A whole-mechanical method to establish human embryonic stem cell line HN4 from discarded embryos

Cytotechnology

Volumn 62, Issue 6, 2010, Pages 509-518

  Li, Bin ^a,c   Xu, Lan ^b   Lu, Wei Ying ^a   Xu, Wen ^a   Wang, Mei Hong ^a   Yang, Ke ^b   Dong, Juan ^b   Ding, Xiao Yan ^b   Huang, Yuan Hua ^a  

^a HAINAN MEDICAL UNIVERSITY   (China)

^b INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^c FUDAN UNIVERSITY   (China)

Author keywords

Human stem cells; ICM mechanical isolation; Mixed feeder layer; Neural differentiation

Indexed keywords

ALKALINE PHOSPHATASE; OCTAMER TRANSCRIPTION FACTOR 4; STAGE SPECIFIC EMBRYO ANTIGEN 3; STAGE SPECIFIC EMBRYO ANTIGEN 4; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR REX 1; UNCLASSIFIED DRUG;

ARTICLE; CELL CULTURE; CELL ISOLATION; CELL STRAIN HN4; CONTROLLED STUDY; EMBRYO; EMBRYONIC STEM CELL; GENE EXPRESSION; GERM LAYER; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN EMBRYO; HUMAN TISSUE; IN VITRO STUDY; INNER CELL MASS; MECHANICAL STIMULATION; MOUSE; MOUSE EMBRYO; NERVE CELL DIFFERENTIATION; NONHUMAN; PROTEIN EXPRESSION; SKIN FIBROBLAST; TERATOMA;

EID: 78651480239     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-010-9311-z     Document Type: Article

References (34)

1. M Amit V Margulets H Segev K Shariki I Laevsky R Coleman, et al. 2003 Human feeder layers for human embryonic stem cells Biol Reprod 68 2150 2156 1:CAS:528:DC%2BD3sXks1Ggsb8%3D 10.1095/biolreprod.102.012583
2. B Balaban B Urman A Sertac C Alatas S Aksoy R Mercan 2000 Blastocyst quality affects the success of blastocyst-stage embryo transfer Fertil Steril 74 282 287 1:STN:280:DC%2BD3cvht1Wjsw%3D%3D 10.1016/S0015-0282(00)00645-2
3. MK Carpenter MS Inokuma J Denham T Mujtaba CP Chiu MS Rao 2001 Entrichment of neurons and neural precursors from human embryonic stem cells Exp Neurol 172 383 397 1:CAS:528:DC%2BD3MXosFGht78%3D 10.1006/exnr.2001.7832
4. MK Carpenter E Rosler MS Rao 2003 Characterization and differentiation of human embryonic stem cells Cloning Stem Cells 5 79 88 1:CAS:528: DC%2BD3sXis1eis7k%3D 10.1089/153623003321512193
5. L Cheng H Hammond Z Ye X Zhan G Dravid 2003 Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture Stem Cells 21 131 142 1:CAS:528:DC%2BD3sXis1Gmtrk%3D 10.1634/stemcells.21-2-131
6. A Choo J Padmanabhan A Chin WJ Fong SK Oh 2006 Immortalized feeders for the scale-up of human embryonic stem cells in feeder and feeder-free conditions J Biotechnol 122 130 141 1:CAS:528:DC%2BD28XhvVeis7w%3D 10.1016/j.jbiotec.2005. 09.008
7. T Dvash D Ben-Yosef R Eiges 2006 Human embryonic stem cells as a powerful tool for studying human embryogenesis Pediatr Res 60 111 117 10.1203/01.pdr.0000228349.24676.17
8. M Familari L Selwood 2006 The potential for derivation of embryonic stem cells in vertebrates Mol Reprod Dev 73 123 131 1:CAS:528:DC%2BD2MXht12ntb3M 10.1002/mrd.20376
9. N Findikli S Kahraman O Akcin S Sertyel Z Candan 2005 Establishment and characterization of new human embryonic stem cell lines Reprod Biomed Online 10 617 627 10.1016/S1472-6483(10)61669-0
10. N Heins A LindahI U Karlsson M Rehnström G Caisander K Emanuelsson, et al. 2006 Clonal derivation and characterization of human embryonic stem cell lines J Biotechnol 122 511 520 1:CAS:528:DC%2BD28XjtFOms7g%3D 10.1016/j.jbiotec.2005.10.010
11. S Hong UJ Kang O Isacson KS Kim 2008 Neural precursors derived from human embryonic stem cells mainstain long-term proliferation without losing the potential to differentiate into all three neural lineages, including dopaminergic neurons J Neurochem 104 316 324 1:CAS:528:DC%2BD1cXhtlSqsr8%3D
12. O Hovatta M Mikkola K Gertow AM Strömberg J Inzunza J Hreinsson, et al. 2003 A culture system using foreskin fibroblasts as a feeder cells allow production of human embryonic stem cells Hum Reprod 18 1404 1409 10.1093/humrep/deg290
13. J Itskovitz-Eldor M Schuldiner D Karsenti A Eden O Yanuka M Amit, et al. 2000 Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germ layers Mol Med 6 88 95 1:STN:280: DC%2BD3cvgt1WksA%3D%3D
14. HS Keirstead G Nistor G Bernal M Totoiu F Cloutier K Sharp, et al. 2005 Human embryonic stem cell-derived oligodendrocyte progenitor cell transplants remyelinate and restore locomotion after spinal cord injury J Neurosci 25 4694 4705 1:CAS:528:DC%2BD2MXksFalt70%3D 10.1523/JNEUROSCI.0311-05.2005
15. PH Lerou GQ Daley 2005 Therapeutic potential of embryonic stem cells Blood Rev 19 321 331 10.1016/j.blre.2005.01.005
16. PH Lerou A Yabuuchi H Huo JD Miller LF Boyer TM Schlaeger, et al. 2008 Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos Nat Protoc 3 923 933 1:CAS:528:DC%2BD1cXltlSgsb0%3D 10.1038/nprot.2008.60
17. B Li QP Peng WY Lu W Xu YX Jin YH Huang 2008 Growth state of human embryonic stem cells on mixed feeder layers with mouse embryonic fibroblasts and human foreskin fibroblasts at different ratios CRTER 12 424 428 1:CAS:528:DC%2BD1MXktlCqsb0%3D
18. W Ma T Tavakoli E Derby Y Serebryakova MS Rao MP Mattson 2008 Cell-extracellular matrix interactions regulate neural differentiation of human embryonic stem cells BMC Dev Biol 8 90 10.1186/1471-213X-8-90
19. A Mandal S Tipnis R Pal G Ravindran B Bose A Patki, et al. 2006 Characterization and in vitro differentiation potential of a new human embryonic stem cell line, ReliCellhES1 Differentiation 74 81 90 1:CAS:528: DC%2BD28XjsVCmsb0%3D 10.1111/j.1432-0436.2006.00051.x
20. C Mummery 2004 Stem cell research: immortality or healthy old age? Eur J Endocrinol 151 Suppl. 3 U7 U12 1:CAS:528:DC%2BD2cXhtFSht7vN 10.1530/eje.0. 151U007
21. JH Park SJ Kim EJ Oh SY Moon SI Roh CG Kim, et al. 2003 Establishment and maintenance of human embryonic stem cells on STO, a permanently growing cell line Biol Reprod 69 2007 2014 1:CAS:528:DC%2BD3sXpsVCnsb8%3D 10.1095/biolreprod.103.017467
22. K Qian H Chen SM Zhang GJ Zhu 2006 Human fibroblast cell supporting undifferentiated human embryonic stem cells proliferation in vitro Acta Med Univ Sci Technol Huazhong 35 462 464 1:CAS:528:DC%2BD2sXhtlWhtL3E
23. BE Reubinoff MF Pera CY Fong A Trounson A Bongso 2000 Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro Nat Biotechnol 18 399 404 1:CAS:528:DC%2BD3cXis1Gmt74%3D 10.1038/74447
24. M Richards CY Fong WK Chan PC Wong A Bongso 2002 Human feeders support prolonged undifferentiated growth of human inner cells masses and embryonic stem cell lines Nat Biotechnol 20 933 936 1:CAS:528:DC%2BD38Xms1ahsL8%3D 10.1038/nbt726
25. M Richards S Tan CY Fong A Biswas WK Chan A Bongso 2003 Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells Stem Cells 21 546 556 1:CAS:528:DC%2BD3sXnvFeisrY%3D 10.1634/stemcells.21-5-546
26. Robertson EJ (1987) Embryo-derived stem cell lines. In: Robertson EJ (ed) Teratocarcinomas and embryonic stem cells. IRL Press, Oxford, pp 71-112
27. S Shin M Mitalipova S Noggle D Tibbitts A Venable P Rao, et al. 2006 Long-term proliferation of human embryonic stem cell-derived neuroepithelial cells using defined adherent culture conditions Stem Cells 24 125 138 10.1634/stemcells.2004-0150
28. D Srivastava KN Ivey 2006 Potential of stem cell-based therapies for heart disease Nature 441 1097 1099 1:CAS:528:DC%2BD28Xmtlahs7w%3D 10.1038/nature04961
29. P Stojkovic M Lako R Stewart S Przyborski L Armstrong J Evans, et al. 2005 An autogeneic feeder cell system that efficiently supports growth of undifferentiated human embryonic stem cells Stem Cells 23 306 314 1:CAS:528:DC%2BD2MXjtFyjtLc%3D 10.1634/stemcells.2004-0137
30. S Ström J Inzunza KH Grinnemo K Holmberg E Matilainen AM Strömberg, et al. 2007 Mechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines Human Reprod 22 3051 3058 10.1093/humrep/dem335
31. JA Thomson J Itskovitz-Eldor SS Shapiro MA Waknitz JJ Swiergiel VS Marshall, et al. 1998 Embryonic stem cell lines derived from human blastocysts Science 282 1145 1147 1:CAS:528:DyaK1cXntleisLg%3D 10.1126/science.282.5391.1145
32. SV Urban J Kiss V Vas J Kovacs F Uher 2006 Stem cell therapy for diabetes mellitus: progress, prospects and challenges Orv Hetil 147 791 797
33. A Van de Stolpe S van den Brink M van Rooijen D Ward-van Oostwaard W van Inzen I Slaper-Cortenbach, et al. 2005 Human embryonic stem cells: towards therapies for cardiac disease. Derivation of a Dutch human embryonic stem cell line Reprod Biomed Online 11 476 485 10.1016/S1472-6483(10)61144-3
34. SC Zhang M Wernig ID Duncan O Brustle JA Thomson 2001 In vitro differentiation of transplantable neural precursors from human embryonic stem cells Nat Biotechnol 19 1129 1133 1:CAS:528:DC%2BD3MXptV2rtrc%3D 10.1038/nbt1201-1129