Macroscale spatial variation in chronic wound microbiota: A cross-sectional study

Wound Repair and Regeneration

Volumn 19, Issue 1, 2011, Pages 80-88

  Price, Lance B ^a   Liu, Cindy M ^a,b   Frankel, Yelena M ^c   Melendez, Johan H ^c   Aziz, Maliha ^a   Buchhagen, Jordan ^a   Contente Cuomo, Tania ^a   Engelthaler, David M ^a   Keim, Paul S ^a,b   Ravel, Jacques ^d   Lazarus, Gerald S ^c   Zenilman, Jonathan M ^c  

^a TRANSLATIONAL GENOMICS RESEARCH INSTITUTE   (United States)

^b NORTHERN ARIZONA UNIVERSITY   (United States)

^c DEPARTMENT OF PATHOLOGY   (United States)

^d UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; AQUACEL AG; CIPROFLOXACIN; COLLAGEN; COTRIMOXAZOLE; MESOGLYCAN; RNA 16S; SULFADIAZINE SILVER; UNCLASSIFIED DRUG;

ADULT; AGED; ANTIBIOTIC THERAPY; ARTICLE; BACTERIAL INFECTION; BACTERIUM CULTURE; CHRONIC DISEASE; CLINICAL ARTICLE; CROSS-SECTIONAL STUDY; DECUBITUS; DIABETES MELLITUS; ECOLOGY; FEMALE; GEL; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; HUMAN; HUMAN TISSUE; LEG ULCER; MALE; MICROFLORA; MULTIVARIATE ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; POSTOPERATIVE INFECTION; PRIORITY JOURNAL; WOUND DRESSING; WOUND HEALING; WOUND INFECTION;

ADULT; AGED; AGED, 80 AND OVER; BACTERIA; CHRONIC DISEASE; COHORT STUDIES; CROSS-SECTIONAL STUDIES; DNA, BACTERIAL; FEMALE; HUMANS; MALE; METAGENOME; MIDDLE AGED; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SPECIMEN HANDLING; WOUNDS AND INJURIES;

EID: 78751502912     PISSN: 10671927     EISSN: 1524475X     Source Type: Journal    
DOI: 10.1111/j.1524-475X.2010.00628.x     Document Type: Article

References (20)

1. Mustoe TA, O'Shaughnessy K, Kloeters O., Chronic wound pathogenesis and current treatment strategies: a unifying hypothesis. Plast Reconstr Surg 2006; 117 (Suppl.): 35S-41S.
2. Fonder MA, Lazarus GS, Cowan DA, Aronson-Cook B, Kohli AR, Mamelak AJ., Treating the chronic wound: a practical approach to the care of nonhealing wounds and wound care dressings. J Am Acad Dermatol 2008; 158: 185-206.
3. Sen CK, Gordillo GM, Roy S, Kirsner R, Lambert L, Hunt TK, Gottrup F, Gurtner GC, Longaker MT., Human skin wounds: a major and snowballing threat to public health and the economy. Wound Repair Regen 2009; 17: 763-71.
4. Andersen A, Hill KE, Stephens P, Thomas DW, Jorgensen B, Krogfelt KA., Bacterial profiling using skin grafting, standard culture and molecular bacteriological methods. J Wound Care 2007; 16: 171-5.
5. Dowd SE, Sun Y, Secor PR, Rhoads DD, Wolcott BM, James GA, Wolcott RD., Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing. BMC Microbiol 2008; 8: 43.
6. Dowd SE, Wolcott RD, Sun Y, McKeehan T, Smith E, Rhoads D., Polymicrobial nature of chronic diabetic foot ulcer biofilm infections determined using bacterial tag encoded FLX amplicon pyrosequencing (bTEFAP). PLoS One 2008; 3: e3326.
7. Frank DN, Wysocki A, Specht-Glick DD, Rooney A, Feldman RA, St Amand AL, Pace NR, Trent JD., Microbial diversity in chronic open wounds. Wound Repair Regen 2009; 17: 163-72.
8. Frankel YM, Melendez JH, Wang NY, Price LB, Zenilman JM, Lazarus GS., Defining wound microbial flora: molecular microbiology opening new horizons. Arch Dermatol 2009; 145: 1193-5.
9. Price LB, Liu CM, Melendez JH, Frankel YM, Engelthaler D, Aziz M, Bowers J, Rattray R, Ravel J, Kingsley C, Keim PS, Lazarus GS, Zenilman JM., Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota. PLoS One 2009; 4: e6462.
10. Fazli M, Bjarnsholt T, Kirketerp-Màller K, Jàrgensen B, Andersen AS, Krogfelt KA, Givskov M, Tolker-Nielsen T., Nonrandom distribution of Pseudomonas aeruginosa and Staphylococcus aureus in chronic wounds. J Clin Microbiol 2009; 47: 4084-9.
11. Malic S, Hill KE, Hayes A, Percival SL, Thomas DW, Williams DW., Detection and identification of specific bacteria in wound biofilms using peptide nucleic acid fluorescent in situ hybridization (PNA FISH). Microbiology 2009; 155: 2603-11.
12. Wolcott RD, Gontcharova V, Sun Y, Dowd SE., Evaluation of the bacterial diversity among and within individual venous leg ulcers using bacterial tag-encoded FLX and titanium amplicon pyrosequencing and metagenomic approaches. BMC Microbiol 2009; 9: 226.
13. Cole JR, Wang Q, Cardenas E, Fish J, Chai B, Farris RJ, Kulam-Syed-Mohideen AS, McGarrell DM, Marsh T, Garrity GM, Tiedje JM., The ribosomal database project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res 2009; 37: D141-5.
14. R Development Core Team. R: A language and environment for statistical computing. R Development Core Team, 2009.
15. Goslee SC, Niering WA, Urban DL, Christensen NL., Influence of environment, history and vegetative interactions on stand dynamics in a connecticut forest. J Torrey Bot Soc 2005; 132: 471-82.
16. Murdoch D, Chow ED., Ellipse: Functions for drawing ellipses and ellipse-like confidence regions. Statistical package in R. 0.3-5, 2007.
17. Kindt R, Coe R., Tree diversity analysis. A manual and software for common statistical methods for ecological and biodiversity studies. Nairobi: World Agroforestry Centre (ICRAF), 2005.
18. Anderson MJ., A new method for non-parametric multivariate analysis of variance. Austral Ecol 2001; 26: 32-46.
19. Oksanen J, Kindt R, Legendre P, O'Hara B, Simpson GL, Solymos P, Stevens HH, Wagner H,. Vegan: community ecology package. 1.15-2, 2009.
20. Price LB, Liu CM, Johnson KE, Aziz M, Lau MK, Bowers J, Ravel J, Keim PS, Serwadda D, Wawer MJ, Gray RH., The effects of circumcision on the penis microbiome. PLoS One 2010; 5: e8422.