N-Glycosylation efficiency is determined by the distance to the C-terminus and the amino acid preceding an Asn-Ser-Thr sequon

Protein Science

Volumn 20, Issue 1, 2011, Pages 179-186

  Bañó Polo, Manuel ^a   Baldin, Francesca ^a   Tamborero, Silvia ^a   Marti Renom, Marc A ^b   Mingarro, Ismael ^a  

^a UNIVERSITY OF VALENCIA   (Spain)

^b CENTRO DE INVESTIGACIÓN PRÍNCIPE FELIPE   (Spain)

Author keywords

C terminus tagging; Glycosylation efficiency; Membrane protein topology; Oligosaccharyltransferase acceptor site; Sequon

Indexed keywords

ASPARAGINE; GLYCOPROTEIN; MEMBRANE PROTEIN; PROTEIN TAG; SERINE; THREONINE;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; GLYCOSYLATION; IN VITRO STUDY; MICROSOME; PRIORITY JOURNAL; PROTEIN ANALYSIS;

AMINO ACID MOTIFS; ANIMALS; CELL-FREE SYSTEM; CONSENSUS SEQUENCE; DOGS; ESCHERICHIA COLI PROTEINS; GLYCOSYLATION; MEMBRANE PROTEINS; MICROSOMES; PROTEIN MODIFICATION, TRANSLATIONAL; RABBITS; RECOMBINANT PROTEINS; SERINE ENDOPEPTIDASES;

EUKARYOTA;

EID: 78650799822     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.551     Document Type: Article

References (30)

1. Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, Shindyalov IN, Bourne PE (2000) The Protein Data Bank. Nucleic Acids Res 28: 235-242.
2. von Heijne G (2006) Membrane-protein topology. Nat Rev Mol Cell Biol 7: 909-918.
3. White SH (2004) The progress of membrane protein structure determination. Protein Sci 13: 1948-1949.
4. White SH (2009) Biophysical dissection of membrane proteins. Nature 459: 344-346.
5. von Heijne G (1992) Membrane protein structure prediction-hydrophobicity analysis and the positive-Inside rule. J Mol Biol 225:487-494.
6. Alder NN, Johnson AE (2004) Cotranslational membrane protein biogenesis at the endoplasmic reticulum. J Biol Chem 279: 22787-22790.
7. Welply JK, Shenbagamurthi P, Lennarz WJ, Naider F (1983) Substrate recognition by oligosaccharyltransferases. Studies on glycosylation of modified asn-x-ser/thr tripeptides. J Biol Chem 258: 11856-11863.
8. Cheung JC, Reithmeier RA (2007) Scanning N-glycosylation mutagenesis of membrane proteins. Methods 41: 451-459.
9. von Heijne G (1989) Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues. Nature 341: 456-458.
10. Nilsson I, von Heijne G (1993) Determination of the distance between the oligosaccharyltransferase active site and the endoplasmic reticulum membrane. J Biol Chem 268: 5798-5801.
11. Martinez-Gil L, Sanchez-Navarro JA, Cruz A, Pallas V, Perez-Gil J, Mingarro I (2009) Plant virus cell-to-cell movement is not dependent on the transmembrane disposition of its movement protein. J Virol 83: 5535-5543.
12. Orzaez M, Salgado J, Gimenez-Giner A, Perez-Paya E, Mingarro I (2004) Influence of proline residues in transmembrane helix packing. J Mol Biol 335:631-640.
13. Gavel Y, von Heijne G (1990) Sequence differences between glycosylated and non-glycosylated Asn-X-Thr/Ser acceptor sites-implications for protein engineering. Protein Eng 3: 433-442.
14. Ben-Dor S, Esterman N, Rubin E, Sharon N (2004) Biases and complex patterns in the residues flanking protein N-glycosylation sites. Glycobiology 14: 95-101.
15. Vilar M, Sauri A, Monne M, Marcos JF, von Heijne G, Perez-Paya E, Mingarro I (2002) Insertion and topology of a plant viral movement protein in the endoplasmic reticulum membrane. J Biol Chem 277: 23447-23452.
16. Nilsson I, von Heijne G (2000) Glycosylation efficiency of Asn-Xaa-Thr sequons depends both on the distance from the C terminus and on the presence of a downstream transmembrane segment. J Biol Chem 275: 17338-17343.
17. Hamilton SR, Yao SY, Ingram JC, Hadden DA, Ritzel MW, Gallagher MP, Henderson PJ, Cass CE, Young JD, Baldwin SA (2001) Subcellular distribution and membrane topology of the mammalian concentrative Na+-nucleoside cotransporter rCNT1. J Biol Chem 276: 27981-27988.
18. The UniProt Consortium, T.U. (2010). The universal protein resource (UniProt) in 2010. Nucleic Acids Res 38: D142-148.
19. Mellquist JL, Kasturi L, Spitalnik SL, Shakin-Eshleman SH (1998) The amino acid following an asn-X-Ser/Thr sequon is an important determinant of N-linked core glycosylation efficiency. Biochemistry 37: 6833-6837.
20. Shakin-Eshleman SH, Spitalnik SL, Kasturi L (1996) The amino acid at the X position of an Asn-X-ser sequon is an important determinant of N-linked coreglycosylation efficiency. J Biol Chem 271: 6363-6366.
21. Kasturi L, Chen H, Shakin-Eshleman SH (1997) Regulation of N-linked core glycosylation: use of a site directed mutagenesis approach to identify Asn-Xaa-Ser/Thr sequons that are poor oligosaccharide acceptors. Biochem J 323: 415-419.
22. Petrescu AJ, Milac AL, Petrescu SM, Dwek RA, Wormald MR (2004) Statistical analysis of the protein environment of N-glycosylation sites: implications for occupancy, structure, and folding. Glycobiology 14: 103-114.
23. Jones J, Krag SS, Betenbaugh MJ (2005) Controlling N-linked glycan site occupancy. Biochim Biophys Acta 1726:121-137.
24. Kowarik M, Young NM, Numao S, Schulz BL, Hug I, Callewaert N, Mills DC,Watson DC, Hernandez M, Kelly JF, Wacker M, Aebi M. (2006) Definition of the bacterial N-glycosylation site consensus sequence. EMBO J 25: 1957-1966.
25. Landolt-Marticorena C, Reithmeier RAF (1994) Asparagine-linked oligosaccharides are localized to single extracytosolic segments in multi-span membrane glycoproteins. Biochem J 302: 253-260.
26. Hessa T, Kim H, Bihlmaier K, Lundin C, Boekel J, Andersson H, Nilsson I, White SH, von Heijne G (2005) Recognition of transmembrane helices by the endoplasmic reticulum translocon. Nature 433: 377-381.
27. Martinez-Gil L, Sauri A, Vilar M, Pallas V, Mingarro I (2007) Membrane insertion and topology of the p7B movement protein of Melon Necrotic Spot Virus (MNSV). Virology 367: 348-357.
28. Sauri A, Tamborero S, Martinez-Gil L, Johnson AE, Mingarro I (2009) Viral membrane protein topology is dictated by multiple determinants in its sequence. J Mol Biol 387: 113-128.
29. Martinez-Gil L, Johnson AE, Mingarro I (2010) Membrane insertion and biogenesis of the Turnip crinkle virus p9 movement protein. J Virol 84: 5520-5527.
30. Li W, Godzik A (2006) Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences. Bioinformatics 22: 1658-1659.