Hypoxia-induced retinopathy model in adult zebrafish

Nature Protocols

Volumn 5, Issue 12, 2010, Pages 1903-1910

  Cao, Ziquan ^a   Jensen, Lasse D ^b   Rouhi, Pegah ^b   Hosaka, Kayoko ^b   Länne, Toste ^a   Steffensen, John F ^c   Wahlberg, Erik ^a   Cao, Yihai ^b  

^a LINKÖPING UNIVERSITY   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT ANIMAL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CLINICAL PROTOCOL; CONFOCAL MICROSCOPY; DISSECTION; HYPOXIA; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; PROCESS DESIGN; RETINA NEOVASCULARIZATION; TISSUE PREPARATION; ZEBRA FISH;

ANIMALS; ANOXIA; DIABETIC RETINOPATHY; DISEASE MODELS, ANIMAL; GREEN FLUORESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; NEOVASCULARIZATION, PATHOLOGIC; ZEBRAFISH;

ANIMALIA; DANIO RERIO;

EID: 78649928382     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.149     Document Type: Article

References (12)

1. Boutin, A. T. et al. Epidermal sensing of oxygen is essential for systemic hypoxic response. Cell 133, 223-234 (2008).
2. Cao, Y. Monotherapy versus combination therapy of angiogenic and arteriogenic factors for the treatment of ischemic disorders. Curr. Mol. Med. 9, 967-972 (2009).
3. Makino, Y. et al. Inhibitory PAS domain protein is a negative regulator of hypoxia-inducible gene expression. Nature 414, 550-554 (2001).
4. Kondo, T. et al. Knockout of insulin and IGF-1 receptors on vascular endothelial cells protects against retinal neovascularization. J. Clin. Invest. 111, 1835-1842 (2003).
5. Cao, R., Jensen, L. D., Soll, I., Hauptmann, G. & Cao, Y. Hypoxia-induced retinal angiogenesis in zebrafish as a model to study retinopathy. PLoS One 3, e2748 (2008).
6. Lee, S. L. et al. Hypoxia-induced pathological angiogenesis mediates tumor cell dissemination, invasion, and metastasis in a zebrafish tumor model. Proc. Natl Acad. Sci. USA 106, 19485-19490 (2009).
7. Rouhi, P. et al. Pathological angiogenesis facilitates tumor cell dissemination and metastasis. Cell Cycle 9, 913-917 (2010).
8. Dahl Ejby Jensen, L. et al. Nitric oxide permits hypoxia-induced lymphatic perfusion by controlling arterial-lymphatic conduits in zebrafish and glass catfish. Proc. Natl Acad. Sci. USA 106, 18408-18413 (2009).
9. Lawson, N. D. & Weinstein, B. M. In vivo imaging of embryonic vascular development using transgenic zebrafish. Dev. Biol. 248, 307-318 (2002).
10. Gariano, R. F. & Gardner, T. W. Retinal angiogenesis in development and disease. Nature 438, 960-966 (2005).
11. Fouquet, B., Weinstein, B. M., Serluca, F. C. & Fishman, M. C. Vessel patterning in the embryo of the zebrafish: guidance by notochord. Dev. Biol. 183, 37-48 (1997).
12. Alvarez, Y. et al. Genetic determinants of hyaloid and retinal vasculature in zebrafish. BMC Dev. Biol. 7, 114(2007).