Elevated linezolid resistance in clinical cfr-positive Staphylococcus aureus isolates is associated with co-occurring mutations in ribosomal protein L3

Antimicrobial Agents and Chemotherapy

Volumn 54, Issue 12, 2010, Pages 5352-5355

  Locke, Jeffrey B ^a   Morales, Gracia ^b   Hilgers, Mark ^a   Kedar, G C ^a   Rahawi, Shahad ^a   Picazo, Juan José ^b   Shaw, Karen Joy ^a   Stein, Jeffrey L ^a  

^a Trius Therapeutics Inc   (United States)

^b HOSPITAL CLÍNICO SAN CARLOS   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORAMPHENICOL; LINEZOLID; OXAZOLIDINONE DERIVATIVE; RADEZOLID; RIBOSOME PROTEIN; RIBOSOME PROTEIN L3; RX 1741; TIAMULIN; TOREZOLID; TR 700; UNCLASSIFIED DRUG; VANCOMYCIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM ISOLATE; CHEMICAL STRUCTURE; EPIDEMIC; GENE MUTATION; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; SPAIN; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS INFECTION;

ACETAMIDES; ANTI-INFECTIVE AGENTS; BACTERIAL PROTEINS; DRUG RESISTANCE, BACTERIAL; METHYLTRANSFERASES; MICROBIAL SENSITIVITY TESTS; MUTATION; OXAZOLIDINONES; RIBOSOMAL PROTEINS; STAPHYLOCOCCUS AUREUS;

EID: 78649660049     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.00714-10     Document Type: Article

References (32)

1. Bonilla, H., J. P. Quinn, J. Siedel, H. Schmidt, M. Lescoe, S. P. McCurdy, M. M. Lemmon, M. D. Huband, L. Puzniak, T. Hart, K. Jafri, L. A. Brennan, and A. Tait-Kamradt. 2009. Abstr. 49th Intersci. Conf. Antimicrob. Agents Chemother., abstr. C2-131. American Society for Microbiology, Washington, DC.
2. Cercenado, E., M. Marin, R. Insa, and E. Bouza. 2010. Abstr. 20th European Congress of Clinical Microbiology and Infectious Diseases, abstr. P979. European Society of Clinical Microbiology and Infectious Diseases, Munich, Germany.
3. CLSI. 2006. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard, CLSI Document M7-A7, 7th ed., vol. 26, no. 2. CLSI, Wayne, PA.
4. DeLano, W. L. 2002. The PyMOL molecular graphics system. DeLano Scientific, Palo Alto, CA.
5. Farrell, D. J., R. E. Mendes, J. E. Ross, and R. N. Jones. 2009. Linezolid surveillance program results for 2008 (LEADER Program for 2008). Diagn. Microbiol. Infect. Dis. 65:392-403.
6. Giessing, A. M., S. S. Jensen, A. Rasmussen, L. H. Hansen, A. Gondela, K. Long, B. Vester, and F. Kirpekar. 2009. Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria. RNA 15:327-336.
7. Gonzales, R. D., P. C. Schreckenberger, M. B. Graham, S. Kelkar, K. DenBesten, and J. P. Quinn. 2001. Infections due to vancomycin-resistant Enterococcus faecium resistant to linezolid. Lancet 357:1179.
8. Holzel, C. S., K. S. Harms, K. Schwaiger, and J. Bauer. 2010. Resistance to linezolid in a porcine Clostridium perfringens strain carrying a mutation in the rplD gene encoding the ribosomal protein L4. Antimicrob. Agents Chemother. 54:1351-1353.
9. Jones, R. N., S. Kohno, Y. Ono, J. E. Ross, and K. Yanagihara. 2009. ZAAPS International Surveillance Program (2007) for linezolid resistance: results from 5591 Gram-positive clinical isolates in 23 countries. Diagn. Microbiol. Infect. Dis. 64:191-201.
10. Jones, R. N., J. E. Ross, J. M. Bell, U. Utsuki, I. Fumiaki, I. Kobayashi, and J. D. Turnidge. 2009. Zyvox annual appraisal of potency and spectrum program: linezolid surveillance program results for 2008. Diagn. Microbiol. Infect. Dis. 65:404-413.
11. Kehrenberg, C., F. M. Aarestrup, and S. Schwarz. 2007. IS21-558 insertion sequences are involved in the mobility of the multiresistance gene cfr. Antimicrob. Agents Chemother. 51:483-487. (Pubitemid 46185264)
12. LaMarre, J. M., T. Zhu, J. B. Locke, K. J. Shaw, and A. S. Mankin. 2009. Abstr. 49th Intersci. Conf. Antimicrob. Agents Chemother., abstr. C1-1346. American Society for Micriobiology, Washington, DC.
13. Lawrence, L., P. Danese, J. DeVito, F. Franceschi, and J. Sutcliffe. 2008. In vitro activities of the Rx-01 oxazolidinones against hospital and community pathogens. Antimicrob. Agents Chemother. 52:1653-1662.
14. Locke, J. B., J. Finn, M. Hilgers, G. Morales, S. Rahawi, K. G. C., J. J. Picazo, W. Im, K. J. Shaw, and J. L. Stein. 2010. Structure-activity relationships of diverse oxazolidinones for linezolid-resistant Staphylococcus aureus strains possessing the cfr methyltransferase gene or ribosomal mutations. Antimicrob. Agents Chemother. 54:5337-5343.
15. Locke, J. B., M. Hilgers, and K. J. Shaw. 2009. Mutations in ribosomal protein L3 are associated with oxazolidinone resistance in staphylococci of clinical origin. Antimicrob. Agents Chemother. 53:5275-5278.
16. Locke, J. B., M. Hilgers, and K. J. Shaw. 2009. Abstr. 49th Intersci. Conf. Antimicrob. Agents Chemother., abstr. C1-1349. American Society for Microbiology, Washington, DC.
17. Locke, J. B., M. Hilgers, and K. J. Shaw. 2009. Novel ribosomal mutations in Staphylococcus aureus strains identified through selection with the oxazolidinones linezolid and torezolid (TR-700). Antimicrob. Agents Chemother. 53:5265-5274.
18. Locke, J. B., A. S. Mankin, J. LaMarre, V. Brown-Driver, and K. J. Shaw. 2009. Abstr. 49th Intersci. Conf. Antimicrob. Agents Chemother., abstr. C1-1364b. American Society for Microbiology, Washington, DC.
19. Long, K. S., J. Poehlsgaard, C. Kehrenberg, S. Schwarz, and B. Vester. 2006. The cfr rRNA methyltransferase confers resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. Antimicrob. Agents Chemother. 50:2500-2505.
20. Meka, V. G., S. K. Pillai, G. Sakoulas, C. Wennersten, L. Venkataraman, P. C. DeGirolami, G. M. Eliopoulos, R. C. Moellering, and H. S. Gold. 2004. Linezolid resistance in sequential Staphylococcus aureus isolates associated with a T2500A mutation in the 23S rRNA gene and loss of a single copy of rRNA. J. Infect. Dis. 190:311-317.
21. Mendes, R. E., L. M. Deshpande, M. Castanheira, J. Dipersio, M. Saubolle, and R. N. Jones. 2008. First report of cfr-mediated resistance to linezolid in human staphylococcal clinical isolates recovered in the United States. Antimicrob. Agents Chemother. 52:2244-2246.
22. Morales, G., and J. J. Picazo. 2009. Abstr. 49th Intersci. Conf. Antimicrob. Agents Chemother., abstr. E-808. American Society for Microbiology, Washington, DC.
23. Morales, G., J. J. Picazo, E. Baos, F. J. Candel, A. Arribi, B. Pelaez, R. Andrade, M. A. de la Torre, J. Fereres, and M. Sanchez-Garcia. 2010. Resistance to linezolid is mediated by the cfr gene in the first report of an outbreak of linezolid-resistant Staphylococcus aureus. Clin. Infect. Dis. 50:821-825.
24. Schenk, S., and R. A. Laddaga. 1992. Improved method for electroporation of Staphylococcus aureus. FEMS Microbiol. Lett. 73:133-138.
25. Schwarz, S., C. Werckenthin, and C. Kehrenberg. 2000. Identification of a plasmid-borne chloramphenicol-florfenicol resistance gene in Staphylococcus sciuri. Antimicrob. Agents Chemother. 44:2530-2533.
26. Shaw, K. J., S. Poppe, R. Schaadt, V. Brown-Driver, J. Finn, C. M. Pillar, D. Shinabarger, and G. Zurenko. 2008. In vitro activity of TR-700, the antibacterial moiety of the prodrug TR-701, against linezolid-resistant strains. Antimicrob. Agents Chemother. 52:4442-4447.
27. Skripkin, E., T. S. McConnell, J. Devito, L. Lawrence, J. A. Ippolito, E. M. Duffy, J. Sutcliffe, and F. Franceschi. 2008. Rx-01: a new family of oxazolidinones that overcomes ribosomal-based linezolid resistance. Antimicrob. Agents Chemother. 52:3550-3557.
28. Smith, L. K., and A. S. Mankin. 2008. Transcriptional and translational control of the mlr operon, which confers resistance to seven classes of protein synthesis inhibitors. Antimicrob. Agents Chemother. 52:1703-1712.
29. Toh, S. M., L. Xiong, C. A. Arias, M. V. Villegas, K. Lolans, J. Quinn, and A. S. Mankin. 2007. Acquisition of a natural resistance gene renders a clinical strain of methicillin-resistant Staphylococcus aureus resistant to the synthetic antibiotic linezolid. Mol. Microbiol. 64:1506-1514.
30. Tsiodras, S., H. S. Gold, G. Sakoulas, G. M. Eliopoulos, C. Wennersten, L. Venkataraman, R. C. Moellering, and M. J. Ferraro. 2001. Linezolid resistance in a clinical isolate of Staphylococcus aureus. Lancet 38:207-208.
31. Wilson, D. N., F. Schluenzen, J. M. Harms, A. L. Starosta, S. R. Connell, and P. Fucini. 2008. The oxazolidinone antibiotics perturb the ribosomal peptidyl-transferase center and effect tRNA positioning. Proc. Natl. Acad. Sci. U. S. A. 105:13339-13344.
32. Wolter, N., A. M. Smith, D. J. Farrell, W. Schaffner, M. Moore, C. G. Whitney, J. H. Jorgensen, and K. P. Klugman. 2005. Novel mechanism of resistance to oxazolidinones, macrolides, and chloramphenicol in ribosomal protein L4 of the pneumococcus. Antimicrob. Agents Chemother. 49:3554-3557.