Incubated protein reduction and digestion on an electrowetting-on- dielectric digital microfluidic chip for MALDI-MS

Analytical Chemistry

Volumn 82, Issue 23, 2010, Pages 9932-9937

  Nelson, Wyatt C ^a   Peng, Ivory ^a   Lee, Geun An ^b   Loo, Joseph A ^a,c   Garrell, Robin L ^a   Kim, Chang Jin ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b Korea Institute of Industrial Technology   (South Korea)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHANGING TEMPERATURE; CHIP DESIGN; CLOSED-LOOP; CYTOCHROME C; DIGITAL MICROFLUIDICS; DISULFIDE REDUCTION; DITHIOTHREITOL; ELECTRO-WETTING ON DIELECTRICS; ELECTROWETTING-ON-DIELECTRIC CHIPS; HEATER TEMPERATURES; HIGH-BOILING POINT SOLVENTS; LIQUID DROPLETS; LOCALIZED HEATING; MALDI-MS; MATRIX; MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY; PEPTIDE SEQUENCES; SURFACE ELECTRODE; SYSTEM COMPLEXITY; TEMPERATURE SENSING; THERMAL CONTROL; TRYPSIN DIGESTION; TRYPTIC DIGESTION;

DESORPTION; DIELECTRIC DEVICES; DIMETHYL SULFOXIDE; DROP FORMATION; ELECTRODES; HEATING; INSULIN; MASS SPECTROMETRY; ORGANIC SOLVENTS; TEMPERATURE CONTROL; WETTING;

REACTION RATES;

CYTOCHROME C; DIMETHYL SULFOXIDE; INSULIN; TRYPSIN;

ARTICLE; CHEMISTRY; ELECTRODE; ELECTROWETTING; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MICROFLUIDIC ANALYSIS; OXIDATION REDUCTION REACTION; TEMPERATURE;

CYTOCHROMES C; DIMETHYL SULFOXIDE; ELECTRODES; ELECTROWETTING; INSULIN; MICROFLUIDIC ANALYTICAL TECHNIQUES; OXIDATION-REDUCTION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TEMPERATURE; TRYPSIN;

EID: 78649689794     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac101833b     Document Type: Article

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