Homodimerization of the death-associated protein kinase catalytic domain: Development of a new small molecule fluorescent reporter

PLoS ONE

Volumn 5, Issue 11, 2010, Pages

  Zimmermann, Michael ^a,d   Atmanene, Cédric ^a   Xu, Qingyan ^a,b   Fouillen, Laetitia ^a   van Dorsselaer, Alain ^a   Bonnet, Dominique ^a   Marsol, Claire ^a   Hibert, Marcel ^a   Sanglier Cianferani, Sarah ^a   Pigault, Claire ^a   McNamara, Laurie K ^c   Martin Watterson, D ^c   Haiech, Jacques ^a   Kilhoffer, Marie Claude ^a  

^a UNIVERSITÉ DE STRASBOURG   (France)

^b XIAMEN UNIVERSITY   (China)

^c NORTHWESTERN UNIVERSITY   (United States)

^d ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; DEATH ASSOCIATED PROTEIN KINASE; HOMODIMER; PEPTIDE LIBRARY; APOPTOSIS REGULATORY PROTEIN; CALCIUM CALMODULIN DEPENDENT PROTEIN KINASE; DEATH-ASSOCIATED PROTEIN KINASE; FLUORESCENT DYE; PEPTIDE;

ALLOSTERISM; ANISOTROPY; ARTICLE; BINDING AFFINITY; BINDING SITE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DIMERIZATION; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVE SITE; FLUORESCENCE ANALYSIS; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; LIGHT SCATTERING; MOLECULAR LIBRARY; MOLECULAR PROBE; OLIGOMERIZATION; PROTEIN PROTEIN INTERACTION; STRUCTURE ANALYSIS; ULTRACENTRIFUGATION; ANIMAL; BINDING COMPETITION; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; FLUORESCENCE POLARIZATION; GENETICS; HUMAN; METABOLISM; MUTATION; PROTEIN BINDING; PROTEIN MULTIMERIZATION;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ANIMALS; APOPTOSIS REGULATORY PROTEINS; BINDING SITES; BINDING, COMPETITIVE; CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES; CATALYTIC DOMAIN; FLUORESCENCE POLARIZATION; FLUORESCENT DYES; HUMANS; MOLECULAR STRUCTURE; MUTATION; PEPTIDES; PROTEIN BINDING; PROTEIN MULTIMERIZATION; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SUBSTRATE SPECIFICITY;

EID: 78649798970     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0014120     Document Type: Article

References (19)

1. Deiss LP, Feinstein E, Berissi H, Cohen O, Kimchi A (1995) Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death. Genes Dev 9: 15-30.
2. Shohat G, Shani G, Eisenstein M, Kimchi A (2002) The DAP-kinase family of proteins: study of a novel group of calcium-regulated death-promoting kinases. Biochim Biophys Acta 1600: 45-50.
3. Velentza AV, Wainwright MS, Zasadzki M, Mirzoeva S, Schumacher AM, et al. (2003) An aminopyridazine-based inhibitor of a pro-apoptotic protein kinase attenuates hypoxia-ischemia induced acute brain injury. Bioorg Med Chem Lett 13: 3465-3470.
4. Tereshko V, Teplova M, Brunzelle J, Watterson DM, Egli M (2001) Crystal structures of the catalytic domain of human protein kinase associated with apoptosis and tumor suppression. Nat Struct Biol 8: 899-907.
5. McNamara LK, Watterson DM, Brunzelle JS (2009) Structural insight into nucleotide recognition by human death-associated protein kinase. Acta Crystallogr D Biol Crystallogr 65: 241-248.
6. de Diego I, Kuper J, Bakalova N, Kursula P, Wilmanns M (2010) Molecular basis of the death-associated protein kinase-calcium/calmodulin regulator complex. Sci Signal 3: ra6.
7. Velentza AV, Schumacher AM, Weiss C, Egli M, Watterson DM (2001) A protein kinase associated with apoptosis and tumor suppression: structure, activity, and discovery of peptide substrates. J Biol Chem 276: 38956-38965.
8. Schumacher AM, Schavocky JP, Velentza AV, Mirzoeva S, Watterson DM (2004) A calmodulin-regulated protein kinase linked to neuron survival is a substrate for the calmodulin-regulated death-associated protein kinase. Biochemistry 43: 8116-8124.
9. Shani G, Marash L, Gozuacik D, Bialik S, Teitelbaum L, et al. (2004) Deathassociated protein kinase phosphorylates ZIP kinase, forming a unique kinase hierarchy to activate its cell death functions. Mol Cell Biol 24: 8611-8626.
10. Kawai T, Matsumoto M, Takeda K, Sanjo H, Akira S (1998) ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol Cell Biol 18: 1642-1651.
11. Dagher R, Pigault C, Bonnet D, Boeglin D, Pourbaix C, et al. (2006) Use of a fluorescent polarization based high throughput assay to identify new calmodulin ligands. Biochim Biophys Acta 1763: 1250-1255.
12. Rink H (1987) Solid-phase synthesis of protected peptide fragments using a trialkoxy-diphenyl-methylester resin. Tetrahedron Letters 28: 3787-3790.
13. Chang CD, Meienhofer J (1978) Solid-phase peptide synthesis using mild base cleavage of N alpha-fluorenylmethyloxycarbonylamino acids, exemplified by a synthesis of dihydrosomatostatin. Int J Pept Protein Res 11: 246-249.
14. Hibert M, Franchet C, Galzi J-L, Pattus F, Guillier F (2006) Collection of traceable compounds and uses thereof. Patent;WO/2006/003330.
15. Laue TM, Shah BD, Ridgeway TM, Pelletier SL, eds (1992) Computer-aided interpretation of analytical sedimentation data for proteins. Cambridge: The Royal Society of Chemistry. pp 90-125.
16. Schuck P (2000) Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling. Biophys J 78: 1606-1619.
17. Garcia De La Torre J, Huertas ML, Carrasco B (2000) Calculation of hydrodynamic properties of globular proteins from their atomic-level structure. Biophys J 78: 719-730.
18. Kilhoffer MC, Kubina M, Travers F, Haiech J (1992) Use of engineered proteins with internal tryptophan reporter groups and pertubation techniques to probe the mechanism of ligand-protein interactions: investigation of the mechanism of calcium binding to calmodulin. Biochemistry 31: 8098-8106.
19. Roehrl MH, Wang JY, Wagner G (2004) A general framework for development and data analysis of competitive high-throughput screens for small-molecule inhibitors of protein-protein interactions by fluorescence polarization. Biochemistry 43: 16056-16066.