Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR

Virology Journal

Volumn 7, Issue , 2010, Pages

  Zhao, Kai ^a   Han, Fangting ^b   Zou, Yong ^a   Zhu, Lianlong ^a   Li, Chunhua ^a   Xu, Yan ^b   Zhang, Chunling ^a   Tan, Furong ^a   Wang, Jinbin ^a   Tao, Shiru ^a   He, Xizhong ^a   Zhou, Zongqing ^a   Tang, Xueming ^a  

^a INSTITUTE OF EDIBLE FUNGI   (China)

^b SHANGHAI NORMAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; PRIMER DNA;

ARTICLE; CIRCOVIRUS; DIARRHEA; DISEASE TRANSMISSION; GASTROENTERITIS; GENE EXPRESSION; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; ROTAVIRUS; SWINE; ANIMAL; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; POLYMERASE CHAIN REACTION; POSTWEANING MULTISYSTEMIC WASTING SYNDROME; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VIROLOGY;

PORCINE CIRCOVIRUS 1; PORCINE CIRCOVIRUS 2; ROTAVIRUS; SUIDAE; SUS;

ANIMALS; CIRCOVIRUS; DNA PRIMERS; POLYMERASE CHAIN REACTION; PORCINE POSTWEANING MULTISYSTEMIC WASTING SYNDROME; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SWINE;

EID: 78650664755     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/1743-422X-7-374     Document Type: Article

References (16)

1. Porcine circoviruses: a review. GM Allan JA Ellis, J Vet Diagn Invest 2000 12 3 14 10690769
2. Porcine circovirus-2 and concurrent infections in the field. J Ellis E Clark D Haines K West S Krakowka S Kennedy GM Allan, Vet Microbiol 2004 98 159 163 10.1016/j.vetmic.2003.10.008 14741128
3. Postweaning multisystemic wasting syndrome (PMWS) in pigs: a review. J Segales M Domingo, Vet Q 2002 24 109 124 12400999
4. Pathological findings associated with naturally acquired porcine circovirus type 2 associated disease. J Segales C Rosell M Domingo, Vet Microbiol 2004 98 137 149 10.1016/j.vetmic.2003.10.006 14741126
5. Association of myocarditis with high viral load of porcine circovirus type 2 in several tissues in cases of fetal death and high mortality in piglets: a case study. IM Brunborg CM Jonassen T Moldal B Bratberg B Lium F Koenen J Schönheit, J Vet Diagn Invest 2007 19 368 375 17609345
6. Isolation and characterization of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. GM Allan F McNeilly BM Meehan S Kennedy DP Mackie JA Ellis EG Clark E Espuna N Saubi P Riera, Vet Microbiol 1999 66 115 123 10.1016/S0378-1135(99)00004-8 10227473
7. Research progress of porcine circovirus: a review. DM Wu YC Cao YJ Bi, Chin J Prev Vet Med 2004 26 1 76 78
8. Development of reverse transcription-PCR assays specific for detection of equine encephalitis viruses. B Linssen RM Kinney P Aguilar KL Russell DM Watts OR Kaaden M Pfeffer, J Clin Microbiol 2000 38 1527 1535 10747138
9. Porcine circoviruses: a review. GM Allan JA Ellis, J Vet Diagn Invest 2000 12 3 14 10690769
10. A study investigating epidemiological risk factors for porcine circovirus type II in Ontario. TS Cottrell RM Friendship CE Dewey G Josephson G Allan I Walker, Pig J 1999 44 10 17
11. Development and application of a competitive enzyme-linked immunosorbent assay for the detection of serum antibodies to porcine circovirus type 2. IW Walker CA Konoby VA Jewhurst I McNair F McNeilly BM Meehan TS Cottrell JA Ellis GM Allan, J Vet Diagn Invest 2000 12 400 405 11021425
12. Antibody detection of weaning multisystemic wasting syndrome. HW L GC Zhang FQ Wu, Chin J Vet Sci Tech 2000 23 591 592
13. Cellular adaptive immune response against porcine circovirus type 2 in subclinically infected pigs. S Esther B Carole G Heidi S Artur MC Kenneth, BMC Vet Res 2009 5 45 10.1186/1746-6148-5-45 20028550
14. Quantitation of porcine circovirus type 2 isolated from serum/plasma and tissue samples of healthy pigs and pigs with postweaning multisystemic wasting syndrome using a TaqMan-based real-time PCR. IM Brunborg T Moldal CM Jonassen, J Virol Methods 2004 122 2 171 178 10.1016/j.jviromet.2004.08.014 15542141
15. Real-time PCR for quantitation of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in naturally-infected and challenged pigs. WB Chung WH Chan HC Chaung Y Lien CC Wu YL Huang, J Virol Methods 2005 124 11 19 10.1016/j.jviromet.2004.10.003 15664045
16. Detection of PCV2 DNA by SYBR Green I-based quantitative PCR. ZZ Yang Mudasser Habib JB Shuai WH Fang, J Zhejiang Univ Sci B 2007 8 3 162 169 10.1631/jzus.2007.B0162 17323427