Quantification of prognostic immune cell markers in colorectal cancer using whole slide imaging tumor maps

Analytical and Quantitative Cytology and Histology

Volumn 32, Issue 6, 2010, Pages 333-340

  Halama, Niels ^a   Zoernig, Inka ^a   Spille, Anna ^a   Michel, Sara ^a   Kloor, Matthias ^a   Grauling Halama, Silke ^a   Westphal, Kathi ^a   Schirmacher, Peter ^a   Jäger, Dirk ^a   Grabe, Niels ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Colorectal cancer; Immune cells; Prognosis; Virtual microscopy

Indexed keywords

CD3 ANTIGEN; CD45RO ANTIGEN; CD8 ANTIGEN; GRANZYME B;

ARTICLE; CANCER SURVIVAL; CELL DENSITY; CELL HETEROGENEITY; CLINICAL ARTICLE; COLORECTAL CANCER; CONTROLLED STUDY; FEMALE; HUMAN; HUMAN TISSUE; IMAGING SYSTEM; IMMUNOCOMPETENT CELL; MALE; MICROSCOPY; PRIORITY JOURNAL; PROGNOSIS; QUANTITATIVE ANALYSIS; TISSUE MICROARRAY; WHOLE SLIDE IMAGING;

CELL COUNT; COLORECTAL NEOPLASMS; HUMANS; MICROARRAY ANALYSIS; PROGNOSIS; TUMOR MARKERS, BIOLOGICAL;

EID: 78650827530     PISSN: 08846812     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Bhargava R, Lal P, Chen B: Feasibility of using tissue microarrays for the assessment of HER-2 gene amplification by fluorescence in situ hybridization in breast carcinoma. Diagn Mol Pathol 2004;13:213-216
2. Donati V, Faviana P, Dell'omodarme M, Prati MC, Camacci T, De Ieso K, Giannini R, Lucchi M, Mussi A, Pingitore R, Basolo F, Fontanini G: Applications of tissue microarray technology in immunohistochemistry: A study on c-kit expression in small cell lung cancer. Hum Pathol 2004;35:1347-1352
3. Kyndi M, Sørensen FB, Knudsen H, Overgaard M, Nielsen HM, Andersen J, Overgaard J: Tissue microarrays compared with whole sections and biochemical analyses: A subgroup analysis of DBCG 82 b&c. Acta Oncol 2008;47:591-599
4. Merseburger AS, Kuczyk MA, Serth J, Bokemeyer C, Young DY, Sun L, Connelly RR, McLeod DG, Mostofi FK, Srivastava SK, Stenzl A, Moul JW, Sesterhenn IA: Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues. Oncol Rep 2003;10:223-228
5. Ruiz C, Seibt S, Al Kuraya K, Siraj AK, Mirlacher M, Schraml P, Maurer R, Spichtin H, Torhorst J, Popovska S, Simon R, Sauter G: Tissue microarrays for comparing molecular features with proliferation activity in breast cancer. Int J Cancer 2006;118:2190-2194
6. Nocito A, Bubendorf L, Tinner EM, Süess K, Wagner U, Forster T, Kononen J, Fijan A, Bruderer J, Schmid U, Ackermann D, Maurer R, Alund G, Knönagel H, Rist M, Anabitarte M, Hering F, Hardmeier T, Schoenenberger AJ, Flury R, Jäger P, Fehr JL, Schraml P, Moch H, Mihatsch MJ, Gasser T, Sauter G: Microarrays of bladder cancer tissue are highly representative of proliferation index and histological grade. J Pathol 2001;194:349-357
7. Goethals L, Perneel C, Debucquoy A, De Schutter H, Borghys D, Ectors N, Geboes K, McBride WH, Haustermans KM: A new approach to the validation of tissue microarrays. J Pathol 2006;208:607-614
8. Kononen J, Bubendorf L, Kallioniemi A, Bärlund M, Schraml P, Leighton S, Torhorst J, Mihatsch MJ, Sauter G, Kallioniemi OP: Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med 1998;4:844-847
9. Conway C, Dobson L, O'Grady A, Kay E, Costello S, O'Shea D: Virtual microscopy as an enabler of automated/quantitative assessment of protein expression in TMAs. Histochem Cell Biol 2008;130:447-463
10. Flaberg E, Sabelstrom P, Strandh C, Szekely L: Extended field laser confocal microscopy (EFLCM): Combining automated gigapixel image capture with in silico virtual microscopy. BMC Med Imaging 2008;8:13
11. Grabe N: Virtual microscopy in systems pathology. Pathologe 2008;29:259-263
12. Jemal A, Siegel R, Ward E, Murray T, Xu J, Smigal C, Thun MJ: Cancer statistics, 2006. CA Cancer J Clin 2006;56:106-130
13. Galon J, Costes A, Sanchez-Cabo F, Kirilovsky A, Mlecnik B, Lagorce-Pagès C, Tosolini M, Camus M, Berger A, Wind P, Zinzindohoué F, Bruneval P, Cugnenc PH, Trajanoski Z, Fridman WH, Pagès F: Type, density, and location of immune cells within human colorectal tumors predict clinical outcome. Science 2006;313:1960-1964
14. Menon AG, Janssen-van Rhijn CM, Morreau H, Putter H, Tollenaar RA, van de Velde CJ, Fleuren GJ, Kuppen PJ: Immune system and prognosis in colorectal cancer: A detailed immunohistochemical analysis. Lab Invest 2004;84:493-501
15. Prall F, Dührkop T, Weirich V, Ostwald C, Lenz P, Nizze H, Barten M: Prognostic role of CD8+ tumor-infiltrating lymphocytes in stage III colorectal cancer with and without microsatellite instability. Hum Pathol 2004;35:808-816
16. Naito Y, Saito K, Shiiba K, Ohuchi A, Saigenji K, Nagura H, Ohtani H: CD8+ T cells infiltrated within cancer cell nests as a prognostic factor in human colorectal cancer. Cancer Res 1998;58:3491-3494 (Pubitemid 28376536)
17. Ohtani H: Focus on TILs: Prognostic significance of tumor infiltrating lymphocytes in human colorectal cancer. Cancer Immun 2007;7:4
18. Halama N, Michel S, Kloor M, Zoernig I, Pommerencke T, von Knebel Doeberitz M, Schirmacher P, Weitz J, Grabe N, Jäger D: The localization and density of immune cells in primary tumors of human metastatic colorectal cancer shows an association with response to chemotherapy. Cancer Immun 2009;9:1
19. Pagès F, Berger A, Camus M, Sanchez-Cabo F, Costes A, Molidor R, Mlecnik B, Kirilovsky A, Nilsson M, Damotte D, Meatchi T, Bruneval P, Cugnenc PH, Trajanoski Z, Fridman WH, Galon J: Effector memory T cells, early metastasis, and survival in colorectal cancer. N Engl J Med 2005;353:2654-2666
20. Nagorsen D, Scheibenbogen C, Letsch A, Germer CT, Buhr HJ, Hegewisch-Becker S, Rivoltini L, Thiel E, Keilholz U: T cell responses against tumor associated antigens and prognosis in colorectal cancer patients. J Transl Med 2005;3:3
21. Chiba T, Ohtani H, Mizoi T, Naito Y, Sato E, Nagura H, Ohuchi A, Ohuchi K, Shiiba K, Kurokawa Y, Satomi S: Intraepithelial CD8+ T-cell-count becomes a prognostic factor after a longer follow-up period in human colorectal carcinoma: Possible association with suppression of micrometastasis. Br J Cancer 2004;91:1711-1717
22. Bubendorf L, Nocito A, Moch H, Sauter G: Tissue microarray (TMA) technology: Miniaturized pathology archives for high-throughput in situ studies. J Pathol 2001;195:72-79
23. Halama N, Zoernig I, Spille A, Westphal K, Schirmacher P, Jaeger D, Grabe N: Estimation of immune cell densities in immune cell conglomerates: An approach for high-throughput quantification. PLoS One 2009;4:e7847