An extracellullar nuclease from Bacillus firmus VKPACU-1: Specificity and mode of action

Nucleosides, Nucleotides and Nucleic Acids

Volumn 30, Issue 1, 2011, Pages 20-30

  Kumar, Periasamy Ashok ^a   Kannan, Vijayaraghavan ^a  

^a UNIVERSITY OF MADRAS   (India)

Author keywords

Bacillus firmus; endo mode of action; Extracellular nuclease; multifunctional enzyme

Indexed keywords

ADENOSINE PHOSPHATE; DEOXYADENOSINE PHOSPHATE; DEOXYGUANOSINE PHOSPHATE; DEOXYURIDINE PHOSPHATE; DNA; GUANOSINE PHOSPHATE; HYDROXYL GROUP; NUCLEASE; OLIGONUCLEOTIDE; PHOSPHATE; POLYADENYLIC ACID; POLYCYTIDYLIC ACID; POLYGUANYLIC ACID; POLYURIDYLIC ACID; RNA; URIDINE PHOSPHATE;

ARTICLE; BACILLUS; BACILLUS FIRMUS; CONTROLLED STUDY; ENZYME SPECIFICITY; HYDROLYSIS; NONHUMAN; PROTEIN CLEAVAGE; PROTEIN FUNCTION; SEPARATION TECHNIQUE;

BACILLUS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DEOXYRIBONUCLEASES; DNA; ENDONUCLEASES; HYDROLYSIS; OLIGONUCLEOTIDES; POLY U; RIBONUCLEASES; RNA, BACTERIAL; SUBSTRATE SPECIFICITY;

BACILLUS FIRMUS;

EID: 79251582520     PISSN: 15257770     EISSN: 15322335     Source Type: Journal    
DOI: 10.1080/15257770.2010.541174     Document Type: Article

References (28)

1. Desai, N. A.; Shankar, V. Single-strand-specific nucleases. FEMS Microbiol Rev 2003, 26, 457-491.
2. Gerald, R.; Tilak, W. Yeast derived products. In Yeast technology, AVI, New York, 1991, pp. 369-412.
3. Qing, Y.; Shi, L. E.; Yu, Y.; Xing, T. Z.; Jian Shu, C. Production, purification and characterization of nuclease p1 from Penicillium citrinum. Process Biochem. 2006, 41, 1276-1281
4. Ashokkumar, P.; Muthu Manikandan, M.; Kannan, V. Optimization of nutrients for the production of RNase by Bacillus firmusVKPACU1 using response surface methodology. Biotechnol. Bioprocess Eng. 2010, 15(4), 641-650.
5. Apte, P. S.; Deshpande, M. V.; Shankar, V. Optimization of medium for extracellular nuclease formation from Rhizopus stolonifer. World. J. Microbiol. Biotechnol. 1993, 9, 205-209.
6. Ho, H. C.; Shiau, P. F.; Liu, F. C.; Chung, J. G.; Chen, L. Y. Purification, characterization and complete amino acid sequence of nuclease Cl from Cunninghamella echinulata var. echinulata. Eur. J. Biochem. 1998, 256, 112-118.
7. Sana, B.; Ghosh, D.; Saha, M.; Mukherjee, J. Purification and characterization of an extracellular, uracil specific ribonuclease from a Bizionia species isolated from the marine environment of the Sundarbans. Microbiol Res. 2008, 163(1), 31-38.
8. Rangarajan, E.; and Shankar, V. Extracellular nuclease from Rhizopus stolonifer: purification and characteristics of single strand preferential deoxyribonuclease activity. Biochim. Biophys. Acta 1999, 1473, 293-304.
9. Rangarajan, E. S. Purification and characteristics of extracellular nuclease from Rhizopus stolonifer. Ph. D Thesis, Pune, 2001.
10. Mikulski, A. J.; Laskowski, Sr., M. Mung bean nuclease. I. III. purification procedure and (3′) gmonophosphatase activity. J. Biol. Chem. 1970, 245, 5026-5031.
11. Desai, N. A.; Shankar, V. Single-strand-specific nuclease from Basidiobolus haptosporus (Nuclease Bh1). Sci. Res. Essays. 2007, 2(5), 139-146.
12. Desai, N. A.; Shankar, V. Nuclease Bh1 from Basidiobolus haptosporus: Characterization of the associated-adenylic acid preferential-ribonuclease activity. J. Biochem. Mol. Biol. Biophys. 2001, 5, 169-176.
13. Kuligowska, E.; Klarkowska, D.; Szarkowski, J. W. Purification and properties of endonuclease from wheat chloroplasts, specific for single-stranded DNA. Phytochem. 1988, 27, 1275-1279.
14. Monko, M.; Kuligowska, E.; Szarkowski, J. W.. A single-strand-specific nuclease from a fraction of wheat chloroplast stromal protein. Phytochem. 1994, 37, 301-305.
15. Siwecka, M. A.; Rytel, M.; Szarkowski, J. W. Purification and characterization of nuclease I associated with rye germ ribosomes. Acta Biochim Pol. 1989, 36, 45-62.
16. Przykorska, A. N.; Szarkowski, J. W. Single-strand-specific nuclease from the nucleoplasm of rye germ nuclei. Eur. J. Biochem. 1980, 108, 285-294.
17. Brown, P. H.; Ho, T. H. Biochemical properties and hormonal regulation of barley nuclease. Eur. J. Biochem. 1987, 168, 357-364.
18. Lee, S. Y.; Nakao, Y.; Bock, R. M.; The nucleases of yeast. II. Purification, properties and specificity of an endonuclease from yeast. Biochim. Biophys. Acta. 1968, 151, 126-136.
19. Sen, K.; Kawai, T.; Kurosawa, S. Mode of action of a nuclease from the fruit body of Flammulina velutipes. Agric. Biol. Chem. 1991, 55, 775-780.
20. Nestle, M.; Roberts, W. K. An Extracellular Nuclease from Serratia marcescens. I. Purification and some properties of the enzyme J. Biol. Chem. 1969, 244, 5213-5218.
21. Gray, H. B.; Winston, T. P.; Hodnett, J. L.; Legerski, R. J.; Nees, D. W.; Wei, C. F.; Robberson, D. L. The extracellular nuclease from Alteromonas espejiana: An enzyme highly specific for nonduplex structure in nominally duplex DNAs. In Gene amplification and analysis, Vol. 2, eds. J. G. Chirikjian and T. S. Papas, Elsevier/North Holland, New York, 1981, pp 169-203..
22. Holloman, W. K.; Rowe, T. C.; Rusche, J. R. Studies on nuclease α, from Ustilago maydis. J. Biol. Chem. 1981, 256, 5087-5094.
23. Ando, T. A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae. Biochim Biophys Acta. 1966, 18, 114(1), 158-168.
24. Fujimoto, M.; Kuninaka, A.; Yoshino, H. Substrate specificity of nuclease P1. Agric. Biol. Chem. 1974, 38, 1555-1561.
25. Fraser, M. J. Purification and properties of Neurospora crassa endo-exonuclease, an enzyme which can be converted to a single strand specific endonuclease. Methods Enzymol. 1980, 65, 255-263.
26. Desai, N. A.; Shankar, V. Purificaation and characterization of the single strand specific and guanylic acid-preferential deoxyribonuclease activity of the extracellular nuclease from Basidiobolus haptosporus. Eur. J. Biochem. 2000, 267, 5123-5135.
27. Laskowski Sr., M. Purification and properties of the mung bean nuclease. Methods Enzymol. 1980, 65, 263-275.
28. Kroeker, W. D.; Hanson, D. M.; Fairely, J. L. Activity of wheat seedling nuclease toward single-stranded nucleic acids. J. Biol. Chem. 1975, 250, 3767-3772.