Comparison of real-time PCR and conventional PCR for detection of leishmania (leishmania) infantum infection: A mini-review

Journal of Venomous Animals and Toxins Including Tropical Diseases

Volumn 16, Issue 4, 2010, Pages 537-542

  Paiva Cavalcanti, M ^a   Regis da Silva, C G ^a   Gomes, Y M ^a  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

Author keywords

Diagnosis; Molecular diagnosis; Visceral leishmaniasis

Indexed keywords

PRIMER DNA;

ARTICLE; COST EFFECTIVENESS ANALYSIS; DNA EXTRACTION; DNA SEQUENCE; DOG; GENE AMPLIFICATION; HISTOPATHOLOGY; HUMAN; INTERMETHOD COMPARISON; LEISHMANIA INFANTUM; MICROORGANISM DETECTION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SEROLOGY; VISCERAL LEISHMANIASIS;

LEISHMANIA INFANTUM;

EID: 78650317190     PISSN: 16789199     EISSN: 16789199     Source Type: Journal    
DOI: 10.1590/S1678-91992010000400004     Document Type: Article

References (31)

1. Chappuis F, Sundar S, Hailu A, Ghalib H, Rijal S, Peeling RW, et al. Visceral leishmaniasis: what are the needs for diagnosis, treatment and control? Nat Rev Microbiol. 2007;5(11):873-882.
2. Gomes YM, Paiva-Cavalcanti M, Lira RA, Abath FG, Alves LC. Diagnosis of canine visceral leishmaniasis: biotechnological advances. Vet J. 2008;175(1):45-52.
3. Schallig HD, Oskam L. Molecular biological applications in the diagnosis and control of leishmaniasis and parasite identification. Trop Med Int Health. 2002;7(8): 641-651.
4. Nicolas L, Prina E, Lang T, Milon G. Real-time PCR for detection and quantitation of Leishmania in mouse tissues. J Clin Microbiol. 2002;40(5):1666-1669.
5. Zarlenga DS, Higgins J. PCR as a diagnostic and quantitative technique in veterinary parasitology. Vet Parasitol. 2001;101(3-4):215-230.
6. Bastien P, Procop GW, Reischl U. Quantitative real-time PCR is not more sensitive than conventional PCR. J Clin Microbiol. 2008;46(6):1897-1900.
7. Yang S, Rothman RE. PCR-based diagnostics for infectious diseases: uses, limitations and future applications in acute-care settings. Lancet Infect Dis. 2004;4(6):337-348.
8. Bahk YY, Kim SA, Kim JS, Euh HJ, Bai GH, Cho SN, et al. Antigens secreted from Mycobacterium tuberculosis: identification by proteomics approach and test for diagnostic marker. Proteomics. 2004;4(11):3299-3307.
9. Higuchi R, Dollinger G, Walsh PS, Griffith R. Simultaneous amplification and detection of specific DNA sequences. Biotechnology. 1992;10(4):413-417.
10. Mortarino M, Franceschi A, Mancianti F, Bazzocchi C, Genchi C, Bandi C. Quantitative PCR in the diagnosis of Leishmania. Parassitologia. 2004;46(1-2):163-167.
11. Kubista M, Andrade JM, Bengtsson M, Forootan A, Jonák J, et al. The real time polymerase chain reaction. Mol Aspects Med. 2006;27(2-3):95-125.
12. Applied Biosystems. Real Time PCR Systems. Absolute Quantification Getting Started Guide. Atlanta, 2005. Available from: http://www.appliedbiosystems.com. Access in: Aug. 16, 2006.
13. Too HP. Real time PCR quantification of GFR-2 alternatively spliced isoforms in murine brain and peripheral tissues. Mol Brain Res. 2003;114(2):146-153.
14. Paiva-Cavalcanti M, Brito MEF, Souza WV, Gomes YM, Abath FGC. The development of a real-time PCR assay for the quantification of Leishmania infantum DNA in canine blood. Vet J. 2009;182(2):356-358.
15. Ferreira ID, Rosário VE, Cravo PV. Real-time quantitative PCR with SYBR Green I detection for estimating copy numbers of nine drug resistance candidate genes in Plasmodium falciparum. Malar J. 2006;5:1.
16. Robledo G, González CI, Morillo C, Martín J, González A. Association study of PTPN22 C1858T polymorphism in Trypanosoma cruzi infection. Tissue Antigens. 2007;69(3):261-264.
17. Jonhson VJ, Yucesoy YB, Luster MI. Genotyping of single nucleotide polymorphisms in cytokine genes using real-time PCR allelic discrimination technology. Cytokine. 2004;27(6):135-141.
18. Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polimerase-catalyzed reaction. Methods Enzymol. 1987;155:335-350.
19. Fichoux YL, Quaranta JF, Aufeuvre JP, Lelievre A, Marty P, Suffia I, Rousseau D, et al. Occurrence of Leishmania infantum parasitemia in asymptomatic blood donors living in an area of endemicity in southern France. J Clin Microbiol. 1999;37(6):1953-1957.
20. Ravel S, Cuny G, Reynes J, Veas F. A highly sensitive and rapid procedure for direct PCR detection of Leishmania infantum within human peripheral blood mononuclear cells. Acta Trop. 1995;59(3):187-196.
21. Soares MJV, Noraes JRE, Roselino AMF. Polymerase chain reaction in detecting Leishmania sp. symptomatic and asymptomatic seropositive dogs. J Venom Anim Toxins incl Trop Dis. 2005;11(4):532-539.
22. Lachaud L, Chabbert E, Dubessay P, Dereure J, Lamothe J, Dedet JP, et al. Value of two PCR methods for the diagnosis of canine visceral leishmaniasis and the detection of asymptomatic carriers. Parasitology. 2002;125(Pt 3):197-207.
23. Gomes AHS, Ferreira IMR, Lima MLSR, Cunha EA, Garcia AS, Araújo MFL, et al. PCR identification of Leishmania in diagnosis and control of canine leishmaniasis. Vet Parasitol. 2007;144(3-4):234-241.
24. Cerino DA, Veloso J, Perles TF, Zanzarini PD, Lonardoni MVC, Silveira TGV. American cutaneous leishmaniasis in dogs from an endemic urban area in Cianorte municipality, Paraná State, Brazil. J Venom Anim Toxins incl Trop Dis. 2010;16(2): 241-252.
25. Rodgers MR, Popper SJ, Wirth DF. Amplification of Kinetoplast DNA as a tool in the detection and diagnosis of Leishmania. Exp Parasitol. 1990;71(3):267-275.
26. Nunes CM, Dias AKK, Gottardi FP, Paula HB, Azevedo MAA, Lima VMF. Avaliação da reação em cadeia da polimerase para diagnóstico da leishmaniose visceral em sangue de cães. Rev Bras Parasitol Vet. 2007;16(1):5-9.
27. Manna L, Vitale F, Reale S, Caracappa S, Pavone LM, Morte RD, et al. Comparison of different tisuue sampling for PCR-based diagnosis and follow-up of canine visceral leishmaniosis. Vet Parasitol. 2004;125(3-4):251-262.
28. Mary C, Faraut F, Lascombe L, Dumon H. Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity. J Clin Microbiol. 2004;42(11): 5249-5255.
29. Francino O, Altet L, Sanchez-Robert E, Rodriguez A, Solano-Gallego L, Alberola J, et al. Advantages of real-time PCR assay for diagnosis and monitoring of canine leishmaniosis. Vet Parasitol. 2006;137(3-4):214-21.
30. Schulz A, Mellenthin K, Schonian G, Fleischer B, Drosten C. Detection, differentiation, and quantitation of pathogenic Leishmania organisms by a fluorescence resonance energy transfer-based real-time PCR assay. J Clin Microbiol. 2003;41(4):1529-1535.
31. Paiva-Cavalcanti M, Lorena VMB, Gomes YM. Avanços biotecnológicos para o diagnóstico das doenças infecciosas e parasitárias. Rev Patol Trop. 2008;37(1):1-14.