Isolation of granulocytes: Which transcriptome do we analyse - Neutrophils or eosinophils?

Folia Biologica

Volumn 56, Issue 6, 2010, Pages 252-255

  Stejskal, S ^a   Koutná, Irena ^a   Rucka Z ^a  

^a MASARYK UNIVERSITY   (Czech Republic)

Author keywords

Cell isolation; Eosinophils; Expression activity; Gene expression; Gradient centrifugation; Granulocytes; Magnetic separation; Neutrophils; Total RNA

Indexed keywords

DNA; FLUORESCEIN ISOTHIOCYANATE; RNA; RNA POLYMERASE II; TRANSCRIPTION FACTOR; TRANSCRIPTOME;

ARTICLE; CENTRIFUGATION; DNA REPAIR; DNA STRAND BREAKAGE; EOSINOPHIL; GENE EXPRESSION; GENETIC TRANSCRIPTION; GRANULOCYTE; HUMAN; HUMAN CELL; NEUTROPHIL; RNA SYNTHESIS;

EID: 78651274291     PISSN: 00155500     EISSN: 25337602     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Cowland, J. B., Borregaard, N. (1999) Isolation of neutrophil precursors from bone marrow for biochemical and transcriptional analysis. J. Immunol. Methods 232, 191-200.
2. Domachowski, J. B., Rosenberg, H. F. (2000) The role of eosinophils and their ribonucleases in respiratory viral infections. Respir. Med. 94, 1012-014.
3. Freitas, M., Porto, G., Lima, J. L., Fernandes, E. (2008) Isolation and activation of human neutrophils in vitro. The importance of the anticoagulant used during blood collection. Clin. Biochem. 41, 570-575.
4. Hämäläinen, M. M., Eskola, J. U., Hellman, J., Pulkki, K. (1999) Major interference from leukocytes in reverse transcription-PCR identified as neurotoxin ribonuclease from eosinophils: detection of residual chronic myelogenous leukemia from cell lysates by use of an eosinophil-depleted cell preparation. Clin. Chem. 45, 465-471.
5. Rozell, M. D., Erger, R. A., Casale, T. B. (1996) Isolation technique alters eosinophil migration response to IL-8. J. Immunol. Methods 197, 97-107.
6. Sabroe, I., Jones, E. C., Usher, L. R., Whyte, M. K. B., Dower, S. K. (2002) Toll-like receptor (TLR)2 and TLR4 in human peripheral blood granulocytes: a critical role for monocytes in leukocyte lipopolysaccharide responses. J. Immunol. 168, 4701-10.
7. Sabroe, I., Prince, L. R., Dower, S. K., Walmsley, S. R., Chilvers, E. R., Whyte, M. K. B. (2004) What can we learn from highly purified neutrophils? Biochem. Soc. Trans. 32, 468-469.
8. Salati, S., Bianchi, E., Zini, R., Tenedini, E., Quaglino, D., Manfredini, R., Ferrari, S. (2007) Eosinophils, but not neutrophils, exhibit an efficient DNA repair machinery and high nucleolar activity. Haematologica 92, 1311-1318.
9. Samoszuk, M. (2006) Isolation of human eosinophils from peripheral blood using hypotonic lysis and centrifugation. Am. J. Hematol. 81, 552-553.
10. Schefzyk, M., Bruder, M., Schmiedl, A., Stephan, M., Kapp, A., Wedi, B., Raap, U. (2009) Eosinophil granulocytes: functional differences of a new isolation kit compared to the isolation with anti-CD 16-conjugated MicroBeads. Exp. Dermatol. 18, 653-655.
11. Sedgwick, J. B., Shikama, Y., Nagata, M., Brener, K., Busse, W. W. (1996) Effect of isolation protocol on eosinophil function: Percoll gradients versus immunomagnetic beads. J. Immunol. Methods 198, 15-24.
12. Stejskal, S., Koutna, I., Matula, P., Rucka, Z., Danek, O., Maska, M., Kozubek, M. (2010) The role of chromatin condensation during granulopoiesis in the regulation of gene cluster expression. Epigenetics 5, 759-767.
13. Weil, G. J., Chused, T. M. (1981) Eosinophil autofluorescence and its use in isolation and analysis of human eosinophils using flow microfluorometry. Blood 57, 1099-1104.
14. Zahler, S., Kowalski, C., Brosig, A., Kupatt, C., Becker, B. F., Gerlach, E. (1997) The function of neutrophils isolated by a magnetic antibody cell separation technique is not altered in comparison to a density gradient centrifugation method. J. Immunol. Methods 200, 173-179.