Ocimum sanctum essential oil and its active principles exert their antifungal activity by disrupting ergosterol biosynthesis and membrane integrity

Research in Microbiology

Volumn 161, Issue 10, 2010, Pages 816-823

  Khan, Amber ^a   Ahmad, Aijaz ^a   Akhtar, Feroz ^b   Yousuf, Snowber ^a   Xess, Immaculata ^c   Khan, Luqman A ^a   Manzoor, Nikhat ^a  

^a JAMIA MILLIA ISLAMIA CENTRAL UNIVERSITY   (India)

^b JAWAHARLAL NEHRU UNIVERSITY   (India)

^c ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Candida; Ergosterol; Fluconazole; Ocimum sanctum; Propidium iodide

Indexed keywords

ALPHA FARNESENE; BETA FARNESENE; BETA PINENE; BOURBONENE; CAPRYLIC ALCOHOL; CAPRYLYL ACETATE; CARVEOL; CARVONE; CARYOPHYLLENE; CINEOLE; CYCLONOL; ERGOSTEROL; ESSENTIAL OIL; ESTRAGOLE; GERANIOL; GERMACRENE D; ISOMENTHONE; LIMONENE; LINALOLOXIDE; LINALOOL; MENTHOL; MENTHONE; MYRCENE; O CYMENE; OCIMUM SANCTUM ESSENTIAL OIL; PHTHALIC ACID; PINENE; PLANT MEDICINAL PRODUCT; PROPIDIUM IODIDE; TERPINENE; UNCLASSIFIED DRUG;

ANTIFUNGAL ACTIVITY; ARTICLE; CANDIDA; CELL MEMBRANE; CHEMICAL COMPOSITION; CONTROLLED STUDY; DRUG MECHANISM; FLOW CYTOMETRY; FUNGAL STRAIN; FUNGICIDAL ACTIVITY; FUNGUS ISOLATION; MASS FRAGMENTOGRAPHY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; STEROL SYNTHESIS; YEAST CELL;

ANTIFUNGAL AGENTS; BIOSYNTHETIC PATHWAYS; CANDIDA; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; ERGOSTEROL; FLOW CYTOMETRY; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; MICROBIAL SENSITIVITY TESTS; MICROSCOPY, CONFOCAL; OCIMUM; OILS, VOLATILE; PROPIDIUM; STAINING AND LABELING;

CANDIDA; OCIMUM TENUIFLORUM;

EID: 78649911072     PISSN: 09232508     EISSN: 17697123     Source Type: Journal    
DOI: 10.1016/j.resmic.2010.09.008     Document Type: Article

References (35)

1. Ahmad A., Khan A., Manzoor N., Khan L.A. Evolution of ergosterol biosynthesis inhibitors as fungicidal against Candida. Microb. Pathog. 2010, 48:35-41.
2. Arthington-Skaggs B.A., Adi H., Desai T., Morrison C.J. Quantification of ergosterol content: novel method for determination of fluconazole susceptibility of Candida albicans. J. Clin. Microbiol. 1999, 37:3332-3337.
3. Bakkali F., Averbeck S., Averbeck D., Idaomar M. Biological effects of essential oils - a review. Food Chem. Toxicol. 2008, 46:446-475.
4. Bansod S., Rai M. Antifungal activity of essential oils from Indian medicinal plants against human pathogenic Aspergillus fumigatus and A.niger. World J. Med. Sci. 2008, 3(2):81-88.
5. Burt S. Essential oils: their antibacterial properties and potential applications in foods - a review. Int. J. Food Microbiol. 2004, 94:223-253.
6. Cavaleiro C., Pinto E., Goncalves M.J., Salgueiro L.R. Antifungal activity of Juniperus essential oils against dermatophyte, Aspergillus and Candida strains. J. Appl. Microbiol. 2006, 100:1333-1338.
7. CLSI Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts 2008, Approved Standard M27-A3, Clinical and Laboratory Standards Institute, Wayne, PA. third ed.
8. Cohen B.E. Amphotericin B toxicity and lethality: a tale of two channels. Int. J. Pharm. 1998, 162:95-106.
9. Cristani M., D'Arrigo M., Mandalari G., Castelli F., Sarpietro M.G., Micieli D., Venuti V., Bisignano G., Saija A., Trombetta D. Interaction of four monoterpenes contained in essential oils with model membranes: implications for their antibacterial activity. J. Agric. Food Chem. 2007, 55(15):6300-6308.
10. Dharmagadda V.S.S., Tandon M., Vasudevan P. Biocidal activity of the essential oils of Lantana camara, Ocimum sanctum and Tagetes patula. J. Sci. Ind. Res. 2005, 64:53-56.
11. Eugenia P., Vale-Silva L., Cavaleiro C., Salgueiro L. Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species. J. Med. Microbiol. 2009, 58:1454-1462.
12. Grover G., Rao S. Investigations of antimicrobial efficiency from essential oil from O. sanctum & O. gratissimum. Perfuma, Kosmat 1977, 58:326-329.
13. Iwu M.W., Duncan A.R., Okunji C.O. New antimicrobials of plant origin. Perspectives on New Crops and New Uses 1999, 457-462. ASHS Press, Alexandria. J. Janick (Ed.).
14. Kalemba D., Kunicka A. Antibacterial and antifungal properties of essential oils. Curr. Med. Chem. 2003, 10:813-829.
15. Khan A., Ahmad A., Xess I., Khan A.L. Manzoor, N. Anticandidal effect of Ocimum sanctum essential oil and its synergy with fluconazole and ketoconazole. Phytomedicine 2010, 17:921-925.
16. Kumar A., Shukla R., Singh P., Dubey N.K. Chemical composition, antifungal and antiaflatoxigenic activities of Ocimum sanctum L. essential oil and its safety assessment as plant based antimicrobial. Food Chem. Toxicol. 2010, 48(2):539-543.
17. Laskar S., Majumdar S.G. Variation of major constituents of essential oil of the leaves of Ocimum sanctum Linn. J. Indian Chem. Soc. 1988, 65:301-302.
18. Lucchini J.J., Corre J., Cremieux A. Antibacterial activity of phenolic compounds and aromatic alcohols. Res. Microbiol. 1990, 141:499-510.
19. Lupetti A., Danesi R., Campa M., Del Tacca M., Kelly S. Molecular basis of resistance to azole antifungals. Trends Mol. Med. 2002, 8:76-81.
20. Maschmeyer G. The changing epidemiology of invasive fungal infections: new threats. Int. J. Antimicrob. Agents 2006, 27S:S3-S6.
21. Nweze E.I., Eze E.E. Justification for the use of Ocimum gratissimum L in herbal medicine and its interaction with disc antibiotics. BMC Complement. Altern. Med. 2009, 28:9-37.
22. Pfaller M.A., Diekema D.J. Rare and emerging opportunistic fungal pathogens: concern for resistance beyond Candida albicans and Aspergillus fumigatus. J. Clin. Microbiol. 2004, 42(10):4419-4431.
23. Pina-Vaz C., Rodrigues A.G., Pinto E., Costa-de-Oliveira S., Tavares C., Salgueiro L., Cavaleiro C., Goncalves M.J., Martinezde-Oliveira J. Antifungal activity of Thymus oils and their major compounds. J. Eur. Acad. Dermatol. Venereol. 2004, 18:73-78.
24. Pina-Vaz C., Sansonetty F., Rodrigues A., Costa-de-Oliveira S., Martinez-de-Oliveira J., Fonseca A.F. Cytometric approach for rapid evaluation of susceptibility of Candida strains to antifungals. Clin. Microbiol. Infect. 2001, 7:609-618.
25. Prakash P., Gupta N. Therapeutic uses of Ocimum sanctum Linn (Tulsi) with a note on eugenol and its pharmacological actions: a short review. Indian J. Physiol. Pharmacol. 2005, 49(2):125-131.
26. Rajeshwari S. Ocimum sanctum. The Indian home remedy. Current Medical Scene 1992, Bombay Central, Bombay.
27. Raseetha V.S., Cheng S.F., Chuah C.H. Comparative study of volatile compounds from genus Ocimum. Am. J. Appl. Sci. 2009, 6(3):523-528.
28. Rosato A., Cesare V., Daniela G., Balenzano L., Mallamaci R. The inhibition of Candida species by selected essential oils and their synergism with amphotericin B. Phytomedicine 2008, 15,635-15,638.
29. Sanglard D., Ischer F., Parkinson T., Falconer D., Bille J. Candida albicans mutations in the ergosterol biosynthetic pathway and resistance to several antifungal agents. Antimicrob. Agents Chemother. 2003, 47:2404-2412.
30. Sethi J., Sood S., Seth S., Thakur A. Protective effect of Tulsi (Ocimum sanctum) on lipid peroxidation in stress induced by anemic hypoxia in rabbits. Indian J. Physiol. Pharmacol. 2003, 47:115-119.
31. Shimokawa O., Kato Y., Kawano K. Accumulation of 14α-methylergosta-8, 24(28)-dien-3b, 6a-diol in 14α-demethylation mutant of Candida albicans: genetic evidence for the involvement of 5-desaturase. Biochim. Biophys. Acta 1989, 1003:15-19.
32. Shimokawa O., Kato Y., Nakayama H. Accumulation of 14-methyl sterols and defective hyphal growth in Candida albicans. J. Med. Vet. Mycol. 1986, 24:327-336.
33. Vanden Bossche H. Biochemical targets for antifungal azole derivatives: hypothesis on the mode of action. Curr. Top. Med. Mycol. 1985, 1:313-351.
34. Xu J., Zhou F., Ji B.P., Pei R.S., Xu N. The antibacterial mechanism of carvacrol and thymol against Escherichia coli. Lett. Appl. Microbiol. 2008, 47(3):174-179.
35. Zheljazkov V.D., Cantrell C.L., Tekwani B., Khan S.I. Content, composition, and bioactivity of the essential oils of three basil genotypes as a function of harvesting. J. Agric. Food Chem. 2008, 56:380-385.