Identification of cytomegalovirus (CMV)pp65 antigen-specific human monoclonal antibodies using single B cell-based antibody gene cloning from melanoma patients

Immunology Letters

Volumn 135, Issue 1-2, 2011, Pages 64-73

  Iizuka, Akira ^a   Komiyama, Masaru ^a   Tai, Sachiko ^a   Oshita, Chie ^a   Kurusu, Ayumi ^a   Kume, Akiko ^a   Ozawa, Kazumichi ^a   Nakamura, Yoji ^a   Ashizawa, Tadashi ^a   Yamamoto, Akifumi ^c   Yamazaki, Naoya ^b   Yoshikawa, Shusuke ^a   Kiyohara, Yoshio ^a   Yamaguchi, Ken ^a   Akiyama, Yasuto ^a  

^a SHIZUOKA CANCER CENTER   (Japan)

^b NATIONAL CANCER CENTER HOSPITAL   (Japan)

^c SAITAMA MEDICAL UNIVERSITY   (Japan)

Author keywords

Cytomegalovirus pp65 antigen; Direct antibody gene cloning; Human IgG ELISA; Human monoclonal antibody; Single B cell

Indexed keywords

BETA ACTIN; CYTOMEGALOVIRUS ANTIGEN PP65; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; MESSENGER RNA; MONOCLONAL ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY AFFINITY; ANTIBODY DETECTION; ANTIBODY PRODUCTION; ANTIBODY RESPONSE; ANTIBODY SPECIFICITY; ANTIBODY TITER; ARTICLE; B LYMPHOCYTE; CONTROLLED STUDY; COPY NUMBER VARIATION; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENE SEQUENCE; GENE TECHNOLOGY; HUMAN; HUMAN CELL; IMMUNOREACTIVITY; MELANOMA; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANTIBODIES, MONOCLONAL; ANTIBODIES, VIRAL; B-LYMPHOCYTES; CLONING, MOLECULAR; CYTOMEGALOVIRUS; HUMANS; IMMUNOGLOBULIN G; MELANOMA; PHOSPHOPROTEINS; SINGLE-CHAIN ANTIBODIES; VIRAL MATRIX PROTEINS;

EID: 78751700999     PISSN: 01652478     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.imlet.2010.09.013     Document Type: Article

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