A homogeneous, high-throughput fluorescence anisotropy-based DNA supercoiling assay

Journal of Biomolecular Screening

Volumn 15, Issue 9, 2010, Pages 1088-1098

  Shapiro, Adam ^a   Jahic, Haris ^a   Prasad, Swati ^a   Ehmann, David ^a   Thresher, Jason ^a   Gao, Ning ^a   Hajec, Laurel ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

fluorescence anisotropy; gyrase; high throughput screening; supercoiling; topoisomerase

Indexed keywords

DNA TOPOISOMERASE; DNA TOPOISOMERASE (ATP HYDROLYSING); NALIDIXIC ACID; OLIGONUCLEOTIDE; PLASMID DNA; QUINOLINE DERIVED ANTIINFECTIVE AGENT;

ANALYTIC METHOD; ANISOTROPY; ARTICLE; DNA SUPERCOILING; ENZYME INHIBITION; ESCHERICHIA COLI; FLUORESCENCE; GEL ELECTROPHORESIS; GENE EXPRESSION; HIGH THROUGHPUT SCREENING; NONHUMAN; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; QUANTITATIVE ANALYSIS;

BIOCATALYSIS; DNA GYRASE; DNA TOPOISOMERASES, TYPE I; DNA, SUPERHELICAL; ELECTROPHORESIS, AGAR GEL; FLUORESCENCE POLARIZATION; HIGH-THROUGHPUT SCREENING ASSAYS; HYDROGEN-ION CONCENTRATION; MAGNESIUM; NUCLEIC ACID CONFORMATION; OLIGONUCLEOTIDES; PLASMIDS;

ESCHERICHIA COLI;

EID: 78650110985     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057110378624     Document Type: Article

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