Vitrification-cryopreservation, an efficient method for eliminating Candidatus Liberobacter asiaticus, the citrus Huanglongbing pathogen, from in vitro adult shoot tips

Plant Cell Reports

Volumn 27, Issue 2, 2008, Pages 241-250

  Ding, Fang ^a,b   Jin, Shuangxia ^a   Hong, Ni ^a   Zhong, Yun ^b   Cao, Qing ^b,c   Yi, Ganjun ^b   Wang, Guoping ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF ANIMAL SCIENCE   (China)

^c NANCHANG INSTITUTE OF TECHNOLOGY   (China)

Author keywords

Citrus; Cryopreservation; Electron and light microscopy; HLB bacterium; Meristem culture; Vitrification

Indexed keywords

ARTICLE; CITRUS FRUIT; CRYOPRESERVATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METHODOLOGY; MICROBIOLOGY; MICROSCOPY; PLANT; POLYMERASE CHAIN REACTION; RHIZOBIACEAE; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

CITRUS; CRYOPRESERVATION; MICROSCOPY; MICROSCOPY, ELECTRON, TRANSMISSION; PLANT SHOOTS; POLYMERASE CHAIN REACTION; RHIZOBIACEAE;

BACTERIA (MICROORGANISMS); CANDIDATUS LIBERIBACTER ASIATICUS; CITRUS;

EID: 38349112610     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-007-0467-8     Document Type: Article

References (24)

1. Bové JM (2006) Huanglongbing: a destructive newly-emerging century-old disease of citrus. J Plant Pathol 88:7-37
2. Brison M, Boucaud MT, Pierronnet A, Dosba F (1997) Effect of cryopreservation on the sanitary state of a cv. Prunus rootstock experimentally contaminated with Plum Pox Potyvirus, Plant Sci 123:189-196
3. Ding F, Yi GJ, Wang GP (2004) Research on the PCR and nested-PCR detection of Citrus Huanglongbing Pathogen. Acta Horticult Sinica 31(6):803-806
4. Ding F, Wang GP, Yi GJ (2005) Infection of wampee and lemon by the citrus Huanglongbing pathogen (Candidatus liberibacter asiaticus) in China. J Plant Pathol 87(3):207-212
5. Engelmann F (1997) In vitro conservation methods. In: Callow JA, Ford-Lloyd BV, Newbury HJ (eds) Biotechnology and plant genetic resources. CAB International, Oxford, pp. 119-161
6. Faccioli G, Marani F (1998) Virus elimination by meristem tip culture and tip micrografting. In: Hadid A, Khetarpal ARK, Koganezawa H (eds) Plant virus diseases control. American Phytopathological Society, St. Paul, pp. 346-380
7. Garnier M, Danel N, Bovè JM (1984) Aetiology of citrus greening disease. Ann Microbiol 135A:169-179
8. Goheen AC (1988) Diseases caused by viruses and virus-like agents. In: Pearson RC, Goheen AC (eds) Compendium of grape diseases, American Phytopathological Society, St. Paul, pp. 47-48
9. Haskins RH, Kartha KK (1980) Freeze preservation of pea meristems: cell survival. Can J Bot 58:833-840
10. Helliot B (1998) Croissance et Stabilite genetique des Vitroplants de Prunier Ferlenain Plumina (R) apres cryoconservation des meristemes. PhD thesis, Universite de Bordeaux 1, France
11. Helliot B, Panis B, Poumay Y, Swenen R., Lepoivre P, Frison E (2002) Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.). Plant Cell Rep 20:1117-1122
12. Helliot B, Swennen R, Poumay Y, Frison E, Lepoivre P, Panis B (2003) Ultrastructural changes associated with cryopreservation of banana (Musa spp.) highly proliferating meristems. Plant Cell Rep 21:690-698
13. Jagoueix S, Bovè JM, Garnier M (1994) The phloem-limited bacterium of greening disease is a member of the a-subdivision of the Proteobacteria. Int J syst Bacteriol 44:379-386
14. Jagoueix S, Bovè JM, Garnier M (1997) Comparison of the 16S/23S ribosomal intergenic region of 'Candidatus liberobacter asiaticum' and 'Candidatus liberobacter africanum', the two species associated with citrus Huanglongbing (greening) disease. Int J Syst Bacteriol 47:224-227
15. Leonhardt W, Wawrosch C.H, Auer A, Kopp B (1998) Monitoring of virus diseases in Austrian grapevine varieties and virus elimination using in vitro thermotherapy, Plant Cell Tissue Organ Cult 52:71-74
16. Luo ZD (1991) Preliminary report on the efficacy of heated tetracycline hydrochloride solution in treating citrus yellow shoot disease. J South China Agric Univ 12(2):38-42
17. Murashge T, Tucker DPH (1969) Growth factor requirements of citrus tissue culture. In: Chapman HD (eds) Proceedings on 1st international citrus symposium, vol 3. International society citriculture (ISC), River-side, pp 1155-1161
18. Reynolds ES (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208-214
19. Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Obs. var. Brasiliensis tanaka) by vitrification. Plant Cell Rep 9:30-33
20. Song RL, Wu RJ, Ke C (1999) Elimination of main citrus virus and virus like disease by shoot tip grafting. Acta phytopathol sinica 29:275-279
21. Tannoury M, Vintejoux C (1997) Etudes cytologiques de bourgeonsd'oeillet (Dianthus caryophyllus L.) apres cryoconservation. Acta Bot Gall 144:107-118
22. Wang QC, Mawassi M, Li P, Gafny R, Sela I, Tanne E (2003) Elimination of grapevine virus A (HLB) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L. Plant Sci 165:321-327
23. Zhao XY, Qiu ZS, Su WF, Jiang YH (1981) Response of citrus huanglongbing to penicillin and tetracycline. South China Fruits (4):17
24. Zhao XY, Jiang YH, Li SL, Chen JZ, Ouyang L (1986) Indexing and exclusion of exocortis in Chinese citrus cultivars. Acta Horticult Sinica 13(2):91-94