Identification of critical residues of the mycobacterial dephosphocoenzyme a kinase by site-directed mutagenesis

PLoS ONE

Volumn 6, Issue 1, 2011, Pages

  Walia, Guneet ^a   Gajendar, Komatireddy ^b   Surolia, Avadhesha ^a,b  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b NATIONAL INSTITUTE OF IMMUNOLOGY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; ARGININE; ASPARAGINE; COENZYME A; DEPHOSPHOCOENZYME A KINASE; GLYCINE; LEUCINE; LYSINE; MAGNESIUM; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; DEPHOSPHO COENZYME A KINASE; DEPHOSPHO-COA KINASE; PHOSPHOTRANSFERASE;

ARTICLE; CATALYSIS; CIRCULAR DICHROISM; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENTHALPY; ENTROPY; ENZYME SUBSTRATE; HYDROPHOBICITY; MYCOBACTERIUM; NONHUMAN; NUCLEOTIDE BINDING SITE; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; THERMODYNAMICS; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; HUMAN; PROTEIN BINDING;

BACTERIAL PROTEINS; CATALYSIS; HUMANS; MUTAGENESIS, SITE-DIRECTED; MYCOBACTERIACEAE; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PROTEIN BINDING; SUBSTRATE SPECIFICITY; THERMODYNAMICS;

EID: 79251579267     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0015228     Document Type: Article

References (24)

1. Leonardi R, Zhang YM, Rock CO, Jackowski S (2005) Coenzyme A: back in action. Prog Lip Res 44: 125-153.
2. Murzin AG, Brenner SE, Hubbard T, Chothia C (1995) SCOP: a structural classification of proteins database for the investigation of sequences and structures. J Mol Biol 247: 536-540.
3. Seto A, Murayama K, Toyama M, Ebihara A, Makagawa N, et al. (2005) ATPinduced structural change of dephosphocoenzyme A kinase from Thermus thermophilus HB8. Proteins 58: 235-242.
4. O'Toole N, Barbosa JARG, Li Y, Jung LW, Matte A, et al. (2003) Crystal structure of a trimeric form of dephosphocoenzyme A kinase from Escherichia coli. Protein Sci 12: 327-36.
5. Obmolova G, Teplyakov A, Bonander N, Eisenstein E, Howard AJ, et al. (2001) Crystal structure of dephospho-coenzyme A kinase from Haemophilus influenzae. J Struct Biol 136: 119-25.
6. Walia G, Kumar P, Surolia A (2009) The Role of UPF0157 in the Folding of M. tuberculosis dephosphocoenzyme A kinase and the regulation of the latter by CTP. PLoS ONE 4(10): e7645. doi:10.1371/journal.pone.0007645.
7. Zhyvoloup A, Nemazanyy I, Panasyuk G, Valovka T, Fenton T, et al. (2003) Subcellular localization and regulation of coenzyme A synthase. J Biol Chem 278: 50316-50321.
8. Holm L, Sander C (1998) Touring protein fold space with Dali/FSSP. Nucl Acids Res. (1998) 26(1): 316-319.
9. Vonrhein C, Bönisch H, Schäfer G, Schulz GE (1998) The structure of a trimeric archaeal adenylate kinase. J Mol Biol (11): 167-179.
10. Kraft L, Sprenger GA, Lindqvis Y (2002) Conformational changes during the catalytic cycle of gluconate kinase as revealed by X-ray crystallography. J Mol Biol 318(4): 1057-1069.
11. Scheffzek K, Kliche W, Wiesmueller L, Reinstein J (1996) Crystal structure of the complex of UMP/CMP kinase from Dictyostelium discoideum and the bisubstrate inhibitor P1-(59-adenosyl) P5-(59-uridyl) pentaphosphate (UP5A) and Mg2+ at 2.2 A ° ́ implications for water-mediated specificity. Biochemistry 35: 9716-9727.
12. Jeyakanthan J, Kanaujia SP, Vasuki RC, Sekar K, Nakagawa N, et al. (2007) Crystal structure of thymidylate kinase (aq_969) from Aquifex aeolicus VF5. PDBTo be published.
13. Todd MJ, Gomez J (2001) Enzyme kinetics determined using calorimetry: A general assay for enzyme activity? Anal Biochem 296: 179-187.
14. Walker JE, Saraste M, Runswick MJ, Gay NJ (1982) Distantly related sequences in the a- and b-subunits of ATP synthase, myosin, kinases and other ATPrequiring enzymes and a common nucleotide binding fold. EMBO J; 1: 945-951.
15. Deyrup A, Krishnan S, Cockburn BN, Schwartz NB (1998) Deletion and Sitedirected Mutagenesis of the ATP-binding Motif (P-loop) in the bifunctional murine ATP-sulfurylase/adenosine 59-phosphosulfate kinase Enzyme. J Biol Chem 273: 9450-9456.
16. Saraste M, Sibbald PR, Wittinghofer A (1990) The P-loop -a common motif in ATP- and GTP-binding proteins. Trends Biochem Sci 15: 430-434.
17. Delepelaire P (1994) PrtD, the integral membrane ATP-binding cassette component of the Erwinia chrysanthemi metalloprotease secretion system, exhibits a secretion signal-regulated ATPase activity. J Biol Chem 269: 27952-27957.
18. Stephens K, Roush C, Nester E (1995) Agrobacterium tumefaciens VirB11 protein requires a consensus nucleotide-binding site for function in virulence. J Bacteriol 177: 27-36.
19. Driscoll WJ, Komatsu K, Strot CA (1995) Proposed active site domain in estrogen sulfotransferase as determined by mutational analysis. Proc Natl Acad Sci 92: 12328-12332.
20. 2+ Proteins. 32(3): 276-88.
21. Cantwell JS, Novak WR, Wang PF, McLeish MJ, Kenyon GL, et al. (2001) Mutagenesis of two acidic active site residues in human muscle creatine kinase: implications for the catalytic mechanism. Biochemistry 40(10): 3056-61.
22. Zhou G, Somasundaram T, Blanc E, Parthasarathy G, Ellington WR, et al. (1998) Transition state structure of arginine kinase: implications for catalysis of bimolecular reactions. Proc Natl Acad Sci 95: 8449-8454.
23. Bartlett G, Porter TC, Borkakoti N, Thornton JM (2002) Analysis of catalytic residues in enzyme active sites. J Mol Biol 324(1): 105-121.
24. Mishra P, Park PK, Drueckhammer DG (2001) Identification of yacE (coaE) as the structural gene for dephosphocoenzyme A kinase in Escherichia coli K-12. J Bact 183(9): 2774-8.