Different strategies for preparation of non-tagged rV270 protein and its efficacy against Yersinia pestis challenge

Biomedical and Environmental Sciences

Volumn 23, Issue 5, 2010, Pages 333-340

  Wang, Wang ^a   Qi, Zhi Zhen ^b   Zhang, Qing Wen ^b   Wu, Ben Chuan ^c   Zhu, Zi Wen ^a   Yang, Yong Hai ^b   Cui, Bai Zhong ^b   Dai, Rui Xia ^b   Qiu, Ye Feng ^a   Wang, Zu Yun ^b   Guo, Zhao Biao ^a   Shi, Tao Xing ^a   Wang, Hu ^b   Yang, Rui Fu ^a   Wang, Xiao Yi ^a  

^a BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

^b Qinghai Institute for Endemic Disease Prevention and Control   (China)

^c Beijing Institute of Bioengneering   (China)

Author keywords

Plague; Protection; Purification; rV270 antigen; Yersinia pestis

Indexed keywords

BACTERIAL ANTIGEN; BACTERIUM ANTIBODY; HYBRID PROTEIN; LCRV PROTEIN, YERSINIA; PLAGUE VACCINE; PORE FORMING CYTOTOXIC PROTEIN; SUBUNIT VACCINE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BAGG ALBINO MOUSE; BLOOD; ESCHERICHIA COLI; FEMALE; GENE VECTOR; GENETICS; GROWTH, DEVELOPMENT AND AGING; IMMUNOLOGY; MASS SPECTROMETRY; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; MOUSE; PLAGUE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ENGINEERING; SURVIVAL; WESTERN BLOTTING; YERSINIA PESTIS;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; BLOTTING, WESTERN; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FEMALE; GENETIC VECTORS; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; PLAGUE; PLAGUE VACCINE; PLASMIDS; PORE FORMING CYTOTOXIC PROTEINS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SURVIVAL ANALYSIS; VACCINES, SUBUNIT; YERSINIA PESTIS;

MUS; YERSINIA PESTIS;

EID: 78649626106     PISSN: 08953988     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0895-3988(10)60073-6     Document Type: Article

References (19)

1. RD Perry, JD Fetherston Yersinia pestis-etiologic agent of plague Clin Microbiol Rev 10 1 1997 35 66
2. S Riedel Plague: from natural disease to bioterrorism Proc (Bayl Univ Med Cent) 18 2 2005 116 124
3. RW Titball, ED Williamson Yersinia pestis (plague) vaccines Expert Opin Biol Ther 4 6 2004 965 973
4. KA Overheim, RW Depaolo, KL Debord LcrV plague vaccine with altered immunomodulatory properties Infect Immun 73 8 2005 5152 5159
5. KL DeBord, DM Anderson, MM Marketon Immunogenicity and protective immunity against bubonic plague and pneumonic plague by immunization of mice with the recombinant V10 antigen, a variant of LcrV Infect Immun 74 8 2006 4910 4914
6. K Terpe Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems Appl Microbiol Biotechnol 60 2003 523 533
7. B Rasoamanana, F Leroy, P Boisier Field evaluation of an immunoglobulin G anti-F1 enzyme-linked immunosorbent assay for serodiagnosis of human plague in Madagascar Clin Diagn Lab Immunol 4 5 1997 587 591
8. ED Williamson, AJ Stagg, SM Eley Kinetics of the immune response to the (F1+V) vaccine in models of bubonic and pneumonic plague Vaccine 25 6 2007 1142 1148
9. R Dakshinamurthy, A Koteshwara, D Paul A method for the prevention of thrombin-induced degradation of recombinant proteins Aanl Biochem 375 2008 361 363
10. K Nagai, HC Thogersen Generation of beta-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli Nature 309 5971 1984 810 812
11. TJ Kim, S Chauhan, VL Motin Direct transcriptional control of the plasminogen activator gene of Yersinia pestis by the cyclic AMP receptor protein J Bacteriol 189 24 2007 8890 8900
12. M Kenig, S Peternel, V Gaberc-Porekar Influence of the protein oligomericity on final yield after affinity tag removal in purification of recombinant proteins J Chromatogr A 1101 1-2 2006 293 306
13. J Hill, SE Leary, KF Griffin Regions of Yersinia pestis V antigen that contribute to protection against plague identified by passive and active immunization Infect Immun 65 11 1997 4476 4482
14. A Sodhi, RK Sharma, HV Batra Mechanism of rLcrV and rYopB mediated Immunosuppression in murine peritoneal macrophages Mol Immunol 41 8 2004 767 774 (Pubitemid 38953634)
15. Y Du, R Rosqvist, A Forsberg Role of fraction 1 antigen of Yersinia pestis in inhibition of phagocytosis Infect Immun 70 3 2002 1453 1460
16. C Cowan, AV Philipovskiy, CR Wulff-Strobel Anti-LcrV antibody inhibits delivery of Yops by Yersinia pestis KIM5 by directly promoting phagocytosis Infect Immun 73 9 2005 6127 6137
17. C Pujol, JB Bliska Turning Yersinia pathogenesis outside in: subversion of macrophage function by intracellular yersiniae Clin Immunol 114 3 2005 216 226
18. AV Philipovskiy, ST Smiley Vaccination with Live Yersinia pestis Primes CD4 and CD8 T Cells That Synergistically Protect against Lethal Pulmonary Y. pestis Infection Infect Immun 75 2 2007 878 885
19. ST Smiley Immune defense against pneumonic plague Immunological Reviews 225 2008 256 271