Detection of mycoplasma contamination in cell substrates using reverse transcription-PCR assays

Journal of Applied Microbiology

Volumn 110, Issue 1, 2011, Pages 54-60

  Peredeltchouk, M ^a   Wilson David, S A ^a   Bhattacharya, B ^a   Volokhov, D V ^a   Chizhikov, V ^a  

^a CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

Author keywords

16S rRNA; Detection; Mycoplasma; PCR; Reverse transcription

Indexed keywords

ASSAYS; CELL CULTURE; INDUSTRIAL LABORATORIES; INDUSTRIAL RESEARCH; POLYMERASE CHAIN REACTION; RESEARCH LABORATORIES; RNA; TRANSCRIPTION;

16S RRNA; CELL SUBSTRATES; DETECTION; GENOME EQUIVALENT; LIMIT OF DETECTION; MYCOPLASMA; PCR; PCR ASSAY; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION PCR;

CONTAMINATION;

BACTERIAL DNA; RNA 16S;

BACTERIUM; DETECTION METHOD; GENE EXPRESSION; GROWTH RATE; MOLECULAR ANALYSIS; POLYMERASE CHAIN REACTION; REAL TIME;

ACHOLEPLASMA LAIDLAWII; ARTICLE; BACTERIAL GROWTH; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; BACTERIUM DETECTION; MYCOPLASMA; MYCOPLASMA ARGININE; MYCOPLASMA FERMENTANS; MYCOPLASMA HYORHINIS; MYCOPLASMA ORALE; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ACHOLEPLASMA LAIDLAWII; BACTERIA (MICROORGANISMS); MYCOPLASMA; MYCOPLASMA ARGININI;

EID: 78650129284     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2010.04853.x     Document Type: Article

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