Application of quantitative PCR to the diagnosis and monitoring of Pseudomonas aeruginosa colonization in 5-18-year-old cystic fibrosis patients

Journal of Medical Microbiology

Volumn 60, Issue 2, 2011, Pages 157-161

  Billard Pomares, Typhaine ^a   Herwegh, Stéphanie ^a   Wizla Derambure, Nathalie ^a   Turck, Dominique ^a,b   Courcol, René ^a   Husson, Marie Odile ^a,b  

^a LILLE UNIVERSITY HOSPITAL   (France)

^b UNIV LILLE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CEFTAZIDIME; CIPROFLOXACIN; TOBRAMYCIN;

ADOLESCENT; ADULT; ARTICLE; BACTERIAL COLONIZATION; BACTERIUM CULTURE; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; CYSTIC FIBROSIS; DISEASE EXACERBATION; HUMAN; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTION; QUANTITATIVE ANALYSIS; SCHOOL CHILD; SPUTUM ANALYSIS;

ADOLESCENT; ANTI-BACTERIAL AGENTS; BACTERIOLOGICAL TECHNIQUES; CHILD; CHILD, PRESCHOOL; CYSTIC FIBROSIS; DRUG MONITORING; FEMALE; FOLLOW-UP STUDIES; HUMANS; MALE; PNEUMONIA, BACTERIAL; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; SENSITIVITY AND SPECIFICITY; SPUTUM; TIME FACTORS;

EID: 79251474054     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.023838-0     Document Type: Article

References (14)

1. Burns, J. L., Gibson, R. L., McNamara, S., Yim, D., Emerson, J., Rosenfeld, M., Hiatt, P., McCoy, K., Castile, R. & other authors (2001). Longitudinal assessment of Pseudomonas aeruginosa in young children with cystic fibrosis. J Infect Dis 183, 444-452.
2. Deschaght, P., De Baere, T., Van Simaey, L., Van Daele, S., De Baets, F., De Vos, D., Pirnay, J.-P. & Vaneechoutte, M. (2009). Comparison of the sensitivity of culture, PCR and quantitative realtime PCR for the detection of Pseudomonas aeruginosa in sputum of cystic fibrosis patients. BMC Microbiol 9, 244.
3. De Vos, D., Lim, A., Jr, Pirnay, J.-P., Struelens, M., Vandenvelde, C., Duinslaeger, L., Vanderkelen, A. & Cornelis, P. (1997). Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL. J Clin Microbiol 35, 1295-1299.
4. De Vos, D., De Chial, M., Cochez, C., Jansen, S., Tümmler, B., Meyer, J.-M. & Cornelis, P. (2001). Study of pyoverdine type and production by Pseudomonas aeruginosa isolated from cystic fibrosis patients: prevalence of type II pyoverdine isolates and accumulation of pyoverdine-negative mutations. Arch Microbiol 175, 384-388.
5. Frederiksen, B., Koch, C. & Høiby, N. (1997). Antibiotic treatment of initial colonization with Pseudomonas aeruginosa postpones chronic infection and prevents deterioration of pulmonary function in cystic fibrosis. Pediatr Pulmonol 23, 330-335. (Pubitemid 27225208)
6. Frederiksen, B., Koch, C. & Høiby, N. (1999). Changing epidemiology of Pseudomonas aeruginosa infection in Danish cystic fibrosis patients (1974-1995). Pediatr Pulmonol 28, 159-166.
7. Høiby, N., Frederiksen, B. & Pressler, T. (2005). Eradication of early Pseudomonas aeruginosa infection. J Cyst Fibros 4, 49-54.
8. Karpati, F. & Jonasson, J. (1996). Polymerase chain reaction for the detection of Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Burkholderia cepacia in sputum of patients with cystic fibrosis. Mol Cell Probes 10, 397-403.
9. Pirnay, J.-P., De Vos, D., Duinslaeger, L., Reper, P., Vandenvelde, C., Cornelis, P. & Vanderkelen, A. (2000). Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid 'real-time' polymerase chain reaction. Crit Care 4, 255-261.
10. Rosenfeld, M., Emerson, J., Williams-Warren, J., Pepe, M., Smith, A., Montgomery, A. B. & Ramsey, B. W. (2001a). Defining a pulmonary exacerbation in cystic fibrosis. J Pediatr 139, 359-365.
11. Rosenfeld, M., Gibson, R. L., McNamara, S., Emerson, J., Burns, J. L., Castile, R., Hiatt, P., McCoy, K., Wilson, C. B. & other authors (2001b). Early pulmonary infection, inflammation, and clinical outcomes in infants with cystic fibrosis. Pediatr Pulmonol 32, 356-366.
12. Rosenfeld, M., Ramsey, B. W. & Gibson, R. L. (2003). Pseudomonas acquisition in young patients with cystic fibrosis: pathophysiology, diagnosis, and management. Curr Opin Pulm Med 9, 492-497. (Pubitemid 37268213)
13. van Belkum, A., Renders, N. H. M., Smith, S., Overbeek, S. E. & Verbrugh, H. A. (2000). Comparison of conventional and molecular methods for the detection of bacterial pathogens in sputum samples from cystic fibrosis patients. FEMS Immunol Med Microbiol 27, 51-57.
14. Xu, J., Moore, J. E., Murphy, P. G., Millar, B. C. & Elborn, J. S. (2004). Early detection of Pseudomonas aeruginosa: comparison of conventional versus molecular (PCR) detection directly from adult patients with cystic fibrosis (CF). Ann Clin Microbiol Antimicrob 3, 21.