Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: Case of a nonglycosylated mutant of miraculin, a taste-modifying protein

Biotechnology Letters

Volumn 33, Issue 1, 2011, Pages 103-107

  Ito, Keisuke ^a,b   Sugawara, Taishi ^a   Koizumi, Ayako ^a   Nakajima, Ken Ichiro ^a   Shimizu Ibuka, Akiko ^a   Shiroishi, Mitsunori ^c   Asada, Hidetsugu ^c   Yurugi Kobayashi, Takami ^c   Shimamura, Tatsuro ^c   Asakura, Tomiko ^a   Misaka, Takumi ^a   Iwata, So ^c,d,e   Kobayashi, Takuya ^c,d   Abe, Keiko ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b UNIVERSITY OF SHIZUOKA   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^d KYOTO UNIVERSITY   (Japan)

^e IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Cysteine; Disulfide bond; Mutagenesis; Protein secretion; Screening method; Yeast expression system

Indexed keywords

AMINO ACIDS; COVALENT BONDS; MUTAGENESIS; SULFUR COMPOUNDS; YEAST;

CYSTEINE; DISULFIDE BONDS; PROTEIN SECRETION; SCREENING METHODS; YEAST EXPRESSION;

PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 78650515514     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0399-1     Document Type: Article

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