High-level expression, purification, crystallization and preliminary X-ray crystallographic studies of the receptor-binding domain of botulinum neurotoxin serotype D

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volumn 66, Issue 12, 2010, Pages 1610-1613

  Zhang, Yanfeng ^a   Gao, Xiaoli ^a   Qin, Ling ^b   Buchko, Garry W ^a   Robinson, Howard ^c   Varnum, Susan M ^a  

^a PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^b SANDIA NATIONAL LABORATORIES   (United States)

^c BROOKHAVEN NATIONAL LABORATORY   (United States)

Author keywords

botulinum neurotoxin; codon optimization; receptor binding domain

Indexed keywords

BOTULINUM TOXIN; BOTULINUM TOXIN TYPE D;

ARTICLE; CHEMISTRY; CLOSTRIDIUM BOTULINUM; CRYSTALLIZATION; GEL CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; WESTERN BLOTTING; X RAY CRYSTALLOGRAPHY;

BLOTTING, WESTERN; BOTULINUM TOXINS; CHROMATOGRAPHY, GEL; CLOSTRIDIUM BOTULINUM; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MASS SPECTROMETRY; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY;

ANIMALIA; ESCHERICHIA COLI;

EID: 78650117392     PISSN: 17443091     EISSN: 17443091     Source Type: Journal    
DOI: 10.1107/S1744309110039874     Document Type: Article

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