Genetic transformation and mutagenesis via single-stranded DNA in the unicellular, diazotrophic cyanobacteria of the genus cyanothece

Applied and Environmental Microbiology

Volumn 76, Issue 22, 2010, Pages 7641-7645

  Min, Hongtao ^a   Sherman, Louis A ^a  

^a PURDUE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE KNOCKOUT; GENETIC SYSTEMS; GENETIC TRANSFORMATION; SINGLE-STRANDED DNA;

NUCLEIC ACIDS;

HYDROGEN; SINGLE STRANDED DNA;

CYANOBACTERIUM; GENETIC ANALYSIS; HYDROGEN; MUTAGENICITY; NITROGEN;

ARTICLE; BACTERIAL GENE; CHEMISTRY; CULTURE MEDIUM; CYANOTHECE; GENE INACTIVATION; GENETIC TRANSFORMATION; GENETICS; METABOLISM; METHODOLOGY; MICROBIAL GENETICS; MUTAGENESIS;

CULTURE MEDIA; CYANOTHECE; DNA, SINGLE-STRANDED; GENE KNOCKOUT TECHNIQUES; GENES, BACTERIAL; GENETICS, MICROBIAL; HYDROGEN; MUTAGENESIS; TRANSFORMATION, GENETIC;

CYANOBACTERIA; CYANOTHECE;

EID: 78649711440     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01456-10     Document Type: Article

References (37)

1. 2 triple bond: Insights into the nitrogenase mechanism. Dalton Trans. 21:2277-2284.
2. 2. J. Am. Chem. Soc. 127:14960-14961.
3. Colón-López, M. S., D. M. Sherman, and L. A. Sherman. 1997. Transcriptional and translational regulation of nitrogenase in light-dark- and continuous- light-grown cultures of the unicellular cyanobacterium Cyanothece sp. strain ATCC 51142. J. Bacteriol. 179:4319-4327.
4. Colón-López, M. S., and L. A. Sherman. 1998. Transcriptional and translational regulation of photosystem I and II genes in light-dark- and continuous- light-grown cultures of the unicellular cyanobacterium Cyanothece sp. strain ATCC 51142. J. Bacteriol. 180:519-526.
5. Dos Santos, P. C., R. Y. Igarashi, H. I. Lee, B. M. Hoffman, L. C. Seefeldt, and D. R. Dean. 2005. Substrate interactions with the nitrogenase active site. Acc. Chem. Res. 38:208-214.
6. Elhai, J., T. Thiel, and H. B. Pakrasi. 1990. DNA transfer into cyanobacteria, p. 1-23. In S. B. Gelvin and R. A. Schilperoort (ed.), Plant molecular biology manual. Kluwer Academic Publishers, Dordrecht, Netherlands.
7. Elhai, J., A. Vepritskiy, A. M. Muro-Pastor, E. Flores, and C. P. Wolk. 1997. Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120. J. Bacteriol. 179:1998-2005.
8. Elhai, J., and C. P. Wolk. 1988. Conjugal transfer of DNA to cyanobacteria. Methods Enzymol. 167:747-754.
9. Elhai, J., and C. P. Wolk. 1988. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68:119-138.
10. Golden, S. S. 1988. Mutagenesis of cyanobacteria by classical and genetransfer- based methods. Methods Enzymol. 167:714-727.
11. Golden, S. S., and L. A. Sherman. 1984. Optimal conditions for genetic transformation of the cyanobacterium Anacystis nidulans R2. J. Bacteriol. 158:36-42.
12. Haselkorn, R. 1991. Genetic systems in cyanobacteria. Methods Enzymol. 204:418-430.
13. Haselkorn, R. 1995. Molecular genetics of nitrogen fixation in photosynthetic prokaryotes, p. 29-36. In I. A. Tikhonovich, N. A. Provorov, V. I. Romanov, and W. E. Newton (ed.), Nitrogen fixation: Fundamentals and applications. Kluwer Academic Publishers, Dordrecht, Netherlands.
14. Koksharova, O. A., and C. P. Wolk. 2002. Genetic tools for cyanobacteria. Appl. Microbiol. Biotechnol. 58:123-137.
15. 2 as substrates and inhibitors of nitrogenase from Azotobacter vinelandii. Biochemistry 27:6726-6732.
16. Mackey, S. R., and S. S. Golden. 2007. Winding up the cyanobacterial circadian clock. Trends Microbiol. 15:381-388.
17. Min, H., and L. A. Sherman. 2010. Hydrogen production by the unicellular, diazotrophic cyanobacterium Cyanothece sp. strain ATCC 51142 under conditions of continuous light. Appl. Environ. Microbiol. 76:4293-4301.
18. Parrish, J. R., T. Limjindaporn, J. A. Hines, J. Liu, G. Liu, and R. L. Finley, Jr. 2004. High-throughput cloning of Campylobacter jejuni ORFs by in vivo recombination in Escherichia coli. J. Proteome Res. 3:582-586.
19. Reddy, K. J., J. B. Haskell, D. M. Sherman, and L. A. Sherman. 1993. Unicellular, aerobic nitrogen-fixing cyanobacteria of the genus Cyanothece. J. Bacteriol. 175:1284-1292.
20. Schneegurt, M. A., D. M. Sherman, S. Nayar, and L. A. Sherman. 1994. Oscillating behavior of carbohydrate granule formation and dinitrogen fixation in the cyanobacterium Cyanothece sp. strain ATCC 51142. J. Bacteriol. 176:1586-1597.
21. Schneegurt, M. A., D. M. Sherman, and L. A. Sherman. 1997. Composition of the carbohydrate granules of the cyanobacterium, Cyanothece sp. strain ATCC 51142. Arch. Microbiol. 167:89-98.
22. Sherman, L. A., P. Meunier, and M. S. Colón-López. 1998. Diurnal rhythms in metabolism: A day in the life of a unicellular, diazotrophic cyanobacterium. Photosynth. Res. 58:25-42.
23. Simpson, F. B., and R. H. Burris. 1984. A nitrogen pressure of 50 atmospheres does not prevent evolution of hydrogen by nitrogenase. Science 224:1095-1097.
24. Stöckel, J., E. A. Welsh, M. Liberton, R. Kunnvakkam, R. Aurora, and H. B. Pakrasi. 2008. Global transcriptomic analysis of Cyanothece 51142 reveals robust diurnal oscillation of central metabolic processes. Proc. Natl. Acad. Sci. U. S. A. 105:6156-6161.
25. Thiel, T. 1994. Genetic analysis of cyanobacteria, p. 581-611. In D. A. Brant (ed.), The molecular biology of cyanobacteria. Kluwer Academic Publisher, Dordrecht, Netherlands.
26. Thiel, T., and H. Poo. 1989. Transformation of a filamentous cyanobacterium by electroporation. J. Bacteriol. 171:5743-5746.
27. Toepel, J., J. E. McDermott, T. C. Summerfield, and L. A. Sherman. 2009. Transcriptional analysis of the unicellular, diazotrophic cyanobacterium Cyanothece sp. ATCC 51142 grown under short day/night cycles. J. Phycol. 45:610-620.
28. Toepel, J., E. Welsh, T. C. Summerfield, H. B. Pakrasi, and L. A. Sherman. 2008. Differential transcriptional analysis of the cyanobacterium Cyanothece sp. strain ATCC 51142 during light-dark and continuous-light growth. J. Bacteriol. 190:3904-3913.
29. van der Oost, J., B. A. Bulthuis, S. Feitz, K. Krab, and R. Kraayenhof. 1989. Fermentation metabolism of the unicellular cyanobacterium Cyanothece PCC 7822. Arch. Microbiol. 152:415-419.
30. Vermaas, W. 1996. Molecular genetics of the cyanobacterium Synechocystis sp. PCC 6803: Principles and possible biotechnology applications. J. Appl. Phycol. 8:263-273.
31. Vermaas, W. F. 1998. Gene modifications and mutation mapping to study the function of photosystem II. Methods Enzymol. 297:293-310.
32. Welsh, E. A., M. Liberton, J. Stockel, T. Loh, T. Elvitigala, C. Wang, A. Wollam, R. S. Fulton, S. W. Clifton, J. M. Jacobs, R. Aurora, B. K. Ghosh, L. A. Sherman, R. D. Smith, R. K. Wilson, and H. B. Pakrasi. 2008. The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle. Proc. Natl. Acad. Sci. U. S. A. 105:15094-15099.
33. Williams, J. G., and A. A. Szalay. 1983. Stable integration of foreign DNA into the chromosome of the cyanobacterium Synechococcus R2. Gene 24:37-51.
34. Wolk, C. P. 1996. Heterocyst formation. Annu. Rev. Genet. 30:59-78.
35. Wolk, C. P., and J. Kraus. 1982. Two approaches to obtaining low, extracellular deoxyribonuclease activity in cultures of heterocyst-forming cyanobacteria. Arch. Microbiol. 131:302-307.
36. Wolk, C. P., A. Vonshak, P. Kehoe, and J. Elhai. 1984. Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen- fixing filamentous cyanobacteria. Proc. Natl. Acad. Sci. U. S. A. 81:1561-1565.
37. Zorin, B., P. Hegemann, and I. Sizova. 2005. Nuclear-gene targeting using single-stranded DNA avoids illegitimate DNA integration in Chlamydomonas reinhardtii. Eukaryot. Cell 4:1264-1272.