Direct diagnosis of Leishmania species on serosity materials punctured from cutaneous leishmaniasis patients using PCR-RFLP

Journal of Clinical Laboratory Analysis

Volumn 25, Issue 1, 2011, Pages 20-24

  Hajjaran, Homa ^a   Vasigheh, Farzaneh ^a   Mohebali, Mehdi ^a   Rezaei, Sasan ^a   Mamishi, Setareh ^b   Charedar, Soroure ^a  

^a TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Cutaneous leishmaniasis; Human; Identification; Lesion serosity material; PCR RFLP

Indexed keywords

INTERNAL TRANSCRIBED SPACER 1;

ADULT; ARTICLE; CONTROLLED STUDY; FEMALE; HUMAN; IRAN; LEISHMANIA; LEISHMANIA INFANTUM; LEISHMANIA MAJOR; LEISHMANIA TROPICA; MAJOR CLINICAL STUDY; MALE; MICROSCOPY; NONHUMAN; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SKIN LEISHMANIASIS;

ADOLESCENT; ADULT; AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS; FEMALE; HUMANS; LEISHMANIA; LEISHMANIA MAJOR; LEISHMANIASIS, CUTANEOUS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; SENSITIVITY AND SPECIFICITY; SKIN; YOUNG ADULT;

BACTERIA (MICROORGANISMS); LEISHMANIA MAJOR;

EID: 78751520033     PISSN: 08878013     EISSN: 10982825     Source Type: Journal    
DOI: 10.1002/jcla.20377     Document Type: Article

References (22)

1. Desjeux P. The increase in risk factors for leishmaniasis worldwide. Trans R Soc Trop Med Hyg 2001;95:239-243.
2. Alvar J, Croft S, Olliaro P. Chemotherapy in the treatment and control of leishmaniasis. Adv Parasitol 2006;61:223-274.
3. Vega Lopez F. Diagnosis of cutaneous leishmaniasis. Curr Opin Infect Dis 2003;16:97-101.
4. Bensoussan E, Nasereddin A, Jonas F, Schnur FL, Jaffe LC. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J Clin Microbiol 2006;44:1435-1439.
5. Profeta MZ, Rotondo da Silva A, Oliveira FS, Caligiorne BR, Oliveira E, Rabello A. Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis. Mem Inst Oswaldo Cruz Rio de Janeiro 2009;104:62-66.
6. Camila Indiani O, Fica BA, Oliveira F, et al Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of american cutaneous leishmaniasis. Clin Infect Dis 2003;37:149-153.
7. Lemrani M, Hamdi S, Laamrani A, Hassar M. PCR detection of Leishmania in skin biopsies. J Infect Developing Countries 2009;3:115-122.
8. Richard R, Jean-Claude D. Molecular diagnosis of leishmaniasis: Current status and future applications. J Clin Microbiol 2006;45:21-25.
9. Blum J, Desjeux P, Schwartz E, Beck B, Hatz C. Treatment of cutaneous leishmaniasis among travelers. J Antimicrob Chemother 2004;53:158-166.
10. Schonian G, Nasereddin A, Dinse N, et al. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 2003;47:349-358.
11. Kazemi-Rad E, Mohebali M, Hajjaran H, Rezaei S, Mamishi S. Diagnosis and characterization of Leishmania species in Giemsa-stained slides by PCR-RFLP. Iranian J Publ Health 2008;37:54-60.
12. Altman DG. Practical Statistics for Medical Research. London, UK: Chapman & Hall, 2001.
13. Bensoussan E, Nasereddin A, Jonas F, Schnur LF, Jaffe CL. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J Clin Microbiol 2006;44:1435-1439.
14. Herwaldt BL. Leishmaniasis. Lancet 1999;354:1191-1199.
15. Andresen KA, El-Hassan AM, Ismail A, Dafalla M, Theander TG, Kharazmi A. Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: A comparison with direct microscopy of smears and sections from lesions. Trans R Soc Trop Med Hyg 1996;90:133-135.
16. Quispe TKW, Ying X, Dedet JP, Rijal S, De Bolle X, Dujardin JC. Antigen genes for molecular epidemiology of leishmaniasis: Polymorphism of cysteine proteinase B and surface metalloprotease glycoprotein 63 in the Leishmania donovani complex. J Infect Dis 2004;189:1035-1043.
17. Garcia AL, Kindt A, Quispe-Tintya KW, et al American tegumentary leishmaniasis: Direct species identification of Leishmania in non-invasive clinical samples. Trans R Soc Trop Med Hyg 2007;101:368-371.
18. Gadisa E, Genetu A, Kuru T, et al. Leishmania (Kinetoplastida): Species typing with isoenzyme and PCR-RFLP from cutaneous leishmaniasis patients in Ethiopia. Exp Parasitol 2007;115:339-343.
19. Zemanova E, Jirku M, Mauricio LI, Hora KA, Miles AM, Julius Lukes J. The Leishmania donovani complex: Genotypes of five metabolic enzymes (ICD, ME, MPI, G6PDH, and FH), new targets for multilocus sequence typing. Int J Parasitol 2007;37:149-160.
20. Kumar R, Bumb R, Ansari AN, Mehta R, Salotra P. Cutaneous leishmaniasis caused by Leishmania tropica in Bikaner, India: Parasite identification and characterization using molecular and immunological tools. Am J Trop Med Hyg 2007;76:896-901.
21. Matsumoto T, Hashiguchi E, Gomez A, et al. Comparison of PCR results using scrape/exudate, syringe-sucked fluid and biopsy samples for diagnosis of cutaneous leishmaniasis in Ecuador. Trans R Soc Trop Med Hyg 1999;93:606-607.
22. Rotureau B, Ravel C, Couppie P, et al. Use of PCR-restriction fragment length polymorphism analysis to identify the main new world Leishmania species and analyze their taxonomic properties and polymorphism by application of the assay to clinical samples. J Clin Microbiol 2006;44:459-467.