Cryopreservation of sweetpotato (Ipomoea batatas) and its pathogen eradication by cryotherapy

Biotechnology Advances

Volumn 29, Issue 1, 2011, Pages 84-93

  Feng, Chaohong ^a   Yin, Zhenfang ^a   Ma, Yanli ^a   Zhang, Zhibo ^a   Chen, Long ^a   Wang, Biao ^a   Li, Baiquan ^a   Huang, Yushen ^a   Wang, Qiaochun ^a  

^a NORTHWEST A F UNIVERSITY   (China)

Author keywords

Cryopreservation; Cryotherapy; Embryogenesis; Ipomoea batatas; Pathogen eradication; Phytoplasma; Shoot regeneration; Shoot tips; Sweetpotato; Virus

Indexed keywords

CRYO-PRESERVATION; EMBRYOGENESIS; IPOMOEA BATATAS; PHYTOPLASMAS; SHOOT REGENERATION; SHOOT TIPS; SWEET POTATO;

BIOLOGICAL MATERIALS PRESERVATION; CRYOTHERAPY; DEVELOPING COUNTRIES; ECOLOGY; GENETIC ENGINEERING; HISTOLOGY; VIRUSES;

PATHOGENS;

CROP PRODUCTION; CROP YIELD; CRYOPRESERVATION; DEVELOPING WORLD; DISEASE CONTROL; EMBRYONIC DEVELOPMENT; GENETIC ENGINEERING; GENETIC RESOURCE; GERMPLASM; PATHOGEN; PHYTOPLASMA; PLANT BREEDING; REGENERATION; SHOOT; SUGAR BEET; SUSTAINABILITY;

COLD; CRYOPRESERVATION; CRYOTHERAPY; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; PHYSIOLOGY; PHYTOPLASMA; PLANT; PLANT DISEASE; PLANT VIRUS; PRENATAL DEVELOPMENT; REVIEW; SWEET POTATO; VIROLOGY;

COLD TEMPERATURE; CRYOPRESERVATION; CRYOTHERAPY; IPOMOEA BATATAS; PHYTOPLASMA; PLANT DISEASES; PLANT SHOOTS; PLANT VIRUSES;

CANDIDATUS PHYTOPLASMA; IPOMOEA BATATAS; MANIHOT ESCULENTA; TRITICUM AESTIVUM; ZEA MAYS;

EID: 78649833036     PISSN: 07349750     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biotechadv.2010.09.002     Document Type: Review

References (115)

1. Al-Mazrooei S., Bhatti M.H., Henshaw G.G., Taylor N.J., Blakesley D. Optimization of somatic embryogenesis in fourteen cultivars of sweet potato. Plant Cell Rep 1997, 16:710-714.
2. Bajaj Y.P.S. Storage and cryopreservation of in vitro cultures. Biotechnology in agriculture and forestry 1991, vol. 17:361-381. Springer, Berlin, Heidelberg, New York. Y.P.S. Bajaj (Ed.).
3. Benson E.E. Cryopreservation of phytodiversity: a critical appraisal of theory & practice. Curr Rev Plant Sci 2008, 27:141-219.
4. Bhatti M.H., Percival T., Davey C.D.M., Henshaw G.G., Blakesley D. Cryopreservation of embryogenic tissue of a range of genotypes of sweet potato (Ipomoea batatas [L] Lam.) using an encapsulation protocol. Plant Cell Rep 1997, 16:802-806.
5. Bieniek M.E., Harrell R.C., Cantliffe D.J. Enhancement of somatic embryogenesis of Ipomoea batatas in solid cultures and production of mature somatic embryos in liquid cultures for application to a bioreactor production system. Plant Cell Tissue Organ Cult 1995, 41:1-8.
6. Blakesley D., AI-Mazrooei S., Henshaw G.G. Cryopreservation of embryogenic tissue of sweet potato (Ipomoea batatas): use of sucrose and dehydration for cryoprotection. Plant Cell Rep 1995, 5:259-263.
7. Blakesley D., Al Mazrooei S., Bhatti M.H., Henshaw G.G. Cryopreservation of non-encapsulated embryogenic tissue of sweet potato (Ipomoea batatas). Plant Cell Rep 1996, 15:873-876.
8. Blakesley D., Percival T., Bhatti M.H., Henshaw G.G. A simplified protocol for cryopreservation of embryogenic tissues of sweet potato (Ipomoea batatas (L.) Lam.) utilizing sucrose preculture only. Cryo Lett 1997, 8:77-80.
9. Brison M., de Boucaud M.T., Pierronnet A., Dosba F. Effect of cryopreservation on the sanitary state of a cv. Prunus rootstock experimentally contaminated with plum pox potyvirus. Plant Sci 1997, 123:189-196.
10. Burritt D.J. Efficient cryopreservation of adventitious shoots of Begonia x erythrophylla using encapsulation-dehydration requires pretreatment with both ABA and proline. Plant Cell Tissue Organ Cult 2008, 95:209-215.
11. Cavalcante Alves J.M., Sihachakr D., Allot M., Tizroutine S., Mussio I., Servaes A., et al. Isozyme modifications and plant regeneration through somatic embryogenesis in sweet potato (Ipomoea batatas (L.) Lam.). Plant Cell Rep 1994, 13:437-441.
12. Chée R.P., Cantliffe D.J. Somatic embryony patterns and plant regeneration in Ipomoea batatas Poir. In Vitro Cell Dev Biol Plant 1988, 24:955-958.
13. Chée R.P., Cantliffe D.J. Selective enhancement of Ipomoea batatas Poir. embryogenic and non-embryogenic callus growth and production of embryos in liquid culture. Plant Cell Tissue Organ Cult 1988, 15:149-159.
14. Chée R.P., Cantliffe D.J. Composition of embryogenic suspension cultures of Ipomoea batatas Poir. and production of individualized embryos. Plant Cell Tissue Organ Cult 1989, 17:39-52.
15. Chée R.P., Leskovar D.I., Cantliffe D.J. Optimizing embryogenic callus and embryo growth of a synthetic seed system for sweetpotato by varying media nutrient concentrations. J Am Soc Hortic Sci 1992, 117:663-667.
16. Chen L., Bhagsari A., Carter J. Effects of medium composition and culture duration on in vitro morphogenesis of sweet potato. Biol Plant 2006, 50:114-117.
17. Choi H.J., Chandrasekhar Th., Lee H.-Y., Kim K.-M. Production of herbicide-resistant transgenic sweet potato plants through Agrobacterium tumefaciens method. Plant Cell Tissue Organ Cult 2007, 91:235-242.
18. Danso K.E., Ford-Llyod B.V. The effect of abscisic acid and sucrose on post-thaw embryogenic competence and subsequent plant recovery from embryogenic calli of cassava. Am Eur J Agric Environ Sci 2008, 3:663-671.
19. Decruse S.W., Seeni S. Ammonium nitrate in the culture medium influences regeneration potential of cryopreserved shoot tips of Holostemma annulare. Cryo Lett 2002, 25:55-60.
20. Decruse S.W., Seeni S., Nair G.M. Preparative procedures and culture medium affect the success of cryostorage of Holostemma annulare shoot tips. Plant Cell Tissue Organ Cult 2004, 76:179-182.
21. Dereuddre J., Scottez C., Amud Y., Duron M. Resistance of alginate-coated axillary shoot tips of pear tree (Pyrus communis L. cv. 'Beurre Hardy') in vitro plantlets to dehydration and subsequent freezing in liquid nitrogen: effects of previous cold hardening. C R Acad Sci Paris Ser III 1990, 310:317-323.
22. Desamero N.V., Rhodes B.B., Decoteau D.R., Bridges W.C. Picolinic acid-induced direct somatic embryogenesis in sweet potato. Plant Cell Tissue Organ Cult 1994, 37:103-111.
23. Ding F., Jin S.X., Hong N., Zhong Y., Cao Q., Yi G.J., et al. Vitrification-cryopreservation, an efficient method for eliminating Candidatus Liberobacter asiaticus, the citrus Huanglongbing pathogen, from in vitro adult shoot tips. Plant Cell Rep 2008, 27:241-250.
24. Engelmann F. In vitro conservation methods. Biotechnology and plant genetic resources 1997, 119-161. CAB International, Oxford, UK. J.A. Callow, B.V. Ford-Lloyd, H.J. Newbury (Eds.).
25. Engelmann F., Takagi H. Cryopreservation of tropical germplasm: current research progress and application 2000, IPGRI, Rome.
26. Engelmann F., Gonzalez-Arnao M.-T., Wu Y.J., Escobar R. The development of encapsulation dehydration. Plant cryopreservation: a practical guide 2008, 59-75. Springer, Berlin, Germany. R.M. Reed (Ed.).
27. Fabre J., Dereuddre J. Encapsulation-dehydration, a new approach to cryopreservation of Solanum shoot tips. Cryo Lett 1990, 11:413-426.
28. Faccioli V.C., Marani F. Virus elimination by meristem tip culture and tip micrografting. Plant virus disease control 1998, 346-380. APS Press, St Paul, MN, USA. A. Hadidi, R.K. Khetarpal, H. Koganezawa (Eds.).
29. Fahy G.M., MacFarlande D.R., Angell C.A., Meryman H.T. Vitrification as an approach to cryopreservation. Cryobiology 1984, 21:413-426.
30. Gaba V., Singer S. Propagation of sweetpotato, in situ germplasm conservation and conservation by tissue culture. The sweetpotato 2009, 65-80. Springer. G. Loebenstein, G. Thottappilly (Eds.).
31. Gama M.I.C.S., Leite R.P., Cordeiro A.R., Daniel J., Cantliffe D.J. Transgenic sweet potato plants obtained by Agrobacterium tumefaciens-mediated transformation. Plant Cell Tissue Organ Cult 1996, 46:231-244.
32. Gao F., Gong Y.F., Zhang P.B. Production and development of virus-free sweetpotato in China. Crop Prot 2000, 19:105-111.
33. Gibson R.W., Mpembe I., Alicai T., Carey E.E., Mwanga R.O.M., Seal S.E., et al. Symptoms, aetiology and serological analysis of sweet potato virus disease in Uganda. Plant Pathol 1998, 47:95-102.
34. Golmirzaie A.M., Panta A., Diaz S. Systematic determination of an adequate method for large-scale sweetpotato cryopreservation at CIP. JIRCAS Int Agric Ser 2000, 8:460-462.
35. Gutiérrez D.L., Fuentes S., Salazar L.F. Sweetpotato virus disease (SPVD): distribution, incidence, and effect on sweetpotato yield in Peru. Plant Dis 2003, 87:297-302.
36. He H.Z., Han Y.F., Wang Y.P., Zhai H., Liu Q.C. In vitro selection and identification of sweetpotato (Ipomoea batatas (L.) Lam.) plants tolerant to NaCl. Plant Cell Tissue Organ Cult 2009, 96:69-74.
37. Helliot B., Panis B., Poumay Y., Swenen R., Lepoivre P., Frison E. Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.). Plant Cell Rep 2002, 20:1117-1122.
38. Helliot B., Swenen R., Poumay Y., Frison E., Lepoivre P., Panis B. Ultrastructural changes associated with cryopreservation of banana (Musa spp.) highly proliferating meristems. Plant Cell Rep 2003, 21:690-698.
39. Hirai D., Sakai A. Simplified cryopreservation of sweetpotato [(Ipomoea batatas (L) Lam.] by optimizing conditions for osmoprotection. Plant Cell Rep 2003, 21:961-966.
40. Holmes F.O. Elimination of spotted wilt from a stock of dahlia. Phytopathology 1948, 38:314.
41. Jarret R.L., Salazar S., Fernandez Z.R. Somatic embryogenesis in sweet potato. HortScience 1984, 19:397-398.
42. Jenderek M.M., Skogerboe D., Ellis D. Improvement of cryopreservation technique for long-term storage of shoot tips of Ipomoea batatas. In Vitro Cell Dev Biol Anim 2008, 44:S69.
43. Kaczmarczyk A., Rutten T., Melzer M., Keller E.R.J. Ultrastrctural changes associated with cryopreservation of potato (Solanum tuberosum L.) shoot tips. Cryo Lett 2008, 29:145-156.
44. Kartha K.K., Leung N.L., Moroginski L. In vitro growth and plant regeneration from cryopreserved meristems of cassava (Manihot esculenta Crantz.). Z Pflanzenphysiol 1982, 107S:133-140.
45. Karyeija R.F., Gibson R.W., Valkonen J.P.T. The significance of sweet potato feathery mottle virus in subsistence sweet potato production in Africa. Plant Dis 1998, 82:4-15.
46. Karyeija R.F., Kreuze J.F., Gibson R.W., Valkonen J.P.T. Synergistic interactions of a potyvirus and a phloem-limited crinivirus in sweet potato plants. Virology 2000, 269:26-36.
47. Kim Y.-H., Soon L., Yang K.-S., Kim C.Y., Kwon S.-Y., Lee H.-S., et al. Expression of Arabidopsis NDPK2 increases antioxidant enzyme activities and enhances tolerance to multiple environmental stresses in transgenic sweetpotato plants. Mol Breed 2009, 24:233-244.
48. Kreuze J.F., Klein I.S., Lazaro M.Y., Cuellar W.J., Morgan G.L., Mejia P.G.C., et al. RNA silencing-mediated resistance to a crinivirus (Closteroviridae) in cultivated sweetpotato (Ipomoea batatas L.) and development of sweetpotato virus disease following co-infection with a potyvirus. Mol Plant Pathol 2008, 5:589-598.
49. Kreuze J.K., Valkonen J.P.T., Ghislain M. Genetic engineering. The sweetpotato 2009, 41-63. Springer. G. Loebenstein, G. Thottappilly (Eds.).
50. Kuriyama A., Watanabe K., Ueno S., Mitsuda H. Inhibitory effect of ammonium ion on recovery of cryopreserved rice cells. Plant Sci 1989, 64:231-235.
51. Kuriyama A., Watanabe K., Kawata K., Kawai F., Kanamori M. Sensitivity of cryopreserved Lavandula vera cells to ammonium ion. J Plant Physiol 1996, 148:693-695.
52. Liu J.R., Cantliffe D.J. Somatic embryogenesis and plant regeneration in tissue cultures of sweet potato (Ipomoea batatas Poir.). Plant Cell Rep 1984, 3:12-115.
53. Liu Q.C., Kokubu T., Sato M. Varietal differences of somatic embryogenesis in shoot tip culture of sweetpotato, Ipomoea batatas (L.) Lam. Jpn J Breed 1992, 42(suppl. 2):8-9.
54. Liu Q.C., Luo J.Q., Zhou H.Y., Lu S.Y. High frequency somatic embryogenesis and plant regeneration in sweetpotato, Ipomoea batatas (L.) Lam. J Agric Biotechnol 1993, 1:84-89. (in Chinese).
55. Liu Q.C., Mi K.X., Lu D.H., Zhou H.Y., Fu Z. Establishment of embryogenic cell suspension cultures in sweetpotato, Ipomoea batatas (L.) Lam. Acta Agric Sin 1997, 23:22-26.
56. Liu Q.C., Zhai H., Wang Y., Zhang D.P. Efficient plant regeneration from embryogenic suspension cultures of sweetpotato. In Vitro Cell Dev Biol Plant 2001, 37:564-567.
57. Loebenstein G. Origin, distribution and economic importance. The sweetpotato 2009, 9-12. Springer. G. Loebenstein, G. Thottappilly (Eds.).
58. Loebenstein G., Fuentes S., Cohen J., Salazar L.F. Sweet potato. Virus and virus-like diseases of major crops in developing countries 2003, 223-248. Kluwer Academic Publishers, Dordrecht. G. Loebenstein, G. Thottappilly (Eds.).
59. Loebenstein G., Thottappilly G., Fuentes S., Cohen J. Virus and phytoplasma diseases. The sweetpotato 2009, 105-134. Springer. G. Loebenstein, G. Thottappilly (Eds.).
60. Luan Y.-S., Zhang J., Gao X.-R., An L.J. Mutation induced by ethylmethanesulphonate (EMS), in vitro screening for salt tolerance and plant regeneration of sweet potato (Ipomoea batatas L.). Plant Cell Tissue Organ Cult 2007, 88:77-81.
61. Martinelli L., Gribaudo L. Somatic embryogenesis in grapevine. Molecular biology & biotechnology of the grapevine 2001, 327-351. Kluwer Academic Publishers, The Netherland. K.A. Roubelakis-Angelakis (Ed.).
62. Milgram M., Cohen J., Loebenstein G. Effects of sweet potato feathery mottle virus and sweet potato sunken vein virus on sweet potato yields and rates of re-infection of virus-free planting materials in Israel. Phytopathology 1996, 24:189-193.
63. Min S.R., Woo J.W., Jeong W.J., Han S.K., Lee Y.B., Liu J.R. Production of human lactoferrin in transgenic cell suspension cultures of sweet potato. Biol Plant 2006, 50:131-134.
64. Mukasa S.B., Rubaihayo P.R., Valkonen J.P.T. Interactions between a crinivirus, an impomovirus and a potyvirus in coinfection sweetpotato plants. Plant Pathol 2006, 53:458-467.
65. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1962, 15:473-479.
66. Ngeve J.M., Bouwkamp J.G. Effects of sweet potato virus disease (SPVD) on the yield of sweet potato genotypes in Cameroon. Exp Agric 1991, 27:221-225.
67. Njeru R.W., Mburu M.W.K., Cheramgon E., Gibson R.W., Obudho E., Yobera D. Studies on the physiological effects of viruses on sweet potato yield in Kenya. Ann Appl Biol 2004, 145:71-76.
68. Olivares-Fuster O., Asíns M.J., Duran-Vila N., Navarro L. Cryopreserved callus: a source of protoplasts for citrus improvement. J Hortic Sci Biotechnol 2000, 75:635-640.
69. Padayachee K., Watt M.P., Edwards N., Mycock D.J. Physiological responses of Eucalyptus in vitro axillary buds to cryopreparative desiccation and osmotic preculture: effects of abscisic acid. S Afr J Bot 2008, 74:639-646.
70. Panis B., Lambardi M. Status of cryopreservation technologies in plants (crops and forest trees). The role of biotechnology in exploring and protecting agricultural genetic resources 2006, 61-78. FAO, Rome, Italy. J. Ruane, A. Sonnino (Eds.).
71. Panis B., Schoofs H., Remy S., Sági L., Swennen R. Cryopreservation of banana embryogenic cell suspensions: an aid for genetic transformation. JIRCAS Int Agric Ser 2000, 8:103-109.
72. Panis B., Piette B., Swennen R. Droplet vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae. Plant Sci 2005, 168:45-55.
73. Panta A., Panis B., Ynouye C., Criel B., Swennen R., Roca W. Improvement of potato cryopreservation for the long-term conservation of Andean landraces at the International Potato Center (CIP). Cryobiology 2006, 53:401.
74. Pearson M.N., Keane P.J., Thiagalingham K. Little leaf: a disease of sweet potato in Papua New Guinea probably caused by mycoplasma-like organisms. Phytopathol Z 1984, 109:269-276.
75. Pennycooke J.C., Towill L.E. Cryopreservation of shoot tips from in vitro plants of sweet potato [Ipomoea batatas (L.) Lam.] by vitrification. Plant Cell Rep 2000, 19:733-737.
76. Pennycooke J.C., Towill L.E. Medium alternations improve regrowth of sweet potato (Ipomoea batatas {L.} Lam.) shoot tips cryopreserved by vitrification and encapsulation-dehydration. Cryo Lett 2001, 22:381-389.
77. Plessis P., Steponkus P.L. Cryopreservation of sweet potato shoot-tips. Cryobiology 1996, 33:655-656.
78. Popova E.V., Lee E.J., Wu C.H., Hahn E.J., Paek K.Y. A simple method for cryopreservation of Ginkgo biloba callus. Plant Cell Tissue Organ Cult 2009, 97:337-343.
79. Reed B.M. Plant cryopreservation: a practical guide 2008, Berlin, Germany, Springer.
80. Ryynänen L.A., Häggman H.M. Substitution of ammonium ions during cold hardening and post-thaw cultivation enhances recovery of cryopreserved shoot tips of Betula pendula. J Plant Physiol 1999, 154:735-742.
81. Ryynänen L., Häggman H.M. Recovery of cryopreserved silver shoot tips is affected by the pre-freezing age of the cultures and ammonium substitution. Plant Cell Rep 2001, 20:354-360.
82. Sakai A., Engelmann F. Vitrification, encapsulation-vitrification and droplet-vitrification: a review. Cryo Lett 2007, 28:151-172.
83. Sakai A., Kobayashi S., Oiyama I. Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. Var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 1990, 9:30-33.
84. Sakai A., Hirai D., Niino T. Development of PVS-based vitrification and encapsulation-vitrification protocols. Plant cryopreservation: a practical guide 2008, 33-57. Springer, Berlin, Germany. R.M. Reed (Ed.).
85. Schaefers G.A., Terry E.R. Insect transmission of sweet potato disease agents in Nigeria. Phytopathology 1976, 66:642-645.
86. Schnabel-Preikstas B., Earle E.D., Steponkus P.E. Cryopreservation of sweet potato shoot tips by vitrification. Cryobiology 1992, 29:738-739.
87. Shang Y.F., Yang C.L., Xin X.Q., Zhao J.H., Li C.S., Luo R.W. Techniques of sweetpotato virus-free by meristem tip culture. Plant Prot 1996, 22:14-16. (in Chinese).
88. Shimonishi K., Karube M., Ishikawa M. Cryopreservation of somatic embryos of sweet potato by slow prefreezing method. JIRCAS Int Agric Ser 2000, 8:368-370.
89. Sihachakr D., Harïcour R., Cavalcante Alves J.M., Umboh I., Nzoghe D., Servaes A., et al. Plant regeneration in sweetpotato (Ipomoea batatas L., Convolvulaceae). Euphytica 1997, 96:143-152.
90. Steponkus P.L., Langis R., Fujikawa S. Cryopreservation of plant tissues by vitrification. Advances in low temperature biology 1992, 1:1-61. JAI Press Ltd, London. P.L. Steponkus (Ed.).
91. Tairo F., Mukasa S.B., Jones R.A., Kullaya A., Rubaihayo P.R., Valkonen J.P.T. Unravelling the genetic diversity of the three main viruses invloved in sweetpotato virus disease (SPVD), and its practical implications. Mol Plant Pathol 2005, 6:199-211.
92. Tannoury M., Ralambosoa J., Kaminski M., Dereuddre J. Cryopreservation by vitrification of coated shoot tips of carnation (Diathus caryophyllus L.) cultured in vitro. C R Acad Sci Paris Ser III 1991, 313:633-638.
93. Torres A.C., Ze N.M., Cantliffe D.J. Abscisic acid and osmotic induction of synchronous somatic embryo development of sweetpotato. In Vitro Cell Dev Biol Plant 2001, 37:262-267.
94. Towill L.E., Bajaj Y.P.S. Cryopreservation of plant germplasm II. Biotechnology in agriculture and forestry 2002, 50. Springer-Verlag, Berlin. L.E. Towill, Y.P.S. Bajaj (Eds.).
95. Towill L.E., Jarret R.L. Cryopreservation of sweet potato (Ipomoea batatas (L.) Lam.) shoot tips by vitrification. Plant Cell Rep 1992, 11:175-178.
96. Tsay H.-S., Tseng M.-T. Embryoid formation and plantlet regeneration from anther callus of sweet potato. Bot Bull Acad Sin 1979, 20:117-122.
97. Wang Q.C., Perl A. Cryopreservation in floricultural crops. Floricultural, ornamental and plant biotechnology: advances and topics 2006, 523-539. Global Science Books, London. J.T. Silva (Ed.).
98. Wang Q.C., Valkonen J.P.T. Elimination of viruses and phytoplasma by cryotherapy of in vitro-grown shoot tips: analysis of all cases. Adv Hortic Sci 2007, 21:265-269.
99. Wang Q.C., Valkonen J.P.T. Efficient elimination of sweetpotato little leaf phytoplasma from sweetpotato by cryotherapy of shoot tips. Plant Pathol 2008, 57:338-347.
100. Wang Q.C., Valkonen J.P.T. Elimination of two viruses which interact synergistically from sweetpotato by shoot tip culture and cryotherapy. J Virol Meth 2008, 154:135-145.
101. Wang Q.C., Valkonen J.P.T. Cryotherapy of shoot tips: novel pathogen eradication. Trends Plant Sci 2009, 14:119-122.
102. Wang G.L., Fang H.J., Li H.Y. Somatic embryogenesis of sweetpotato and its applications to production of virus-free plants. Acta Agron Sin 2003, 29:345-348. (in Chinese, English abstract).
103. Wang Q.C., Mawassi M., Li P., Gafny R., Sela I., Tanne E. Elimination of grapevine virus A (GVA) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L. Plant Sci 2003, 165:321-327.
104. Wang Q.C., Laamanen J., Uosukainen M., Valkonen J.P.T. Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation-vitrification and encapsulation-dehydration. Plant Cell Rep 2005, 24:280-288.
105. Wang Q.C., Liu Y., Xie Y., You M. Cryotherapy of potato shoot tips for efficient elimination of potato leaf roll virus (PLRV) and potato virus Y (PVY). Potato Res 2006, 49:119-129.
106. Wang Q.C., Cuellar W.J., Rajamki M.L., Hiraka Y., Valkonen J.P.T. Combined thermotherapy and cryotherapy for virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips to efficient production of virus-free plants. Mol Plant Pathol 2008, 9:237-250.
107. Wang Q.C., Panis B., Engelmann F., Lambardi M., Valkonen J.P.T. Cryotherapy of shoot tips: a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation. Ann Appl Biol 2009, 154:351-363.
108. Wang Q.M., Zhang L.M., Wang B., Yin Z.F., Feng C.H., Wang Q.C. Sweetpotato viruses in China. Crop Prot 2010, 29:110-114.
109. Wilkinson T., Wetten A., Prychid C., Fay M. Suitability of cryopreservation for the long-term storage of rare and endangered plant species: a case history for Cosmos atrosanguineus. Ann Bot 2003, 91:65-74.
110. Yi G., Shin Y.-M., Choe G., Shin B., Soon Y.S., Kim Y.-M. Production of herbicide-resistant sweet potato plants transformed with the bar gene. Biotechnol Lett 2007, 29:669-675.
111. Yin ZF, Feng CH, Wang B, Engelmann F, Lambardi M, Panis B, et al. Cryotherapy of shoot tips: a newly emerging technique for efficient elimination of plant pathogens. Acta Hort in press.
112. Yoshinaga M., Yamakawa O. Cryopreservation of sweetpotato somatic embryos involving a desiccation step. Jpn J Breed 1994, 44(Supplement 4):290. (in Japanese).
113. Yu B., Zhai H., Wang Y.P., Zang N., He S.Z., Liu Q.C. Efficient Agrobacterium tumefaciens-mediated transformation using embryogenic suspension cultures in sweetpotato, Ipomoea batatas (L.) Lam. Plant Cell Tissue Organ Cult 2007, 90:265-273.
114. Zhang L.M., Wang Q.M., Liu Q.C., Wang Q.C. Sweetpotato in China. Biology and biotechnology of sweetpotato 2009, 325-358. Springer. G. Loebenstein, G. Thottappilly (Eds.).
115. Zheng Q., Dessai A.P., Prakash C.S. Rapid and repetitive plant regeneration in sweetpotato via somatic embryogenesis. Plant Cell Rep 1996, 15:381-385.