Human LDL core cholesterol ester packing: Three-dimensional image reconstruction and SAXS simulation studies

Journal of Lipid Research

Volumn 52, Issue 2, 2011, Pages 256-262

  Liu, Yuhang ^a   Luo, Dong ^a   Atkinson, David ^a  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Atherosclerosis; Electron cryo microscopy; Low density lipoprotein; Phase transition; Small angle x ray scattering

Indexed keywords

CHOLESTEROL ESTER; LIPID; LOW DENSITY LIPOPROTEIN;

ARTICLE; CONFORMATIONAL TRANSITION; CRYOPRESERVATION; ELECTRON MICROSCOPY; IMAGE RECONSTRUCTION; MEMBRANE STRUCTURE; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN STRUCTURE; STRUCTURE ANALYSIS; SURFACE PROPERTY; THREE DIMENSIONAL IMAGING; X RAY CRYSTALLOGRAPHY;

CHOLESTEROL ESTERS; CHOLESTEROL, LDL; CRYOELECTRON MICROSCOPY; HUMANS; LIPOPROTEINS, LDL; PHASE TRANSITION; SCATTERING, SMALL ANGLE; X-RAY DIFFRACTION;

EID: 78751559413     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.M011569     Document Type: Article

References (25)

1. Hevonoja, T., M. O. Pentikainen, M. T. Hyvonen, P. T. Kovanen, and M. Ala-Korpela. 2000. Structure of low density lipoprotein (LDL) particles: basis for understanding molecular changes in modified LDL. Biochim. Biophys. Acta. 1488:189-210.
2. Mahley, R. W., T. L. Innerarity, S. C. Rall, Jr., and K. H. Weisgraber. 1984. Plasma lipoproteins: apolipoprotein structure and function. J. Lipid Res. 25:1277-1294.
3. Brown, M. S., and J. L. Goldstein. 1974. Familial hypercholesterolemia: defective binding of lipoproteins to cultured fibroblasts associated with impaired regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity. Proc. Natl. Acad. Sci. USA. 71:788-792.
4. Brown, M. S., and J. L. Goldstein. 1979. Receptor-mediated endocytosis: insights from the lipoprotein receptor system. Proc. Natl. Acad. Sci. USA. 76:3330-3337.
5. Goldstein, J. L., and M. S. Brown. 1976. The LDL pathway in human fibroblasts: a receptor-mediated mechanism for the regulation of cholesterol metabolism. Curr. Top. Cell. Regul. 11:147-181.
6. Watkins, H., and M. Farrall. 2006. Genetic susceptibility to coronary artery disease: from promise to progress. Nat. Rev. Genet. 7:163-173. (Pubitemid 43361528)
7. Atkinson, D., R. J. Deckelbaum, D. M. Small, and G. G. Shipley. 1977. Structure of human plasma low-density lipoproteins: molecular organization of the central core. Proc. Natl. Acad. Sci. USA. 74:1042-1046.
8. Deckelbaum, R. J., G. G. Shipley, and D. M. Small. 1977. Structure and interactions of lipids in human plasma low density lipoproteins. J. Biol. Chem. 252:744-754.
9. Laggner, P., G. M. Kostner, G. Degovics, and D. L. Worcester. 1984. Structure of the cholesteryl ester core of human plasma low density lipoproteins: selective deuteration and neutron small-angle scattering. Proc. Natl. Acad. Sci. USA. 81:4389-4393.
10. Schuster, B., R. Prassl, F. Nigon, M. J. Chapman, and P. Laggner. 1995. Core lipid structure is a major determinant of the oxidative resistance of low density lipoprotein. Proc. Natl. Acad. Sci. USA. 92:2509-2513.
11. Prassl, R., B. Schuster, P. M. Abuja, M. Zechner, G. M. Kostner, and P. Laggner. 1995. A comparison of structure and thermal behavior in human plasma lipoprotein(a) and low-density lipoprotein. Calorimetry and small-angle X-ray scattering. Biochemistry. 34:3795-3801.
12. Liu, Y., and D. Atkinson. 2010. Enhancing the contrast of apoB to locate the surface components in the 3D density map of human LDL. J. Mol. Biol. 405:274-283.
13. Orlova, E. V., M. B. Sherman, W. Chiu, H. Mowri, L. C. Smith, and A. M. Gotto, Jr. 1999. Three-dimensional structure of low density lipoproteins by electron cryomicroscopy. Proc. Natl. Acad. Sci. USA. 96:8420-8425.
14. Ren, G., G. Rudenko, S. J. Ludtke, J. Deisenhofer, W. Chiu, and H. J. Pownall. 2010. Model of human low-density lipoprotein and bound receptor based on cryoEM. Proc. Natl. Acad. Sci. USA. 107:1059-1064.
15. Coronado-Gray, A., and R. van Antwerpen. 2005. Lipid composition influences the shape of human low density lipoprotein in vitreous ice. Lipids. 40:495-500. (Pubitemid 40911449)
16. Sherman, M. B., E. V. Orlova, G. L. Decker, W. Chiu, and H. J. Pownall. 2003. Structure of triglyceride-rich human low-density lipoproteins according to cryoelectron microscopy. Biochemistry. 42:14988-14993.
17. Prassl, R., M. Pregetter, H. Amenitsch, M. Kriechbaum, R. Schwarzenbacher, J. M. Chapman, and P. Laggner. 2008. Low density lipoproteins as circulating fast temperature sensors. PLoS ONE. 3:e4079.
18. Jayaraman, S., D. Gantz, and O. Gursky. 2005. Structural basis for thermal stability of human low-density lipoprotein. Biochemistry. 44:3965-3971. (Pubitemid 40358048)
19. Tang, G., L. Peng, P. R. Baldwin, D. S. Mann, W. Jiang, I. Rees, and S. J. Ludtke. 2007. EMAN2:an extensible image processing suite for electron microscopy. J. Struct. Biol. 157:38-46.
20. Ludtke, S. J., P. R. Baldwin, and W. Chiu. 1999. EMAN:semiautomated software for high-resolution single-particle reconstructions. J. Struct. Biol. 128:82-97.
21. Goddard, T. D., C. C. Huang, and T. E. Ferrin. 2005. Software extensions to UCSF chimera for interactive visualization of large molecular assemblies. Structure. 13:473-482.
22. Schneidman-Duhovny, D., M. Hammel, and A. Sali. 2010. FoXS: a web server for rapid computation and fitting of SAXS profiles. Nucleic Acids Res. 38 ( Suppl ):W540-544.
23. Small, D. M. 1986. The physical chemistry of lipids from alkanes to phospholipids. In Handbook of Lipid Research. D. J. Hanahan, editor. Plenum, New York. 475-522.
24. Siegel, D. P., W. J. Green, and Y. Talmon. 1994. The mechanism of lamellar-to-inverted hexagonal phase transitions: a study using temperature-jump cryo-electron microscopy. Biophys. J. 66:402-414.
25. Burton, A. 1935. Human calorimetry: the average temperature of the tissues of the body. J. Nutr. 9:261-280.