 7167-75
30. Zhao H, Langerod A, Ji Y, Nowels KW, Nesland JM, et al. Different gene expression patterns in invasive lobular and ductal carcinomas of the breast. Mol Biol Cell 2004; 15: 2523-36 doi:10.1091/mbc.E03-11-0786
31. Acs G, Lawton TJ, Rebbeck TR, LiVolsi VA, Zhang PJ. Differential expression of E-cadherin in lobular and ductal neoplasms of the breast and its biologic and diagnostic implications. Am J Clin Pathol 2001; 115: 85-98. doi:10.1309/FDHX-L92RBATQ-2GE0
32. Yan PS, Venkataramu C, Ibrahim A, Liu JC, Shen RZ, et al Mapping geographic zones of cancer risk with epigenetic biomarkers in normal breast tissue. Clin Cancer Res. 2006 Nov 15; 12: 6626-36. doi:10.1158/1078-0432.CCR-06-0467
33. Eads CA, Danenberg KD, Kawakami K, Saltz LB, Blake C, et al. MethyLight: a high-throughput assay to measure DNA methylation. Nucleic Acids Res, 2000 Apr 15; 28(8): E32. doi:10.1093/nar/28.8.e32
34. Tripathi A, King C, de la Morenas A, Perry VK, Burke B, et al Gene expression abnormalities in histologically normal breast epithelium of breast cancer patients. Int J Cancer. 2008 Apr 1;122: 1557-66 doi:10.1002/ijc.23267