Application of comet assay in the study of DNA damage and recovery in rohu (Labeo rohita) fingerlings after an exposure to phorate, an organophosphate pesticide

Ecotoxicology

Volumn 20, Issue 1, 2011, Pages 283-292

  Mohanty, G ^d   Mohanty, J ^a   Nayak, A K ^b   Mohanty, S ^a   Dutta, S K ^c  

^a CENTRAL INSTITUTE OF FRESHWATER AQUACULTURE   (India)

^b GOVERNMENT SCIENCE COLLEGE   (India)

^c NORTH ORISSA UNIVERSITY   (India)

^d NONE

Author keywords

Comet assay; Genotoxicity; Gills; Labeo rohita; Liver; Pesticide

Indexed keywords

PHORATE;

CELL ORGANELLE; CONCENTRATION (COMPOSITION); CYPRINID; DETECTION METHOD; DNA; ECOTOXICOLOGY; ELECTROKINESIS; GENOTOXICITY; ORGANOPHOSPHATE; PESTICIDE; POLLUTION EFFECT; POLLUTION EXPOSURE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; COMET ASSAY; CONTROLLED STUDY; DNA REPAIR; DNA STRAND BREAKAGE; ECOTOXICOLOGY; ENVIRONMENTAL MONITORING; FINGERLING; FISH; FRESHWATER SPECIES; GENOTOXICITY; GILL; IN VIVO STUDY; LABEO ROHITA; LIVER CELL CULTURE; LONG TERM EXPOSURE; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN DNA BINDING; TISSUE SPECIFICITY; TOXICITY TESTING; WATER POLLUTANT;

ANIMALS; BLOOD CELLS; COMET ASSAY; CYPRINIDAE; DNA DAMAGE; DNA REPAIR; LIVER; PESTICIDES; PHORATE; WATER POLLUTANTS, CHEMICAL;

LABEO ROHITA; METAZOA; PISCES;

EID: 78651469294     PISSN: 09639292     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10646-010-0580-2     Document Type: Article

References (44)

1. BS Banu K Danadevi MF Rahman YR Ahuja J Kaiser 2001 Genotoxic effect of monocrotophos to sentinel species using comet assay Food Chem Toxicol 39 361 366 10.1016/S0278-6915(00)00141-1
2. K Belpaeme K Cooreman M Kirsch-Volders 1998 Development and validation of the in vivo alkaline comet assay for detecting genomic damage in marine flatfish Mutat Res 415 167 184 1:CAS:528:DyaK1cXks1Ontrg%3D
3. J Blasiak P Jaloszynski A Trzeciak K Szyfter 1999 In vitro studies on the genotoxicity of the organophosphorus insecticide malathion and its two analogues Mutat Res 445 275 283 1:CAS:528:DyaK1MXns1Ghsro%3D
4. Cajaraville MP, Hauser L, Carvalho G, Hylland K, Olabarrieta I, Lawrence AJ, Lowe D, Goksoyr A (2003) Genetic damage and the molecular/cellular response to pollution. In: Lawrence AJ, Hemingway KL (eds) Effects of pollution on fish: molecular effects and population responses. Blackwell Publishing Company, Oxford, pp 14-62
5. T Cavas S Ergene-Gozukara 2003 Evaluation of the genotoxic potential of lambda cyhalothrin using nuclear and nucleolar biomarkers on fish cells Mutat Res 534 93 99 1:CAS:528:DC%2BD38Xpslaks7Y%3D
6. T Cavas S Ergene-Gozukara 2005 Induction of micronuclei and nuclear abnormalities in Oreochromis niloticus following exposure to petroleum refinery and chromium processing plant effluents Aquat Toxicol 74 264 271 1:CAS:528:DC%2BD2MXns1Wjtrw%3D 10.1016/j.aquatox.2005.06.001
7. P Chakrabarty V Banarjee 1988 Effect of organophosphorus pesticides on the peripheral hemogram of the fish Channa punctatus Environ Ecol 6 390 394 1:CAS:528:DyaL1MXntVOquw%3D%3D
8. EWK Ching WHL Siu PKS Lam L Xu Y Zhang BJ Richardson RSS Wu 2001 DNA adduct formation and DNA strand breaks in green-lied mussels (Perna viridis) exposed to benzo[a]pyrene: dose- and time-dependent relationships Mar Pollut Bull 42 603 610 1:CAS:528:DC%2BD3MXlslGhsLc%3D 10.1016/S0025-326X(00)00209-5
9. AR Collins 2004 The comet assay for DNA damage and repair: principles, applications, and limitations Mol Biotechnol 26 249 261 1:CAS:528: DC%2BD2cXivF2gu7c%3D 10.1385/MB:26:3:249
10. P Das G John 1999 Induction of sister chromatid exchanges and chromosome aberrations in vivo in Etroplus suratensis (Bloch) following exposure to organophosphorus pesticides Toxicol Lett 104 111 116 1:CAS:528: DyaK1cXnsFajt78%3D 10.1016/S0378-4274(98)00355-5
11. DW Fairbairn L OliveJ' KL O'Neill 1995 The comet assay: a comprehensive review Mutat Res 339 37 59 1:CAS:528:DyaK2MXktlChsro%3D
12. S Feng Z Kong X Wang P Peng YE Zeng 2005 Assessing the genotoxicity of imidacloprid and RH-5849 in human peripheral blood lymphocytes in vitro with comet assay and cytogenetic tests Ecotoxicol Environ Saf 61 239 246 1:CAS:528:DC%2BD2MXjvFelt7g%3D 10.1016/j.ecoenv.2004.10.005
13. M Frankenberg-Schwager 1989 Review of repair kinetics for DNA damage induced in eukaryotic cells in vitro by ionizing radiation Radiother Oncol 14 307 320 1:CAS:528:DyaL1MXktlGrsro%3D 10.1016/0167-8140(89)90143-6
14. S Haider M Inbaraj 1986 Relative toxicity of technical material and commercial formulation of malathion and endosulfan to a freshwater fish, Channa punctatus (Bloch). Ecotoxicol Environ Saf 11 347 351 1:CAS:528: DyaL28XkslCjtL8%3D 10.1016/0147-6513(86)90107-7
15. JS Harvey JM Parry 1997 The detection of genotoxin induced DNA adducts in the common mussel Mytilus edulis Mutagenesis 12 153 158 1:CAS:528: DyaK2sXjsV2qsLg%3D 10.1093/mutage/12.3.153
16. M Hayashi T Ueda K Uyeno K Wada N Kinae K Saotome N Tanaka A Takai YF Sasaki N Asano T Sofuni Y Ojima 1998 Development of genotoxicity assay systems that use aquatic organisms Mutat Res 399 125 133 1:CAS:528:DyaK1cXjsVensLk%3D
17. MC Henderson SK Krueger LK Siddens JF Stevens DE Williams 2004 S-Oxygenation of the thioether organophosphate insecticides phorate and disulfoton by human lung flavin-containing monooxygenase 2 Biochem Pharmacol 68 959 967 1:CAS:528:DC%2BD2cXmt1KmtLY%3D 10.1016/j.bcp.2004.05.051
18. E Hodgson PE Lcvi 1996 Pesticides: an important but underused model for the environmental health sciences Environ Health Perspect 106 115 125
19. SE Hook RF Lee 2004 Interactive effects of UV, benzo[a]pyrene, and cadmium on DNA damage and repair in embryos of the grass shrimp Paleomonetes pugio Mar Environ Res 58 735 739 1:CAS:528:DC%2BD2cXkslShsrk%3D 10.1016/j.marenvres.2004.03.087
20. B Jyothi G Narayan 1999 Certain pesticide-induced carbohydrate metabolic disorders in the serum of freshwater fish Clarias batrachus (Linn.) Food Chem Toxicol 37 417 421 1:CAS:528:DyaK1MXkt12nu7o%3D 10.1016/S0278-6915(99)00020-4
21. B Jyothi G Narayan 2000 Pesticide induced alterations of non-protein nitrogenous constituents in the serum of freshwater catfish, Clarias batrachus (Linn.) Indian J Exp Biol 38 233 235
22. B Jyothi G Narayan 2001 Effect of pesticides carbaryl and phorate on serum cholesterol levels in fish, Clarias batrachus (Linn.) J Environ Biol 22 233 235 1:CAS:528:DC%2BD3MXns1Gqtrs%3D
23. MZ Khan FCP Law 2005 Adverse effects of pesticides and related chemicals on enzyme and hormone systems of fish, amphibians and reptiles: a review Proc Pakistan Acad Sci 42 315 323
24. Kidd H, James DR (1991) The agrochemical handbook, 3rd edn. Royal Society of Chemistry Information Services, Cambridge, pp 3-11
25. Kligerman D (1982) Fishes as biological detectors of the effects of genotoxic agents. In: Heddle J (ed) New horizons in genetic toxicology, mutagenicity. Academic, New York, pp 435-456
26. RF Lee S Steinert 2003 Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic (marine and freshwater) animals Mutat Res 544 43 64 1:CAS:528:DC%2BD3sXlvVWnsL4%3D 10.1016/S1383-5742(03)00017-6
27. NG Lemos AL Dias AT Silva-Souza MS Mantovani 2005 Evaluation of environmental waters using the comet assay in Tilapia rendalli Environ Toxicol Pharmacol 19 197 201 1:CAS:528:DC%2BD2cXhtVKrtLnJ 10.1016/j.etap.2004.03.011
28. Loeb SL, Spacie A (1994) Biological monitoring for aquatic systems. Lewis Publishers, Boca Raton
29. GK Manna G Banerjee S Gupta 1985 Micronucleus test in the peripheral erythrocytes of the exotic fish Nucleus 23 176 179
30. CL Mitchelmore JK Chipman 1998 DNA strand breakage in aquatic organisms and the potential value of the comet assay in the environmental monitoring Mutat Res 399 135 147 1:CAS:528:DyaK1cXjsVensLY%3D
31. D Nacci S Nelson W Nelson E Jackim 1992 Application of the DNA alkaline unwinding assay to detect DNA strand breaks in marine bivalves Mar Environ Res 33 83 100 1:CAS:528:DyaK3sXhslCrtQ%3D%3D 10.1016/0141-1136(92)90134-8
32. SB Nadin LM Vargas-Roig DR Ciocca 2001 A silver staining method for single-cell gel assay J Histochem Cytochem 49 1183 1186 1:CAS:528: DC%2BD3MXmsFyltLk%3D
33. S Pandey NS Nagpure R Kumar S Sharma SK Srivastava MS Verma 2006 Genotoxicity evaluation of acute doses of endosulfan to freshwater teleost Channa punctatus (Bloch) by alkaline single cell gel electrophoresis Ecotoxicol Environ Saf 65 56 61 1:CAS:528:DC%2BD28Xot1ahtLc%3D 10.1016/j.ecoenv.2005.06.007
34. R Pandrangi M Petras S Ralph M Vrzoc 1995 Alkaline single cell gel (comet) assay and genotoxicity monitoring using bullheads and carp Environ Mol Mutagen 26 345 356 1:CAS:528:DyaK28XjsFyntQ%3D%3D 10.1002/em.2850260411
35. AM Pruski DR Dixon 2003 Toxic vents and DNA damage: first evidence from a naturally contaminated deep-sea environment Aquat Toxicol 6 1 13 10.1016/S0166-445X(03)00010-9
36. LR Shugart C Theodorakis 1996 Genetic ecotoxicology: the genotypic diversity approach Comp Biochem Physiol C 113 273 276
37. Silva J, Heuser V, Andrade V (2003) Biomonitoramento ambiental. In: Silva J, Erdtmann B, Henriques JAP (eds) Genetica toxicologica. Alcance, Porto Alegre
38. WHL Siu CL Hung HL Wong BJ Richardson PK Lam 2003 Exposure and time dependent DNA strand breakage in hepatopancreas of green-lied mussels (Perna viridis) exposed to Aroclor 1254, and mixtures of B[a]P and Aroclor 1254 Mar Pollut Bull 46 1285 1293 1:CAS:528:DC%2BD3sXnvVWqsrg%3D 10.1016/S0025-326X(03) 00234-0
39. WHL Siu J Cao RW Jack RSS Wu BJ Richardson L Xu PK Lam 2004 Application of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lied mussel (Perna viridis) Aquat Toxicol 66 381 392 1:CAS:528:DC%2BD2cXhtV2mtr4%3D 10.1016/j.aquatox.2003.10.006
40. Smith GJ (1993) Toxicology and pesticide use in relation to wildlife: organophosphorus and carbamate compounds. C. K. Smoley, Boca Raton, pp 5-7
41. G Speit A Hartmann 1995 The contribution of excision repair to the DNA effects seen in the alkaline single cell gel test (comet assay) Mutagenesis 10 555 559 1:CAS:528:DyaK2MXpslaqsb8%3D 10.1093/mutage/10.6.555
42. SA Steinert 1996 Contribution of apoptosis to observed DNA damage in mussel cells in pollutant responses in marine organisms (PRIMO 8) Mar Environ Res 42 253 259 1:CAS:528:DyaK28XjvF2mt7g%3D 10.1016/0141-1136(95)00056-9
43. M Sumathi K Kalaiselvi M Palanivel P Rajaguru 2001 Genotoxicity of textile dye effluent on fish (Cyprinus carpio) measured using the comet assay Bull Environ Contam Toxicol 66 407 414 1:CAS:528:DC%2BD3MXhtV2ksbY%3D 10.1007/s00128-001-0020-3
44. G Wirzinger L Weltje J Gercken H Sordyl 2007 Genotoxic damage in field-collected three-spined sticklebacks (Gasterosteus aculeatus L.): a suitable biomonitoring tool? Mutat Res 628 19 30 1:CAS:528:DC%2BD2sXitFeqtLs%3D