Purification and characterisation of a novel protease from Cordyceps sinensis and determination of the cleavage site motifs using oriented peptide library mixtures

Food Chemistry

Volumn 126, Issue 1, 2011, Pages 46-53

  Bi, Bo ^a   Wang, Xinyu ^a   Wu, Hezhen ^a   Wei, Qun ^a  

^a BEIJING NORMAL UNIVERSITY   (China)

Author keywords

Cleavage site motifs; Cordyceps sinensis; Oriented peptide library; Protease; Purification

Indexed keywords

ANION EXCHANGE CHROMATOGRAPHY; BIOCHEMICAL PROPERTIES; CHARACTERISATION; CLEAVAGE SITES; COMMERCIALISATION; CORDYCEPS SINENSIS; DE NOVO SEQUENCING; INDUSTRIALISATION; MALDI TOF MS; NATURAL PROTEINS; ORIENTED PEPTIDE LIBRARY; PEPTIDE LIBRARY; PHASE CHROMATOGRAPHY; PROTEASE; SDS-PAGE; SUBSTRATE SPECIFICITY; SYNTHETIC PEPTIDE;

AMINO ACIDS; HYDROLASES; INDUSTRIAL ECONOMICS; MIXTURES; PEPTIDES; PURIFICATION;

CHROMATOGRAPHY;

DETERGENT; LYSINE; OXIDIZING AGENT; PEPTIDE LIBRARY; PROLINE; PROTEINASE; PROTEINASE INHIBITOR; REDUCING AGENT; SYNTHETIC PEPTIDE;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; ENZYME SPECIFICITY; OPHIOCORDYCEPS; PH; PROTEIN CLEAVAGE; PROTEIN MOTIF; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; TEMPERATURE SENSITIVITY;

CORDYCEPS SINENSIS; FUNGI;

EID: 78650182710     PISSN: 03088146     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodchem.2010.10.057     Document Type: Article

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