A Novel Method for the Detection of Porcine Circovirus Type 2 Replicative Double Stranded Viral DNA and Nonreplicative Single Stranded Viral DNA in Tissue Sections

Journal of Veterinary Diagnostic Investigation

Volumn 19, Issue 2, 2007, Pages 135-141

  Hamberg, Alexander ^a   Ringler, Susan ^a   Krakowka, Steven ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

Immunohistochemistry; in situ hybridization; PCV2; porcine circovirus

Indexed keywords

CIRCOVIRUS; PORCINE CIRCOVIRUS; PORCINE CIRCOVIRUS 2; SUIDAE; SUS;

SINGLE STRANDED DNA; VIRUS DNA;

ANIMAL; ANIMAL DISEASE; ARTICLE; CIRCOVIRIDAE; GENETICS; IN SITU HYBRIDIZATION; ISOLATION AND PURIFICATION; PATHOLOGY; PHYSIOLOGY; SWINE; SWINE DISEASE; VIROLOGY; VIRUS INFECTION; VIRUS REPLICATION;

ANIMALS; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; DNA, SINGLE-STRANDED; DNA, VIRAL; IN SITU HYBRIDIZATION; SWINE; SWINE DISEASES; VIRUS REPLICATION;

EID: 34447317872     PISSN: 10406387     EISSN: 19434936     Source Type: Journal    
DOI: 10.1177/104063870701900201     Document Type: Article

References (20)

1. Allan GM,Ellis JA.Porcine circoviruses: A review.J Vet Diagn Invest. 2000;12:3-14.
2. Allan GM,McNeilly E,Kennedy S, et al.PCV-2-associated PDNS in Northern Ireland in 1990. Porcine dermatitis and nephropathy syndrome.Vet Rec. 2000;146:711-712.
3. Allan GM,Smyth JA,Todd D,McNulty MS.In situ hybridization for the detection of chicken anemia virus in formalin-fixed, paraffin-embedded sections.Avian Dis. 1993;37:177-182.
4. Altschul SF,Madden TL,Schaffer AA, et al.Gapped BLAST and PSI-BLAST: A new generation of protein database search programs.Nucleic Acids Res. 1997;25:3389-3402.
5. Chae C.Postweaning multisystemic wasting syndrome: A review of aetiology, diagnosis and pathology.Vet J. 2004;168:41-49.
6. Crowther RA,Berriman JA,Curran WL, et al.Comparison of the structures of three circoviruses: Chicken anemia virus, porcine circovirus type 2, and beak and feather disease virus.J Virol. 2003;77:13036-13041.
7. Fauquet CInternational Committee on Taxonomy of Viruses, International Union of Microbiological Societies. Virology Division. Virus Taxonomy: Classification and Nomenclature of Viruses: Eighth Report of the International Committee on the Taxonomy of Viruses. Boston: Elsevier Academic Press; 2005:327-330.
8. Frankfurt OS.Detection of apoptosis in leukemic and breast cancer cells with monoclonal antibody to single-stranded DNA.Anticancer Res. 1994;14:1861-1869.
9. Frankfurt OS,Robb JA,Sugarbaker EV,Villa L.Monoclonal antibody to single-stranded DNA is a specific and sensitive cellular marker of apoptosis.Exp Cell Res. 1996;226:387-397.
10. Krakowka S,Ellis J,McNeilly F, et al.Features of cell degeneration and death in hepatic failure and systemic lymphoid depletion characteristic of porcine circovirus-2-associated postweaning multisystemic wasting disease.Vet Pathol. 2004;41:471-481.
11. Krakowka S,Ellis J,McNeilly F, et al.Features of porcine circovirus-2 disease: Correlations between lesions, amount and distribution of virus, and clinical outcome.J Vet Diagn Invest. 2005;17:213-222.
12. Krakowka S,Ellis JA,McNeilly F, et al.Immunologic features of porcine circovirus type 2 infection.Viral Immunol. 2002;15:567-582.
13. Mankertz A,Caliskan R,Hattermann K, et al.Molecular biology of porcine circovirus: Analyses of gene expression and viral replication.Vet Microbiol. 2004;98:81-88.
14. Mankertz A,Persson F,Mankertz J, et al.Mapping and characterization of the origin of DNA replication of porcine circovirus.J Virol. 1997;71:2562-2566.
15. McNeilly F,Kennedy S,Moffett D, et al.A comparison of in situ hybridization and immunohistochemistry for the detection of a new porcine circovirus in formalin-fixed tissues from pigs with post-weaning multisystemic wasting syndrome (PMWS).J Virol Methods. 1999;80:123-128.
16. McNeilly F,McNair I,Mackie DP, et al.Production, characterization and applications of monoclonal antibodies to porcine circovirus 2.Arch Virol. 2001;146:909-922.
17. Meehan BM,Creelan JL,McNulty MS,Todd D.Sequence of porcine circovirus DNA: Affinities with plant circoviruses.J Gen Virol. 1997;78:221-227.
18. Meehan BM,McNeilly F,McNair I, et al.Isolation and characterization of porcine circovirus 2 from cases of sow abortion and porcine dermatitis and nephropathy syndrome.Arch Virol. 2001;146:835-842.
19. Meehan BM,McNeilly F,Todd D, et al.Characterization of novel circovirus DNA associated with wasting syndromes in pigs.J Gen Virol. 1998;79:2171-2179.
20. Meerts P,Misinzo G,McNeilly F,Nauwynck HJ.Replication kinetics of different porcine circovirus 2 strains in PK-15 cells, fetal cardiomyocytes and macrophages.Arch Virol. 2005;150:427-441.