Development of an in situ assay for simultaneous detection of the genomic and replicative form of PCV2 using padlock probes and rolling circle amplification

Virology Journal

Volumn 8, Issue , 2011, Pages

  Henriksson, Sara ^a   Blomström, Anne Lie ^b   Fuxler, Lisbeth ^b   Fossum, Caroline ^b   Berg, Mikael ^b   Nilsson, Mats ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTIC METHOD; ARTICLE; ASSAY; CIRCOVIRUS; GENE AMPLIFICATION; MOLECULAR PROBE; NONHUMAN; VIRUS GENOME; VIRUS REPLICATION; ANIMAL; ANIMAL DISEASE; CELL LINE; CELL NUCLEUS; CIRCOVIRIDAE INFECTION; CYTOPLASM; GENETICS; ISOLATION AND PURIFICATION; LYMPH NODE; METHODOLOGY; NUCLEIC ACID AMPLIFICATION; OLIGONUCLEOTIDE PROBE; PHYSIOLOGY; SWINE; SWINE DISEASE; VIROLOGY;

DNA VIRUSES; PORCINE CIRCOVIRUS; PORCINE CIRCOVIRUS 2; SUIDAE;

VIRUS DNA;

ANIMALS; CELL LINE; CELL NUCLEUS; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; CYTOPLASM; DNA, VIRAL; LYMPH NODES; NUCLEIC ACID AMPLIFICATION TECHNIQUES; OLIGONUCLEOTIDE PROBES; SWINE; SWINE DISEASES; VIROLOGY; VIRUS REPLICATION;

EID: 78751647432     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/1743-422X-8-37     Document Type: Article

References (27)

1. Padlock probes: circularizing oligonucleotides for localized DNA detection. M Nilsson, et al. Scie (Pubitemid 24325689)
2. Rolling replication of short DNA circles. A Fire SQ Xu, Proc. Natl. Acad. Sci. USA 1995 92 4641 4645 10.1073/pnas.92.10.4641
3. Mutation detection and single-molecule counting using isothermal rolling-circle amplification. PM Lizardi X Huang, et al. Nat Genet 1998 19 3 225 232 10.1038/898 9662393 (Pubitemid 28309332)
4. BlobFinder, a tool for fluorescence microscopy image cytometry. A Allalou C Wahlby, Comput Methods Programs Biomed 2009 94 1 58 65 10.1016/j.cmpb.2008. 08.006 18950895
5. In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes. C Larsson J Koch, et al. Nat Methods 2004 1 3 227 232 10.1038/nmeth723 15782198
6. In situ detection and genotyping of individual mRNA molecules. C Larsson I Grundberg O Söderberg M Nilsson, Nat Methods 2010 7 5 395 10.1038/nmeth.1448 20383134
7. In situ detection of Anaplasma spp. by DNA target-primed rolling-circle amplification of a padlock probe and intracellular colocalization with immunofluorescently labeled host cell von Willebrand factor. HL Wamsley AF Barbet, J Clin Microbiol 2008 46 7 2314 9 10.1128/JCM.02197-07 18495855
8. Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe. GM Allan F McNeilly S Kennedy B Daft EG Clarke JA Ellis DM Haines BM Meehan BM Adair, J Vet Diagn Invest 1998 10 1 3 10 9526853 (Pubitemid 128656696)
9. Characterization of novel circovirus DNAs associated with wasting syndromes in pigs. BM Meehan F McNeilly D Todd S Kennedy VA Jewhurst JA Ellis LE Hassard EG Clark DM Haines GM Allan, J Gen Virol 1998 79 Pt 9 2171 9 9747726 (Pubitemid 28423412)
10. Porcine circoviruses: a review. GM Allan JA Ellis, J Vet Diagn Invest 2000 12 1 3 14 Review. Advisory Committee on Genetic Modification: Annual Report. London; 1999 10690769
11. Porcine circovirus diseases. J Segalés GM Allan M Domingo, Anim Health Res Rev 2005 6 2 119 42 16583778
12. Postweaning multisystemic wasting syndrome: a review of aetiology, diagnosis and pathology. C Chae, Vet J 2004 168 1 41 9 15158207 (Pubitemid 38684203)
13. Replication of porcine circoviruses. F Faurez D Dory B Grasland A Jestin, Virol J 2009 18 6 60 10.1186/1743-422X-6-60
14. Glycosylases and AP-cleaving enzymes as a general tool for probe-directed cleavage of ssDNA targets. WM Howell I Grundberg M Faryna U Landegren M Nilsson, Nucleic Acids Res 2010 38 7 99 10.1093/nar/gkp1238 20081204
15. Subcellular immunolocalization of porcine circovirus type 2 (PCV2) in lymph nodes from pigs with post-weaning multisystemic wasting syndrome (PMWS). C Rodríguez-Carĩo A Snchez-Chardi J Segalés, J Comp Pathol 2010 142 291 9 20096850
16. In vitro studies on the infection and replication of porcine circovirus type 2 in cells of the porcine immune system. DF Gilpin K McCullough BM Meehan F McNeilly I McNair LS Stevenson JC Foster JA Ellis S Krakowka BM Adair GM Allan, Vet Immunol Immunopathol 2003 94 3-4 149 61 10.1016/S0165-2427(03)00087-4 12909411 (Pubitemid 37297421)
17. Dendritic cells harbor infectious porcine circovirus type 2 in the absence of apparent cell modulation or replication of the virus. IE Vincent CP Carrasco B Herrmann BM Meehan GM Allan A Summerfield KC McCullough, J Virol 2003 77 24 13288 300 10.1128/JVI.77.24.13288-13300.2003 14645585 (Pubitemid 37494324)
18. Ultrastructural findings in lymph nodes from pigs suffering from naturally occurring postweaning multisystemic wasting syndrome. C Rodriguez-Carĩo J Segalés, Vet Pathol 2009 46 4 729 35 Epub 2009 Mar 9 19276043
19. Porcine Circovirus Type 2 Morphogenesis in a Clone Derived from the L35 Lymphoblastoid Cell Line. C Rodríguez-Carĩo C Duffy A Snchez-Chardi F McNeilly GM Allan J Segalés, J Comp Pathol 2010 2010 Aug 25
20. Comparative study of in situ hybridization and immunohistochemistry for the detection of porcine circovirus 2 in formalin-fixed, paraffin-embedded tissues. D Kim Y Ha YH Lee S Chae K Lee K Han J Kim JH Lee SH Kim KK Hwang C Chae, J Vet Med Sci 2009 71 7 1001 4 10.1292/jvms.71.1001 19652494
21. Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue. SD Sorden PA Harms P Nawagitgul D Cavanaugh PS Paul, J Vet Diagn Invest 1999 11 6 528 30 Advisory Committee on Genetic Modification: Annual Report. London; 1999 12968735 (Pubitemid 129693084)
22. A comparison of in situ hybridization and immunohistochemistry for the detection of a new porcine circovirus in formalin-fixed tissues from pigs with post-weaning multisystemic wasting syndrome (PMWS). F McNeilly S Kennedy D Moffett BM Meehan JC Foster EG Clarke JA Ellis DM Haines BM Adair GM Allan, J Virol Methods 1999 80 2 123 8 10.1016/S0166-0934(99)00043-9 10471021
23. A novel method for the detection of porcine circovirus type 2 replicative double stranded viral DNA and nonreplicative single stranded viral DNA in tissue sections. A Hamberg S Ringler S Krakowka, J Vet Diagn Invest 2007 19 135 141 17402607
24. Studies of porcine circovirus type 2, porcine boca-like virus and torque teno virus indicate the presence of multiple viral infections in postweaning multisystemic wasting syndrome pigs. A-L Blomström C Fossum S Bélak P Wallgren M Berg, Virus Research 2010 152 59 64 20542066
25. Phylogenetic analysis of porcine circovirus type 2 (PCV2) pre- and post-epizootic postweaning multisystemic wasting syndrome (PMWS). S Timmusk P Wallgren IM Brunborg FH Wikström G Allan B Meehan M McMenamy F McNeilly L Fuxler K Belk D Pdersoo T Saar M Berg C Fossum, Virus Genes 2008 36 509 520 10.1007/s11262-008-0217-1 18343985
26. Experimental reproduction of postweaning multisystemic wasting syndrome (PMWS) in pigs in Sweden and Denmark with a Swedish isolate of porcine circovirus type 2. F Hasslung P Wallgren AS Ladekjaer-Hansen A Bøtner J Nielsen E Wattrang GM Allan F McNeilly J Ellis S Timmusk K Belk T Segall L Melin M Berg C Fossum, Vet Microbiol 2005 106 49 60 10.1016/j.vetmic.2004.12.011 15737473 (Pubitemid 40298858)
27. Dynamics of serum antibodies to and load of porcine circovirus type 2 (PCV2) in pigs in three finishing herds, affected or not by postweaning multisystemic wasting syndrome. IM Brunborg C Fossum B Lium G Blomqvist E Merlot A Jørgensen L Eliasson-Selling E Rimstad CM Jonassen P Wallgren, Acta Vet Scand 2010 52 22 10.1186/1751-0147-52-22 20302632