Influence of N-acetylcysteine administration on pulmonary O2 uptake kinetics and exercise tolerance in humans

Respiratory Physiology and Neurobiology

Volumn 175, Issue 1, 2011, Pages 121-129

  Bailey, Stephen J ^a   Winyard, Paul G ^a   Blackwell, Jamie R ^a   Vanhatalo, Anni ^a   Lansley, Katherine E ^a   DiMenna, Fred J ^a   Wilkerson, Daryl P ^a   Campbell, Iain T ^b   Jones, Andrew M ^a  

^a UNIVERSITY OF EXETER   (United Kingdom)

^b WYTHENSHAWE HOSPITAL   (United Kingdom)

Author keywords

Antioxidant; Fatigue; Oxidative metabolism; Plasma nitrite; Thiols

Indexed keywords

ACETYLCYSTEINE; LACTIC ACID; NITRITE; PLACEBO; POTASSIUM; SODIUM CHLORIDE; THIOL GROUP;

ADULT; ARTICLE; CLINICAL ARTICLE; EXERCISE INTENSITY; EXERCISE TEST; EXERCISE TOLERANCE; HUMAN; KINETICS; LACTATE BLOOD LEVEL; MALE; OXYGEN CONSUMPTION; PRIORITY JOURNAL;

ACETYLCYSTEINE; ADULT; ANALYSIS OF VARIANCE; BIOMECHANICS; EXERCISE TEST; EXERCISE TOLERANCE; FREE RADICAL SCAVENGERS; HUMANS; MALE; NITRIC OXIDE; OXYGEN CONSUMPTION; PLASMA VOLUME; PULMONARY GAS EXCHANGE; PULMONARY VENTILATION; TIME FACTORS; YOUNG ADULT;

EID: 78650169522     PISSN: 15699048     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.resp.2010.10.002     Document Type: Article

References (75)

1. Andrews N.P., Prasad A., Quyyumi A.A. N-acetylcysteine improves coronary and peripheral vascular function. J. Am. Coll. Cardiol. 2001, 37:117-123.
2. Aruoma O.I., Halliwell B., Hoey B.M., Butler J. The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Radic. Biol. Med. 1989, 6:593-597.
3. 2 cost of low-intensity exercise and enhances tolerance to high-intensity exercise in humans. J. Appl. Physiol. 2009, 107:1144-1155.
4. 2 uptake kinetics and severe-intensity exercise tolerance. J. Appl. Physiol. 2009, 107:1743-1756.
5. 2 uptake and muscle deoxygenation kinetics in humans. J. Appl. Physiol. 2009, 106:1875-1887.
6. Bailey S.J., Winyard P.G., Vanhatalo A., Blackwell J.R., Dimenna F.J., Wilkerson D.P., Tarr J., Benjamin N., Jones A.M. Dietary nitrate supplementation enhances muscle contractile efficiency during knee extensor exercise in humans. J. Appl. Physiol. 2010, 109:135-148.
7. Balon T.W., Nadler J.L. Nitric oxide release is present from incubated skeletal muscle preparations. J. Appl. Physiol. 1994, 77:2519-2521.
8. 2+ cycling in skeletal muscle. J. Muscle Res. Cell Motil. 2007, 28:259-274.
9. Benrahmoune M., Thérond P., Abedinzadeh Z. The reaction of superoxide radical with N-acetylcysteine. Free Radic. Biol. Med. 2000, 29:775-782.
10. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
11. Burnley M., Jones A.M. Oxygen uptake kinetics as a determinant of sports performance. Eur. J. Sports Sci. 2007, 7:63-79.
12. Cotgreave I.A. N-acetylcysteine: pharmacological considerations and experimental and clinical applications. Adv. Pharmacol. 1997, 38:205-227.
13. Deneke S.M. Thiol-based antioxidants. Curr. Top. Cell. Regul. 2000, 36:151-180.
14. Ellman G.L. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 1959, 82:70-77.
15. Ferreira L.F., Reid M.B. Muscle-derived ROS and thiol regulation in muscle fatigue. J. Appl. Physiol. 2008, 104:853-860.
16. 2 uptake kinetics in humans: implications for metabolic control. J. Appl. Physiol. 1996, 80:988-998.
17. Herspring K.F., Ferreira L.F., Copp S.W., Snyder B.S., Poole D.C., Musch T.I. Effects of antioxidants on contracting spinotrapezius muscle microvascular oxygenation and blood flow in aged rats. J. Appl. Physiol. 2008, 105:1889-1896.
18. Hogg N., Singh R.J., Kalyanaraman B. The role of glutathione in the transport and catabolism of nitric oxide. FEBS Lett. 1996, 382:223-228.
19. Hu M.L., Louie S., Cross C.E., Motchnik P., Halliwell B. Antioxidant protection against hypochlorous acid in human plasma. J. Lab. Clin. Med. 1993, 121:257-262.
20. Huang K.T., Huang Z., Kim-Shapiro B.D. Nitric oxide red blood cell membrane permeability at high and low oxygen tension. Nitric Oxide 2007, 16:209-216.
21. Huang A., Xiao H., Samii J.M., Vita J.A., Keaney J.F. Contrasting effects of thiol-modulating agents on endothelial NO bioactivity. Am. J. Physiol. Cell Physiol. 2001, 281:719-725.
22. Jaimes E.A., Sweeney C., Raij L. Effects of the reactive oxygen species hydrogen peroxide and hypochlorite on endothelial nitric oxide production. Hypertension 2001, 38:877-883.
23. Jenkins R.R., Krause K., Schofield L.S. Influence of exercise on clearance of oxidant stress products and loosely bound iron. Med. Sci. Sports Exerc. 1993, 25:213-217.
24. Jones A.M., Burnley M. Oxygen uptake kinetics: an underappreciated determinant of exercise performance. Int. J. Sports Physiol. Perform. 2009, 4:524-532.
25. Jones A.M., Wilkerson D.P., Koppo K., Wilmshurst S., Campbell I.T. Inhibition of nitric oxide synthase by l-NAME speeds phase II pulmonary V̇O2 kinetics in the transition to moderate-intensity exercise in man. J. Physiol. 2003, 552:265-272.
26. 2 uptake kinetics during heavy-intensity cycle exercise. J. Appl. Physiol. 2004, 96:1033-1038.
27. Juel C. The effect of beta 2-adrenoceptor activation on ion-shifts and fatigue in mouse soleus muscles stimulated in vitro. Acta Physiol. Scand. 1988, 134:209-216.
28. Kleinbongard P., Dejam A., Lauer T., Rassaf T., Schindler A., Picker O., Scheeren T., Gödecke A., Schrader J., Schulz R., Heusch G., Schaub G.A., Bryan N.S., Feelisch M., Kelm M. Plasma nitrite reflects constitutive nitric oxide synthase activity in mammals. Free Radic. Biol. Med. 2003, 35:790-796.
29. Kobzik L., Reid M.B., Bredt D.S., Stamler J.S. Nitric oxide in skeletal muscle. Nature 1994, 372:546-548.
30. Koechlin C., Couillard A., Simar D., Cristol J.P., Bellet H., Hayot M., Prefaut C. Does oxidative stress alter quadriceps endurance in chronic obstructive pulmonary disease?. Am. J. Respir. Crit. Care Med. 2004, 169:1022-1027.
31. Koga S., Shiojiri T., Shibasaki M., Kondo N., Fukuba Y., Barstow T.J. Kinetics of oxygen uptake during supine and upright heavy exercise. J. Appl. Physiol. 1999, 87:253-260.
32. Krogh A., Lindhard J. The changes in respiration at the transition from work to rest. J. Physiol. 1920, 53:431-439.
33. 2 uptake kinetics during moderate- and high-intensity sub-maximal knee-extensor exercise in humans. J. Physiol. 2009, 587:1843-1856.
34. Krustrup P., Soderlund K., Mohr M., Bangsbo J. The slow component of oxygen uptake during intense, sub-maximal exercise in man is associated with additional fibre recruitment. Pflugers Arch. 2004, 447:855-866.
35. Lamarra N., Whipp B.J., Ward S.A., Wasserman K. Effect of interbreath fluctuations on characterising exercise gas exchange kinetics. J. Appl. Physiol. 1987, 62:2003-2012.
36. Lauer T., Kleinbongard P., Kelm M. Indexes of NO bioavailability in human blood. News Physiol. Sci. 2002, 17:251-255.
37. Lauer T., Preik M., Rassaf T., Strauer B.E., Deussen A., Feelisch M., Kelm M. Plasma nitrite rather than nitrate reflects regional endothelial nitric oxide synthase activity but lacks intrinsic vasodilator action. Proc. Natl. Acad. Sci. U.S.A. 2001, 98:12814-12819.
38. Maccarrone M., Ullrich V. Redox regulation in disease and ageing. Cell Death Differ. 2004, 11:949-951.
39. Matuszczak Y., Farid M., Jones J., Lansdowne S., Smith M.A., Taylor A.A., Reid M.B. Effects of N-acetylcysteine on glutathione oxidation and fatigue during handgrip exercise. Muscle Nerve 2005, 32:633-638.
40. McArdle F., Pattwell D.M., Vasilaki A., McArdle A., Jackson M.J. Intracellular generation of reactive oxygen species by contracting skeletal muscle cells. Free Radic. Biol. Med. 2005, 39:651-657.
41. +-pump activity and delays fatigue during prolonged exercise in humans. J. Physiol. 2006, 576:279-288.
42. Medved I., Brown M.J., Bjorksten A.R., Leppik J.A., Sostaric S., McKenna M.J. N-acetylcysteine infusion alters blood redox status but not time to fatigue during intense exercise in humans. J. Appl. Physiol. 2003, 94:1572-1582.
43. Medved I., Brown M.J., Bjorksten A.R., McKenna M.J. Effects of intravenous N-acetylcysteine infusion on time to fatigue and potassium regulation during prolonged cycling exercise. J. Appl. Physiol. 2004, 96:211-217.
44. Medved I., Brown M.J., Bjorksten A.R., Murphy K.T., Petersen A.C., Sostaric S., Gong X., McKenna M.J. N-acetylcysteine enhances muscle cysteine and glutathione availability and attenuates fatigue during prolonged exercise in endurance-trained individuals. J. Appl. Physiol. 2004, 97:1477-1485.
45. Merry T.L., Wadley G.D., Stathis C.G., Garnham A.P., Rattigan S., Hargreaves M., McConell G.K. N-Acetylcysteine infusion does not affect glucose disposal during prolonged moderate-intensity exercise in humans. J. Physiol. 2010, 588:1623-1634.
46. 2+ sensitivity in fatiguing mouse skeletal muscle at 37°C. J. Physiol. 2005, 564:189-199.
47. Pechanova O., Kunes J., Dobesova Z., Vrankova S., Zicha J. Contribution of neuronal nitric oxide (NO) synthase to N-acetylcysteine-induced increase of NO synthase activity in the brain of normotensive and hypertensive rats. J. Physiol. Pharmacol. 2009, 60:21-25.
48. Poole D.C., Barstow T.J., McDonough P., Jones A.M. Control of oxygen uptake during exercise. Med. Sci. Sports Exerc. 2008, 40:462-474.
49. Poole D.C., Ward S.A., Gardner G.W., Whipp B.J. Metabolic and respiratory profile of the upper limit for prolonged exercise in man. Ergonomics 1988, 31:1265-1279.
50. Powers S.K., Jackson M.J. Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiol. Rev. 2008, 88:1243-1276.
51. Rassaf T., Lauer T., Heiss C., Balzer J., Mangold S., Leyendecker T., Rottler J., Drexhage C., Meyer C., Kelm M. Nitric oxide synthase-derived plasma nitrite predicts exercise capacity. Br. J. Sports Med. 2007, 41:669-673.
52. Reardon T.F., Allen D.G. Time to fatigue is increased in mouse muscle at 37°C; the role of iron and reactive oxygen species. J. Physiol. 2009, 587:4705-4716.
53. Reid M.B. Free radicals and muscle fatigue: of ROS, canaries, and the IOC. Free Radic. Biol. Med. 2008, 44:169-179.
54. Reid M.B. Invited review: redox modulation of skeletal muscle contraction: what we know and what we don't. J. Appl. Physiol. 2001, 90:724-731.
55. Reid M.B., Haack K.E., Franchek K.M., Valberg P.A., Kobzik L., West M.S. Reactive oxygen in skeletal muscle. I. Intracellular oxidant kinetics and fatigue in vitro. J. Appl. Physiol. 1992, 73:1797-1804.
56. Reid M.B., Khawli F.A., Moody M.R. Reactive oxygen in skeletal muscle. III. Contractility of unfatigued muscle. J. Appl. Physiol. 1993, 75:1081-1087.
57. Reid M.B., Shoji T., Moody M.R., Entman M.L. Reactive oxygen in skeletal muscle. II. Extracellular release of free radicals. J. Appl. Physiol. 1992, 73:1805-1809.
58. Reid M.B., Stokić D.S., Koch S.M., Khawli F.A., Leis A.A. N-acetylcysteine inhibits muscle fatigue in humans. J. Clin. Invest. 1994, 94:2468-2474.
59. Riddles P.W., Blakeley R.L., Zerner B. Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid) - a reexamination. Anal. Biochem. 1979, 94:75-81.
60. 2 uptake between the on- and off-transients of moderate- and high-intensity exercise in humans. J. Physiol. 2002, 541:991-1002.
61. Sen C.K. Nutritional biochemistry of cellular glutathione. J. Nutr. Biochem. 1997, 8:660-672.
62. Shindoh C., DiMarco A., Thomas A., Manubay P., Supinski G. Effect of N-acetylcysteine on diaphragm fatigue. J. Appl. Physiol. 1990, 68:2107-2113.
63. Singh S.P., Wishnok J.S., Keshive M., Deen W.M., Tannenbaum S.R. The chemistry of the S-nitrosoglutathione/glutathione system. Proc. Natl. Acad. Sci. U.S.A. 1996, 93:14428-14433.
64. Steffen M., Sarkela T.M., Gybina A.A., Steele T.W., Trasseth N.J., Kuehl D., Giulivi C. Metabolism of S-nitrosoglutathione in intact mitochondria. Biochem. J. 2001, 356:395-402.
65. Taylor E.L., Winyard P.G. S-nitrosothiols in disease. Oxidative Stress: Clinical and Biomedical Implications 2007, 157-170. Nova Science Publishers Inc., New York. B.M. Matata, M.M. Eliah (Eds.).
66. Teixeira V., Valente H., Casal S., Marques F., Moreira P. Antioxidant status, oxidative stress, and damage in elite trained kayakers and canoeists and sedentary controls. Int. J. Sport Nutr. Exerc. Metab. 2009, 19:443-456.
67. Thomas S.R., Schulz E., Keaney J.F. Hydrogen peroxide restrains endothelium-derived nitric oxide bioactivity - role for iron-dependent oxidative stress. Free Radic. Biol. Med. 2006, 41:681-688.
68. Torta F., Elviri L., Bachi A. Direct and indirect detection methods for the analysis of S-nitrosylated peptides and proteins. Methods Enzymol. 2010, 473:265-280.
69. Travaline J.M., Sudarshan S., Roy B.G., Cordova F., Leyenson V., Criner G.J. Effect of N-acetylcysteine on human diaphragm strength and fatigability. Am. J. Respir. Crit. Care Med. 1997, 156:1567-1571.
70. Westerblad H., Allen D.G. Cellular mechanisms of skeletal muscle fatigue. Adv. Exp. Med. Biol. 2003, 538:563-570.
71. Whipp B.J., Ward S.A., Lamarra N., Davis J.A., Wasserman K. Parameters of ventilatory and gas exchange dynamics during exercise. J. Appl. Physiol. 1982, 52:1506-1513.
72. Whipp B.J., Wasserman K. Oxygen uptake kinetics for various intensities of constant-load work. J. Appl. Physiol. 1972, 33:351-356.
73. 2 uptake kinetics during supra-maximal exercise in humans. J. Physiol. 2004, 561:623-635.
74. Wong P.S., Hyun J., Fukuto J.M., Shirota F.N., DeMaster E.G., Shoeman D.W., Nagasawa H.T. Reaction between S-nitrosothiols and thiols: generation of nitroxyl (HNO) and subsequent chemistry. Biochemistry 1998, 37:5362-5371.
75. Zeng H., Spencer N.Y., Hogg N. Metabolism of S-nitrosoglutathione by endothelial cells. Am. J. Physiol. Heart Circ. Physiol. 2001, 281:432-439.