Comparison of PCR results using scrape/exudate, syringe-sucked fluid and biopsy samples for diagnosis of cutaneous leishmaniasis in Ecuador

Transactions of the Royal Society of Tropical Medicine and Hygiene

Volumn 93, Issue 6, 1999, Pages 606-607

  Matsumoto, T ^a   Hashiguchi, Y ^b   Gomez, E A ^c   Calvopina M H ^d   Nonaka, S ^e   Saya, H ^f   Mimori, T ^f  

^a Ginkyo College of Medical Science   (Japan)

^b KOCHI MEDICAL SCHOOL   (Japan)

^c UNIVERSIDAD CATÓLICA DE SANTIAGO DE GUAYAQUIL   (Ecuador)

^d UNIVERSIDAD CENTRAL DEL ECUADOR   (Ecuador)

^e UNIVERSITY OF THE RYUKYUS   (Japan)

^f KUMAMOTO UNIVERSITY   (Japan)

Author keywords

Cutaneous leishmaniasis; Diagnosis; Ecuador; Polymerase chain reaction; Scrape exudate sample

Indexed keywords

ADOLESCENT; ADULT; ARTICLE; BODY FLUID; CLINICAL ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; ECUADOR; FEMALE; HUMAN; INTERMETHOD COMPARISON; MALE; POLYMERASE CHAIN REACTION; SAMPLING; SCHOOL CHILD; SKIN BIOPSY; SKIN LEISHMANIASIS;

EID: 0033375090     PISSN: 00359203     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0035-9203(99)90065-2     Document Type: Article

References (4)

1. Andresen, K., Gaafar, A., El-Hassan, A. M., Ismail, A., Dafalla, M., Theander, T. G. & Kharazmi, A. (1996). Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions. Transactions of the Royal Society of Tropical Medicine and Hygiene, 90, 133-135.
2. Belli, A., Rodriguez, B., Aviles, H. & Harris, E. (1998). Simplified polymerase chain reaction of New World Leishmania in clinical specimens of cutaneous leishmaniasis. American Journal of Tropical Medicine and Hygiene, 58, 102-109.
3. De Brujin, M. H. L., Labrada, L. A., Smyth, A. J., Santrich, C. & Barker, D. C. (1993). A comparative study of diagnosis by the polymerase chain reaction and by current clinical methods using biopsies from Colombian patients with suspected leishmaniasis. Tropical Medicine and Parasitology, 44, 201-207.
4. Mimori, T., Sasaki, J., Nakata, M., Gomez, E. A., Uezato, H., Nonaka, S., Hashiguchi, Y., Furuya, M. & Saya, H. (1998). Rapid identification of Leishmania species from formalinfixed biopsy samples by polymorphism-specific polymerase chain reaction. Gene, 210, 179-186.