Identification and differentiation of Cryptosporidium species by capillary electrophoresis single-strand conformation polymorphism

FEMS Microbiology Letters

Volumn 314, Issue 1, 2011, Pages 34-41

  Power, Michelle L ^a   Holley, Marita ^a   Ryan, Una M ^b   Worden, Paul ^a   Gillings, Michael R ^a  

^a MACQUARIE UNIVERSITY   (Australia)

^b MURDOCH UNIVERSITY   (Australia)

Author keywords

18S rRNA gene; Capillary electrophoresis; Cryptosporidium; Species differentiation; SSCP

Indexed keywords

RNA 18S;

AMPLICON; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM FAYERI; CRYPTOSPORIDIUM HOMINIS; CRYPTOSPORIDIUM PARVUM; CRYPTOSPORIDIUM POSSUM; GENE AMPLIFICATION; GENETIC VARIABILITY; GENOTYPE; LETTER; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; PRIORITY JOURNAL; RELIABILITY; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SINGLE STRAND CONFORMATION POLYMORPHISM; SPECIES DIFFERENCE;

ANIMALS; BASE SEQUENCE; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; DNA, PROTOZOAN; ELECTROPHORESIS, CAPILLARY; HUMANS; MOLECULAR SEQUENCE DATA; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; RNA, RIBOSOMAL, 18S;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM HOMINIS; CRYPTOSPORIDIUM PARVUM;

EID: 78650150090     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.02134.x     Document Type: Letter

References (40)

1. Alvarez-Pellitero P & Sitja-Bobadilla A (2002) Cryptosporidium molnari n. sp. (Apicomplexa: Cryptosporidiidae) infecting two marine fish species, Sparus aurata L. and Dicentrarchus labrax L. Int J Parasitol 32: 1007-1021.
2. Cama VA, Ross JM, Crawford S et al. (2007) Differences in clinical manifestations among Cryptosporidium species and subtypes in HIV-infected persons. J Infect Dis 196: 684-691.
3. Fall A, Thompson RCA, Hobbs RP & Morgan-Ryan U (2003) Morphology is not a reliable tool for delineating species within Cryptosporidium. J Parasitol 89: 399-402.
4. Fayer R & Santin M (2009) Cryptosporidium xiaoi N. Sp. (Apicomplexa: Cryptosporidiidae) in sheep (Ovis aries). Vet Parasit 164: 192-200.
5. Fayer R, Morgan U & Upton SJ (2000) Epidemiology of Cryptosporidium: transmission, detection and identification. Int J Parasitol 30: 1305-1322.
6. Fayer R, Trout JM, Xiao L, Morgan UM, Lal AA & Dubey JP (2001) Cryptosporidium canis n. sp. from domestic dogs. J Parasitol 87: 1415-1422.
7. Fayer R, Santin M & Xiao L (2005) Cryptosporidium bovis N. Sp. (Apicomplexa: Cryptosporidiidae) in cattle (Bos taurus). J Parasitol 91: 624-629.
8. Ferrari BC, Power ML & Bergquist PL (2007) Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection. Lett Biotechnol.
9. Gasser RB & Chilton NB (2001) Applications of single-strand conformation polymorphism (SSCP) to taxonomy, diagnosis, population genetics and molecular evolution of parasitic nematodes. Vet Parasitol 101: 201-213.
10. Gasser RB, El-Osta YGA, Prepens S & Chalmers RM (2004) An improved 'cold SSCP' method for the genotypic and subgenotypic characterization of Cryptosporidium. Mol Cellul Probes 18: 329-332.
11. Gibbons-Matthews C & Prescott AM (2003) Intra-isolate variation of Cryptosporidium parvum small ribosomal RNA genes from human hosts in England. Parasitol Res 90: 439-444.
12. Gillings MR, Krishnan S, Worden PJ & Hardwick SA (2008) Recovery of Diverse genes for class 1 integron-integrases from environmental DNA samples. FEMS Microbiol Lett 287: 56-62.
13. Hill NJ, Deane EM & Power ML (2008) Prevalence and genetic characterization of Cryptosporidium isolates from Common Brushtail Possums (Trichosurus vulpecula) adapted to urban settings. Appl Environ Microb 74: 5549-5555.
14. Hutson KS, Styan CA, Beveridge I, Keough MJ, Zhu X, El-Osta YGA & Gasser RB (2004) Elucidating the ecology of bucephalid parasites using a mutation scanning approach. Mol Cellul Probes 18: 139-146.
15. Jex AR, Ryan UM, Ng J, Campbell BE, Xiao L, Stevens M & Gasser RB (2007a) Specific and genotypic identification of Cryptosporidium from a broad range of host species by nonisotopic, SSCP analysis of nuclear ribosomal DNA. Electrophoresis 28: 2818-2825.
16. Jex AR, Whipp M, Campbell BE, Caccio SM, Stevens M, Hogg G & Gasser RB (2007b) A practical and cost-effective mutation scanning-based approach for investigating genetic variation in Cryptosporidium. Electrophoresis 28: 3875-3883.
17. Jirku M, Valigurova A, Koudela B, Krizek J, Modry D & Slapeta J (2008) New species of Cryptosporidium Tyzzer, 1907 (Apicomplexa) from amphibian host: morphology, biology and phylogeny. Folia Parasit 55: 81-94.
18. Le Blancq Sylvie M, Khramstov NV, Zamani F, Upton SJ & Woo TW (1997) Ribosomal gene organisation in Cryptosporidium parvum. Mol Biochem Parasit 90: 463-478.
19. Lin R-Q, Zhu X-Q, Wei D-X et al. (2007) Characterization of Oesophagostomum spp. from pigs in China by PCR-based approaches using genetic markers in the internal transcribed spacers of ribosomal DNA. Parasit Res 101: 351-356.
20. Mahnaz T, Katrin K, Amer A-J, Isabel M, Gabriele S, Safar F & Hossein AM (2006) Leishmania major: genetic heterogeneity of Iranian isolates by single-strand conformation polymorphism and sequence analysis of ribosomal DNA internal transcribed spacer. Acta Tropica 98: 52-58.
21. Morgan UM, Constantine CC, Forbes DA & Thompson RCA (1997) Differentiation between human and animal isolates of Cryptosporidium parvum using rDNA sequencing and direct PCR analysis. J Parasitol 83: 825-830.
22. Morgan UM, Sargent KD, Deplazes P et al. (1998) Molecular characterisation of Cryptosporidium from various hosts. Parasitology 117: 31-37.
23. Morgan UM, Deplazes P, Forbes DA et al. (1999) Sequence and PCR-RFLP analysis of the internal transcribed spacers of the rDNA repeat unit in isolates of Cryptosporidium from different hosts. Parasitology 118: 49-58.
24. O'Brien E, McInnes L & Ryan U (2008) Cryptosporidium GP60 genotypes from humans and domesticated animals in Australia, North America and Europe. Exp Parasit 118: 118-121.
25. Patel S, Pedraza-Diaz S & McLauchlin J (1999) The identification of Cryptosporidium species and Cryptosporidium parvum directly from whole faeces analysis of a multiplex PCR of the 18S rRNA gene and by PCR/RFLP of the Cryptosporidium outer wall protein (COWP) gene. Int J Parasitol 29: 1241-1247.
26. Power ML & Ryan UM (2008) A new species of Cryptosporidium (Apicomplexa: Cryptosporidiidae) from eastern grey kangaroos (Macropus giganteus). J Parasitol 94: 1114-1117.
27. Power ML, Slade MB, Sangster NC & Veal DA (2004) Genetic characterisation of Cryptosporidium from a wild populations of eastern grey kangaroos Macropus giganteus inhabiting a water catcment. Infect Genet Evol 4: 56-97.
28. Ryan UM, Samarasinghe B, Read C, Buddle JR, Robertson ID & Thompson RCA (2003a) Identification of a novel Cryptosporidium genotype in pigs. Appl Environ Microb 69: 3970-3974.
29. Ryan UM, Xiao L, Lal A, Fayer R & Thompson RCA (2003b) How many species of Cryptosporidium are there? Infect Genet Evol 2.
30. Ryan UM, Xiao L, Read C et al. (2003c) A redescription of Cryptosporidium galli Pavlasek, 1999 (Apicomplexa: Cryptosporidiidae) from birds. J Parasitol 89: 809-813.
31. Ryan UM, Power M & Xiao L (2008) Cryptosporidium fayeri n. sp (Apicomplexa: Cryptosporidiidae) from the Red Kangaroo (Macropus rufus). J Eukaryot Microb 55: 22-26.
32. Spano F, Putignani L, McLauchlin J, Casemore DP & Crisanti A (1997) PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin. FEMS Microbiol Lett 150: 209-217.
33. Sulaiman IM, Morgon U, Arrowood MJ, Thompson RCA, Fayer R, Lal AA & Xiao L (1999) Nucleotide sequence characterization of HSP70 heat-shock gene differentiates various Cryptosporidium species and genotypes of Cryptosporidium parvum. SO - Abstracts of the General Meeting of the American Society for Microbiology, 99, 1999, p. 178.
34. Sulaiman IM, Lal AA & Xiao L (2000) Phylogenetic analysis and evolutionary relationships of Cryptosporidium parasites at the actin gene locus. Am J Trop Med Hyg 62: 257-258.
35. Thompson JD, Higgins DG & Gibson TJ (1994) CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680.
36. Waldron L, Ferrari BC, Gillings MR & Power ML (2009) Terminal restriction length polymorphism for identification of Cryptosporidium species in human faeces. Appl Environ Microb 75: 108-112.
37. Xiao L, Limor JR, Li L, Morgan U, Thompson RCA & Lal AA (1999a) Presence of heterogeneous copies of the small subunit rRNA gene in Cryptosporidium parvum human and marsupial genotypes and Cryptosporidium felis. J Eukaryot Microb 46: 44S-45S.
38. Xiao L, Morgan UM, Limor J et al. (1999b) Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Appl Environ Microb 65: 3386-3391.
39. Xiao L, Sulaiman IM, Ryan UM et al. (2003) Host adaptation and host-parasite co-evolution in Cryptosporidium: implications for taxonomy and public health. Int J Parasitol 32: 1773-1785.
40. Zhou L, Fayer R, Trout JM, Ryan UM, Schaefer FW III & Xiao L (2004) Genotypes of Cryptosporidium species infecting fur-bearing mammals differ from those of species infecting humans. Appl Environ Microb 70: 7574-7577.