Fluorescent detection of (-)-epicatechin in microsamples from cacao seeds and cocoa products: Comparison with Folin-Ciocalteu method

Journal of Food Composition and Analysis

Volumn 23, Issue 8, 2010, Pages 790-793

  Ramirez Sanchez, Israel ^a,b   Maya, Lisandro ^a   Ceballos, Guillermo ^a,b   Villarreal, Francisco ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^b INSTITUTO POLITÉCNICO NACIONAL   (Mexico)

Author keywords

( ) Epicatechin; Cacao seed; Catechin; Cocoa products; Flavanols; Flavonoids; Fluorescence; Folin Ciocalteu; Food analysis; Food composition; Phenolics; Rapid method of analysis; Theobroma cacao

Indexed keywords

THEOBROMA CACAO;

EID: 78649803672     PISSN: 08891575     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jfca.2010.03.014     Document Type: Article

References (13)

1. Ainsworth E.A., Gillespie K.M. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nature Protocols 2007, 2(4):875-877.
2. Budini R., Tonelli D., Girotti S. Analysis of total phenols using the Prussian Blue method. Journal of Agricultural and Food Chemistry 1980, 28:1236-1238.
3. Cho D., Mattice W., Porter L., Hemingway R. Use of the fluorescence quantum yield for the determination of the number-average molecular weight of polymers of (-)-epicatechin with 4β-8 interflavan. Polymer 1989, 30:1955-1958.
4. Cicco N., Lanorte M., Paraggio M., Viggiano M., Lattanzio V. A reproducible, rapid and inexpensive Folin-Ciocalteu micro-method in determining phenolics of plant methanol extracts. Microchemical Journal 2009, 91(1):107-110.
5. Cienfuegos-Jovellanos E., Quinones Mdel M., Muguerza B., Moulay L., Miguel M., Aleixandre A. Antihypertensive effect of a polyphenol-rich cocoa powder industrially processed to preserve the original flavonoids of the cocoa beans. Journal of Agricultural and Food Chemistry 2009, 57:6156-6162.
6. Corti R., Flammer A.J., Hollenberg N.K., Luscher T.F. Cocoa and cardiovascular health. Circulation 2009, 119:1433-1441.
7. Saint Joseph LECO Corporation Rapid Screening of Flavonoids in Cocoa using LC-TOFMS 2008.
8. Natsume M., Osakabe N., Yamagishi M., Takizawa T., Nakamura T., Miyatake H., Hatano T., Yoshida T. Analyses of polyphenols in cacao liquor, cocoa, and chocolate by normal-phase and reversed-phase HPLC. Bioscience Biotechnology and Biochemistry 2000, 64:2581-2587.
9. Roura E., Andres-Lacueva C., Estruch R., Lamuela-Raventos R.M. Total polyphenol intake estimated by a modified Folin-Ciocalteu assay of urine. Clinical Chemistry 2006, 52:749-752.
10. Sanbongi C., Osakabe N., Natsume M., Takizawa T., Gomi S., Osawa T. Antioxidative polyphenols isolated from Theobroma cacao. Journal of Agricultural and Food Chemistry 1998, 46:454-457.
11. Tomas-Barberan F.A., Cienfuegos-Jovellanos E., Marin A., Muguerza B., Gil-Izquierdo A., Cerda B., Zafrilla P., Morillas J., Mulero J., Ibarra A., Pasamar M.A., Ramon D., Espin J.C. A new process to develop a cocoa powder with higher flavonoid monomer content and enhanced bioavailability in healthy humans. Journal of Agricultural and Food Chemistry 2007, 55:3926-3935.
12. Xu Y.C., Leung S.W., Yeung D.K., Hu L.H., Chen G.H., Che C.M., Man R.Y. Structure-activity relationships of flavonoids for vascular relaxation in porcine coronary artery. Phytochemistry 2007, 68:1179-1188.
13. Yamazaki K.G., Romero-Perez D., Barraza-Hidalgo M., Cruz M., Rivas M., Cortez-Gomez B., Ceballos G., Villarreal F. Short- and long-term effects of (-)-(-)-epicatechin on myocardial ischemia-reperfusion injury. American Journal of Physiology Heart Circulatory Physiology 2008, 295:H761-H767.