Electrospray ionization-mass spectrometry conformational analysis of isolated domains of an intrinsically disordered protein

Biotechnology Journal

Volumn 6, Issue 1, 2011, Pages 96-100

  Testa, Lorenzo ^a   Brocca, Stefania ^a   Šamalikova, Maria ^a   Santambrogio, Carlo ^a   Alberghina, Lilia ^a   Grandori, Rita ^a  

^a UNIVERSITY OF MILANO BICOCCA   (Italy)

Author keywords

Charge state distributions; Cyclin dependent protein kinase inhibitors; Nano electrospray; Partially folded states; Sic1

Indexed keywords

CHARGE STATE DISTRIBUTION; CYCLIN-DEPENDENT PROTEIN KINASE INHIBITORS; ELECTROSPRAYS; FOLDED STATE; SIC1;

IONIZATION; MASS SPECTROMETRY; PROTEINS; STRUCTURAL ANALYSIS; YEAST;

ELECTROSPRAY IONIZATION;

CELL PROTEIN; CYCLIN DEPENDENT KINASE INHIBITOR; INTRINSICALLY DISORDERED PROTEIN; PROTEIN SIC1; UNCLASSIFIED DRUG;

ARTICLE; ELECTROSPRAY MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN FOLDING; SACCHAROMYCES CEREVISIAE;

CYCLIN-DEPENDENT KINASE INHIBITOR PROTEINS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

SACCHAROMYCES CEREVISIAE;

EID: 78651327259     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201000253     Document Type: Article

References (20)

1. Fink, A. L., Natively unfolded proteins. Curr. Opin. Struct. Biol. 2005, 15, 35-41.
2. Uversky, V. N., Oldfield, C. J., Dunker, A. K., Showing your ID: Intrinsic disorder as an ID for recognition, regulation and cell signaling. J. Mol. Recognit. 2005, 18, 343-384.
3. Eliezer, D., Biophysical characterization of intrinsically disordered proteins. Curr. Opin. Struct. Biol. 2009, 19, 23-30.
4. Konermann, L., Rosell, F. I., Mauk, A. G., Douglas, D. J., Acid-induced denaturation of myoglobin studied by time-resolved electrospray ionization mass spectrometry. Biochemistry 1997, 36, 6448-6454.
5. Kaltashov, I. A., Abzalimov, R. R., Do ionic charges in ESI MS provide useful information on macromolecular structure? J. Am. Soc. Mass Spectrom. 2008, 19, 1239-1246.
6. Schwob, E., Bohm, T., Mendenhall, M. D., Nasmyth, K., The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae. Cell 1994, 79, 233-244.
7. Brocca, S., Samalikova, M., Uversky, V. N., Lotti, M. et al., Order propensity of an intrinsically disordered protein, the cyclin-dependent-kinase inhibitor Sic1. Proteins 2009, 76, 731-746.
8. Brocca, S., Testa, L., Samalikova, M., Grandori, R. et al., Defining structural domains of an intrinsically disordered protein: Sic1, the cyclin-dependent kinase inhibitor of Saccharomyces cerevisiae. Mol. Biotechnol. 2010, DOI:10.1007/s12033-010-9309-y.
9. Uversky, V. N., Fink, A. L., Amino acid determinants of alpha-synuclein aggregation: Putting together pieces of the puzzle. FEBS Lett. 2002, 522, 9-13.
10. Hodge, A., Mendenhall, M., The cyclin-dependent kinase inhibitory domain of the yeast Sic1 protein is contained within the C-terminal 70 amino acids. Mol. Gen. Genet. 1999, 262, 55-64.
11. Barberis, M., De Gioia, L., Ruzzene, M., Sarno, S. et al., The yeast cyclin-dependent kinase inhibitor Sic1 and mammalian p27Kip1 are functional homologues with a structurally conserved inhibitory domain. Biochem. J. 2005, 387, 639-647.
12. Galea, C. A., Wang, Y., Sivakolundu, S. G., Kriwacki, R. W., Regulation of cell division by intrinsically unstructured proteins: Intrinsic flexibility, modularity, and signaling conduits. Biochemistry 2008, 47, 7598-7609.
13. Borysik, A. J., Radford, S. E., Ashcroft, A. E., Co-populated conformational ensembles of beta2-microglobulin uncovered quantitatively by electrospray ionization mass spectrometry. J. Biol. Chem. 2004, 279, 27069-27077.
14. Peng, K., Vucetic, S., Radivojac, P., Brown, C. J. et al., Optimizing long intrinsic disorder predictors with protein evolutionary information. J. Bioinform. Comput. Biol. 2005, 3, 35-60.
15. Marsh, J. A., Forman-Kay, J. D., Sequence determinants of compaction in intrinsically disordered proteins. Biophys. J. 2010, 98, 2383-2390.
16. Dobo, A., Kaltashov, I. A., Detection of multiple protein conformational ensembles in solution via deconvolution of charge-state distributions in ESI MS. Anal. Chem. 2001, 73, 4763-4773.
17. Kuprowski, M. C., Konermann, L., Signal response of coexisting protein conformers in electrospray mass spectrometry. Anal. Chem. 2007, 79, 2499-2506.
18. Heck, A. J., Van Den Heuvel, R. H., Investigation of intact protein complexes by mass spectrometry. Mass Spectrom. Rev. 2004, 23, 368-389.
19. Espinoza-Fonseca, L. M., Leucine-rich hydrophobic clusters promote folding of the N-terminus of the intrinsically disordered transactivation domain of p53. FEBS Lett. 2009, 583, 556-560.
20. Mittag, T., Marsh, J., Grishaev, A., Orlicky, S. et al., Structure/function implications in a dynamic complex of the intrinsically disordered Sic1 with the Cdc4 subunit of an SCF ubiquitin ligase. Structure 2010, 18, 494-506.